Dynamic Polyconjugates (DPC)

Dynamic Polyconjugates (DPC)

A new era in siRNA drug development is now underway, thanks to advances in RNAi technology. With impeccable expertise, BOC Sciences is committed to providing siRNA drug design and in vivo delivery solutions for drug development, solving our clients' research challenges.

About Dynamic Polyconjugates

The ability of siRNAs to silence specific genes has generated significant interest in their use as research tools and therapeutic agents for diseases including cancer, infectious diseases, and metabolic disorders. siRNAs for effective delivery to appropriate target cells in vivo are an important component of these siRNA-based applications. Therefore, a variety of non-viral and viral systems are being developed for the delivery of siRNA to the liver, tumors, and other tissues in vivo. To avoid delivery of siRNA to a large number of non-target cells in the liver, researchers have used maleic anhydride to modify the amine groups of amphiphilic poly (vinyl ether), called PBAVE, to form acid-unstable maleic acid bonds. These bonds are cleaved in the acidic environment of endosomes, exposing the drug's amine and activating its endosomal solubility. The delivery vehicle is named siRNA dynamic multi couple to indicate that the siRNA, shielding agent, and targeting ligand are reversibly coupled to the polymer and that the endosomal solubilization properties of the polymer are triggered by its chemical environment.

Schematic showing the siRNA Dynamic PolyConjugate, its cellular uptake, disassembly in the low pH environment of the endosome, and release of the siRNA into the cytoplasm of the target cell.Fig.1 Schematic showing the siRNA Dynamic PolyConjugate, its cellular uptake, disassembly in the low pH environment of the endosome, and release of the siRNA into the cytoplasm of the target cell. (David B. Rozema, 2007)

The advantages of Dynamic Polyconjugates

  • No hepatotoxicity
  • Excellent tolerance
  • Modular platform system where many different elements can be adapted to enable siRNA delivery for a variety of purposes

Application of Dynamic Polyconjugates

  • Liver delivery
  • Extra-hepatic delivery
  • New subcutaneous format

Our services

  • Platform with custom siRNA synthesis
    1. A variety of different chemical modifications and fluorescent labels
    2. Flexible synthesis scale from 0.015 µmol to > 10 µmol
    3. Quality control by analytical HPLC, mass spectrometry
  • Dynamic Polyconjugates
    1. Synthesis of PBAVE
    2. Synthesis of siRNA polycouples: connecting siRNA and PBAVE polymers with disulfide bonds
    3. siRNA-polymer conjugates modification:
        1. PEG or NAG molecules
        2. Carboxydimethylmaleic anhydride (CDM)
        3. Maleic anhydride derivatives
        4. Reversible modifications
  • Characterization of Dynamic Polyconjugates

Our capabilities

  • Platforms with various types of customized services
  • Multiple design features
  • Reversibly linked cellular receptor ligands
  • User-friendly price and excellent reproducibility
  • Strict QA and QC
  • Rush service is available
  • 1 on 1 customer service

With our extensive experience in RNA-related products and services, we are confident that we can help you overcome any upcoming challenges. Our experts are fully equipped to meet your specific needs. With our services, we hope to streamline and accelerate your drug discovery and disease treatment research efforts. Feel free to contact us!

Reference

  1. Rozema D B.; et al. Dynamic PolyConjugates for targeted in vivo delivery of siRNA to hepatocytes. Proceedings of the National Academy of Sciences of the United States of America. 2007, 104(32): 12982-12987.
* Only for research. Not suitable for any diagnostic or therapeutic use.
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