The DNA-Directed RNA Interference (ddRNAi) service provided by BOC Sciences is an improved service based on RNAi technology. ddRNAi service includes gene silencing, gene expression regulation, etc., which can be widely used in disease modeling and drug discovery.
DNA-Directed RNA Interference (ddRNAi) is a gene silencing technique used to inhibit the expression of specific genes. ddRNAi methodology relies on the use of an introduced DNA template to utilize the cell's endogenous transcriptional machinery to produce a short hairpin RNA (shRNA), which is then processed by the endogenous RNAi machinery into a siRNA. Once the siRNA is loaded into the RNA-induced silencing complex (RISC), the corresponding antisense strand can bind to the target RNA, ultimately leading to the loss of the messenger RNA. Since no mRNA species can be translated into protein, the net effect of RNAi is to induce a loss of gene expression of the target gene.
ddRNAi works by introducing DNA sequences of specific genes into cells and transcribing them into RNA molecules. The antisense sequences in these RNA molecules can be complementarily paired with the mRNA sequences of the target gene and form an RNA double-stranded structure. Then, these small molecules can further form RISC and recognize and target the corresponding mRNAs in the cell through the guidance of RISC, leading to the degradation or inhibition of the target gene. As a result, the expression level of the target gene will decrease, thus realizing the effect of gene silencing.
BOC Sciences' ddRNAi service provides a precise, long-lasting, and efficient approach to gene silencing and expression regulation. The gene silencing service can be realized under the mediation of siRNA, and then, by adjusting the design of ddRNAi constructs, gene expression can be enhanced or suppressed. Gene expression can be enhanced or suppressed by adjusting the design of ddRNAi constructs.
|The promoter is the region used to drive the expression of the ddRNAi construct, and a strong promoter can produce higher levels of ddRNAi.
|Formation of short circular RNA structures
|Sequences in ddRNAi constructs should form a short circular RNA structure (consisting of an antisense strand and a linker strand).
|Length/sequence of linker and antisense strand
|The length of the linker and antisense strand should be between 15-30 base pairs to ensure that it is long enough to form a stable loop structure.
|ddRNAi constructs often need to be cloned into appropriate background vectors to facilitate expression and delivery in target cells.