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The chemical modification of siRNA can significantly improve its stability, transfection efficiency and cytotoxicity, which greatly advances the application of siRNA in gene therapy. Here, BOC Sciences provides you with commonly available ribose-modified siRNAs, as well as customized ribose-modified siRNAs.
Ribose Modifications in siRNA
The modification of ribose in siRNA molecules strongly affects the conformation of the sugar and is one of the most important ways to improve the properties of siRNA and expand its applications. These modifications mainly involve the alteration of hydroxyl groups on the ribose, which are important for the stability, specificity and pharmacokinetic properties of siRNA.
Figure 1. Ribose modifications of siRNA.
Ribose deprotonation catalyzed by first using nucleic acid exonucleases and nucleic acid endonucleases can be followed by replacing the 2'-hydroxyl group of the ribose with a less nucleophilic (less likely to provide electrons) substance, such as 2'-O-methyl, 2'-O-methoxyethyl and 2'-fluorine modifications. As an added benefit, these modifications also affect the conformation of the ribose due to their electron-absorbing properties, an effect that increases the binding affinity of the siRNA double-stranded body. It is important to note that the addition of too many of these modifications can inhibit the dissociation of the siRNA double-stranded body during RISC loading. Ribose modifications are more common than backbone modifications because they don’t affect siRNA recognition by RISC.
Ribose-modified siRNA from BOC Sciences
BOC Sciences offers not only a range of common ribose-modified siRNAs, but also custom ribose-modified siRNA services.
| Modification | Quantity | Purification | Detail | Price |
| 2'-Fluoro-rU | 2 OD-250 OD | HPLC | The fluorine atom was substituted for the hydroxyl group in the ribose 2'-position, enhancing the nuclease resistance and potency of siRNA. | Inquiry |
| 2'-Fluoro-rC | 2 OD-250 OD | HPLC | Inquiry | |
| 2'-Fluoro-MOE | 2 OD-250 OD | HPLC | Inquiry | |
| 2'-Fluoro-2'-dA | 2 OD-250 OD | HPLC | Inquiry | |
| 2'-OMe-rU | 2 OD-250 OD | HPLC | The addition of a methyl group at the 2'-position of the ribose enhances the stability of siRNA and reduces off-target effects. | Inquiry |
| 2'-OMe-3'-dA | 2 OD-250 OD | HPLC | Inquiry | |
| 2'-OMe-rC | 2 OD-250 OD | HPLC | Inquiry | |
| 2'-OMe-rA | 2 OD-250 OD | HPLC | Inquiry | |
| 2'-MOE | 2 OD-250 OD | HPLC | The use of methoxyethyl in place of the hydroxyl group at the 2'-position of the ribose improves the potency, stability and resistance to nuclease degradation of siRNA. | Inquiry |
| 2'-Pyrenemethyl | 2 OD-250 OD | HPLC | The addition of a pyrene group at the 2'-position of ribose enables visualization and tracking of siRNA in cells and tissues. | Inquiry |
| 2'-AE | 2 OD-250 OD | HPLC | The introduction of an aminoethyl group at the 2'-position of ribose enhanced cellular uptake of siRNA reducing the off-target effect. | Inquiry |
| 2',4'-BNA | 2 OD-250 OD | HPLC | A methylene bridge is introduced between the 2'-oxygen and 4'-carbon of the ribose, locking the conformation of the ribose and enhancing the binding affinity, specificity and stability of the siRNA. | Inquiry |
| 2'-SATM | 2 OD-250 OD | HPLC | The substitution of 2'-hydroxyl group of the ribose sugar with an allylthiomethyl group enhance the resistance of siRNA to nucleases. | Inquiry |
| 2'-Isoproply | 2 OD-250 OD | HPLC | In 2'-Isopropyl siRNA, the 2'-hydroxyl group on the ribose ring is replaced by an isopropyl group, which can enhance the stability and improve the cell permeability of siRNA. | Inquiry |
BOC Sciences has a team of experts with a decade of experience in RNA synthesis and state-of-the-art facilities to ensure high quality and competitive prices for ribose-modified siRNA. For more custom ribose-modified siRNA, please feel free to contact us.
* Only for research. Not suitable for any diagnostic or therapeutic use.
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