BOC RNA provides linker phosphoramidites used for DNA/RNA synthesis. Use an amino linker to conjugate biotin, fluorescein, or other modifiers and a reporter group to the 5'end of the oligonucleotide or adheres to the surface.
As the DNA-based microarrays and multi-label diagnostic systems become increasingly important, the demand for easy preparation of functionalized oligonucleotides is also growing. At present, the most common modification is the primary amine at the 5'end of the oligonucleotide. TFA-protected and MMT-protected 5'-amino modifiers can be used in automatic synthesizers or manual coupling to connect various moieties to 5'-primary amines, including fluorescent dyes, biotin, and EDTA.
The acid-labile MMT protecting group is stable to basic cleavage and deprotection conditions and can be used as a "handle" for analysis in RP high-performance liquid chromatography to easily separate oligonucleotides from failed sequences. The MMT group is then removed using acidic conditions.
The MMT linker is the best choice when purification by reversed-phase technique is required.
In the cleavage and deprotection steps, the alkali-labile TFA-group can be easily removed with concentrated ammonia. In the case of directly separating amino-modified oligonucleotides from cleaved and deprotected oligonucleotides, base-labile TFA moieties are used.
Custom manufacturing services are available to help develop or expand phosphoramidite compounds.
If you have any questions or requirements, please feel free to contact us.
| Catalog | CAS Number | Product Name | Molecular Formula | Molecular Weight | Price |
| BRP-02206 | 139747-14-1 | TFA-Pentylaminolinker Phosphoramidite | C16H29F3N3O3P | 399.39 | Inquiry |
| BRP-02207 | 168329-40-6 | PC Linker Phosphoramidite | C39H46N3O7P | 699.78 | Inquiry |
| BRP-02208 | 922522-12-1 | ssH-Linker | C38H53N4O5P | 676.83 | Inquiry |
| BRP-02209 | 133975-85-6 | TFA-Hexylaminolinker Phosphoramidite | C17H31F3N3O3P | 413.42 | Inquiry |
| BRP-02210 | 114616-27-2 | 5'-Amino Modifier | C35H48N3O3P | 589.75 | Inquiry |
Linker phosphoramidites introduce functional groups or reporter molecules, such as biotin or fluorescent dyes, at the 5'-end of oligonucleotides, enabling site-specific labeling or surface conjugation.
MMT linkers are acid-labile and useful for reversed-phase purification and coupling analysis, while TFA linkers are base-labile, allowing deprotection under basic conditions without additional steps.
They allow multiple oligonucleotides to be synthesized in a single solid-phase reaction, require lower reagent amounts, and reduce costs while maintaining controlled labeling density.
MMT deprotection is performed with acidic solutions or acetic acid at room temperature, and coupling efficiency can be monitored colorimetrically, ensuring high-purity modified oligonucleotides.
Yes, both MMT- and TFA-protected amino linkers are compatible with automated or manual oligonucleotide synthesis, allowing flexible incorporation of functional groups for research or diagnostic applications.
Each batch undergoes strict quality checks, including HPLC and 31P NMR analysis, with tightly controlled manufacturing processes to ensure minimal impurities and reproducibility between syntheses.
