Inquiry Basket
Services Details
The chemical modification of siRNA can significantly improve its stability, transfection efficiency and cytotoxicity, which greatly advances the application of siRNA in gene therapy. Here, BOC Sciences provides you with commonly available ribose-modified siRNAs, as well as customized ribose-modified siRNAs.
Ribose Modifications in siRNA
The modification of ribose in siRNA molecules strongly affects the conformation of the sugar and is one of the most important ways to improve the properties of siRNA and expand its applications. These modifications mainly involve the alteration of hydroxyl groups on the ribose, which are important for the stability, specificity and pharmacokinetic properties of siRNA.
Figure 1. Ribose modifications of siRNA.
Ribose deprotonation catalyzed by first using nucleic acid exonucleases and nucleic acid endonucleases can be followed by replacing the 2'-hydroxyl group of the ribose with a less nucleophilic (less likely to provide electrons) substance, such as 2'-O-methyl, 2'-O-methoxyethyl and 2'-fluorine modifications. As an added benefit, these modifications also affect the conformation of the ribose due to their electron-absorbing properties, an effect that increases the binding affinity of the siRNA double-stranded body. It is important to note that the addition of too many of these modifications can inhibit the dissociation of the siRNA double-stranded body during RISC loading. Ribose modifications are more common than backbone modifications because they don’t affect siRNA recognition by RISC.
Ribose-modified siRNA from BOC Sciences
BOC Sciences offers not only a range of common ribose-modified siRNAs, but also custom ribose-modified siRNA services.
| Modification | Quantity | Purification | Detail | Price |
| 2'-Fluoro-rU | 2 OD-250 OD | HPLC | The fluorine atom was substituted for the hydroxyl group in the ribose 2'-position, enhancing the nuclease resistance and potency of siRNA. | Inquiry |
| 2'-Fluoro-rC | 2 OD-250 OD | HPLC | Inquiry | |
| 2'-Fluoro-MOE | 2 OD-250 OD | HPLC | Inquiry | |
| 2'-Fluoro-2'-dA | 2 OD-250 OD | HPLC | Inquiry | |
| 2'-OMe-rU | 2 OD-250 OD | HPLC | The addition of a methyl group at the 2'-position of the ribose enhances the stability of siRNA and reduces off-target effects. | Inquiry |
| 2'-OMe-3'-dA | 2 OD-250 OD | HPLC | Inquiry | |
| 2'-OMe-rC | 2 OD-250 OD | HPLC | Inquiry | |
| 2'-OMe-rA | 2 OD-250 OD | HPLC | Inquiry | |
| 2'-MOE | 2 OD-250 OD | HPLC | The use of methoxyethyl in place of the hydroxyl group at the 2'-position of the ribose improves the potency, stability and resistance to nuclease degradation of siRNA. | Inquiry |
| 2'-Pyrenemethyl | 2 OD-250 OD | HPLC | The addition of a pyrene group at the 2'-position of ribose enables visualization and tracking of siRNA in cells and tissues. | Inquiry |
| 2'-AE | 2 OD-250 OD | HPLC | The introduction of an aminoethyl group at the 2'-position of ribose enhanced cellular uptake of siRNA reducing the off-target effect. | Inquiry |
| 2',4'-BNA | 2 OD-250 OD | HPLC | A methylene bridge is introduced between the 2'-oxygen and 4'-carbon of the ribose, locking the conformation of the ribose and enhancing the binding affinity, specificity and stability of the siRNA. | Inquiry |
| 2'-SATM | 2 OD-250 OD | HPLC | The substitution of 2'-hydroxyl group of the ribose sugar with an allylthiomethyl group enhance the resistance of siRNA to nucleases. | Inquiry |
| 2'-Isoproply | 2 OD-250 OD | HPLC | In 2'-Isopropyl siRNA, the 2'-hydroxyl group on the ribose ring is replaced by an isopropyl group, which can enhance the stability and improve the cell permeability of siRNA. | Inquiry |
BOC Sciences has a team of experts with a decade of experience in RNA synthesis and state-of-the-art facilities to ensure high quality and competitive prices for ribose-modified siRNA. For more custom ribose-modified siRNA, please feel free to contact us.
* Only for research. Not suitable for any diagnostic or therapeutic use.
Online Inquiry
Services
Products
Highlighted Services
GMP Oligonucleotide Manufacturing Service
For more than 15 years, BOC Sciences has been manufacturing oligonucleotides for pre-clinical, pharmaceutical, food safety, and animal health industries. We provide customized and flexible oligonucleotide GMP or non-GMP production services to meet different production needs.
Lipid Nanoparticle(LNP) for RNA Delivery
BOC Sciences offers comprehensive LNP- mRNA delivery services tailored to meet the specific needs of mRNA vaccine development. Our expertise in nanoparticle formulation and mRNA chemistry enables us to design custom LNP formulations optimized for stability, efficacy, and safety.
BOC Sciences promises to offer you with GalNAc-siRNA conjugation services to help you conduct further research on GalNAc-siRNA conjugates and explore their mores omnics capabilities, the working mechanism as well as their potential therapeutic profiles.
BOC Sciences offers aptamer customization services to generate high-quality aptamers tailored to your goals, delivering excellent results even for the most difficult target molecules.
Peptide-Oligonucleotide Conjugation
BOC Sciences is committed to providing our customers with comprehensive modification and labeling, offering affordable custom oligonucleotides or peptide-oligonucleotide conjugates.
