Custom synthesis of pDNA involves chemically synthesizing target DNA sequences into synthetic DNA molecules according to specific design requirements. BOC Sciences offers a variety of production tools to facilitate efficient pDNA production. Explore the process of plasmid DNA production from bacteria to final filtration and discover solutions to assist you at every step of the process.
Plasmid DNA (pDNA) is an important starting point for many genetic engineering studies, including the development of recombinant proteins, viral vectors and advanced biotherapeutics. pDNA can be replicated independently (free-form) from bacterial genomic DNA, a property that makes them ideally suited as vectors for genetic engineering.
Fig 1. Schematic of pDNA vectors and DNA minicircles. (H Sum et al., 2014)
The target gene is inserted into the pDNA and then introduced into the host bacteria by electroporation. The host cell replicates during fermentation, replicating the plasmid and obtaining the desired sequence in high yield. Once the cells reach the desired density, the cells are harvested and the pDNA is extracted. The lysate is then clarified and the pDNA is purified using chromatography.
The types of pDNA production we can currently offer include the following.
| Research grade pDNA | Transfection grade pDNA | Quasi-medical pDNA |
| Research grade pDNA can be used for molecular cloning, targeted mutagenesis, bacterial transformation and other basic molecular biology experiments. | Transfection grade pDNA can be used for cell transfection, gene vaccine, gene therapy and other high-demand experiments. | For preclinical use in cell therapy, gene therapy, etc. |
BOC Sciences' biotechnology platform is able to optimize the sequences you need, according to your requirements, for any optimization host of your choice.
In pDNA manufacturing production, the bacterial fluids are collected first, followed by alkaline lysis of the phage and finally removal of impurities, taking into account efficiency and amplification. pDNA manufacturing proceeds as follows.
Vector construction - Seed bank establishment - Fermentation - Phage collection - Solid-liquid separation of lysed cells - Clarification and concentration - Isolation and purification - Product quality control - Dispensing
High-quality plasmid DNA is a key component of gene therapy production and is in high demand, requiring optimization of the production purification process to meet the quality requirements needed for therapeutic drug production. Quality control of plasmid DNA includes the following.
BOC Sciences provides professional pDNA preparation and scale-up production services to help you complete your pDNA synthesis project efficiently. If you are interested in our services, please feel free to contact us.
It is the process of chemically constructing and cloning specific DNA sequences into plasmid vectors. This provides a tailored genetic template for your research or development projects.
We provide research grade for molecular cloning, transfection grade for cell-based assays, and quasi-medical grade for preclinical studies. Each grade is produced with specific purity and endotoxin controls.
We employ multiple chromatography steps and stringent quality control, including sequencing and structural analysis. This ensures high purity with ultra-low endotoxin levels for demanding applications.
It begins with vector construction and progresses through fermentation, harvesting, and advanced purification. Our scalable process supports projects from milligram to gram quantities.
Our pDNA is used in genetic engineering, recombinant protein production, and as starting material for viral vector development. It serves fundamental roles in biotechnology research.
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