BOC RNA provides long oligonucleotide synthesis services. Based on our oligonucleotide synthesis platform, we provide our customers with efficient long oligonucleotides for cloning, mutagenesis, gene expression and other applications.
The emergence of gene synthesis and targeted mutagenesis has driven the need to synthesize long oligonucleotides (>75 nucleotides). While there is a high demand for long oligonucleotides (150 mer and more), the yields and associated side reactions of conventional DNA synthesis previously limited the availability of oligonucleotides to<150 nucleotides. The longer the oligonucleotide, the more difficult it is to isolate the pure bioactive product. No chemical reaction can be completed with 100% efficiency. In oligonucleotide synthesis reactions, conjugation, capping, oxidation and deblocking, any of these defects can lead to faulty sequences. The more the accumulation of failed sequences, the lower the possibility of obtaining pure oligonucleotides. This resulted in a mixture of n-1 deletion mutations. BOC RNA's expertise in oligonucleotide synthesis enables us to obtain high-quality DNA long oligonucleotides using the best available synthetic reagents, optimized synthetic protocols, and stringent quality assurance and quality control standards.
For additional information regarding custom long oligonucleotides synthesis, please feel free to contact us.
| Type | Purification | Price | Standard deliverables |
| Long oligonucleotides | PAGE / Dual-PAGE | Inquiry |
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| Ultra-long oligonucleotides | PAGE / Dual-PAGE | Inquiry |
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Long oligonucleotides are essential for gene construction, cloning, and site-directed mutagenesis. They also serve as templates for in vitro transcription and homology-directed repair in gene editing applications.
Ultra-long oligonucleotides (greater than 150 nucleotides) provide more flexibility and accuracy for complex applications such as CRISPR genome editing and targeted resequencing. Their use ensures the generation of large, precise insertions or deletions in genetic sequences.
BOC RNA uses the best available synthetic reagents and optimized protocols to minimize errors in long oligonucleotide synthesis. Every oligonucleotide undergoes stringent quality control and sequencing for confirmation of purity and accuracy.
Long oligonucleotides are widely used in synthetic biology for generating shRNA libraries, SNP positive control templates for qPCR, and other high-complexity gene synthesis applications. They are also critical for genome editing techniques like CRISPR.
BOC RNA purifies long oligonucleotides using PAGE or Dual-PAGE, ensuring high-quality products free of impurities. This method guarantees that the oligonucleotides are suitable for complex applications like gene construction and mutagenesis.
Each long oligonucleotide undergoes rigorous quality control, including sequencing and analysis for sequence accuracy, Tm (melting temperature), and optical density (OD). A Certificate of Analysis (COA) is provided with each order.
