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In Vitro Transcription Service
BOC Sciences provides one-stop in vitro transcription services, from template synthesis, in vitro RNA transcription, product QC to packaging, with rich experience. BOC Sciences relies on self-developed technology and continuously optimized process to provide you with in vitro RNA transcription services with high purity and low impurity.
What is In Vitro Transcription?
In vitro transcription is the process of transcribing a DNA template into an RNA molecule under in vitro conditions using a nucleic acid polymerase (e.g., RNA polymerase). This method is commonly used to synthesize large quantities of RNA molecules for various experiments and applications, such as gene expression studies, RNA structure analysis, and drug screening.
Fig 1. Mechanism of in vitro transcription. (Hu et al., 2021)
BOC Sciences' In Vitro Transcription Services
BOC Sciences provides professional in vitro RNA transcription services and performs both small-scale and large-scale transcription production.
| Service Item | Specifications |
| Transcription Template Synthesis | Based on the RNA sequence to be synthesized, design a DNA sequence that is complementary to it. Computer software or online tools can be used to design the desired DNA sequence. The synthesized DNA is then identified and sequenced to confirm that the desired DNA sequence is correct. This process is accomplished using DNA sequencing technologies such as Sanger sequencing or next-generation sequencing methods. |
| Template Processing Services | If the DNA template is circular (e.g. plasmid DNA), it needs to be linearized and a T7 promoter added to the sequence so that RNA polymerase can transcribe on it. |
| In Vitro Transcription Services | A DNA template for the desired synthesized RNA, RNA polymerase, and appropriate nucleotides (ATP, CTP, GTP, and UTP) are added to the reaction system for in vitro transcription. |
| Purification & Quality Control | RNA purification can be performed by column chromatography, enzyme treatment, gel electrophoresis, etc. RNA quality control can be performed by real-time fluorescent quantitative PCR (qPCR), spectrophotometer, RNA quality assessment instruments, etc. |
Transcription Requirements
In vitro transcription reactions require purified promoter-containing linear DNA templates, ribonucleotide triphosphate, buffer systems containing DTT and magnesium ions, and appropriate amounts of phage RNA polymerase. The specific conditions required for the transcription reaction depend on the amount of RNA needed for the particular application.
Deliverables
- RNA product of transcription (typically not less than 80 μg RNA for one transcription)
- Experimental report (experimental steps and RNA quality control results)
Applications of In Vitro Transcription
RNA Functional Studies
In vitro transcribed RNA molecules can be used to study the function and activity of specific RNA sequences or RNA-binding proteins.
RNA Structure and Folding Studies
By labeling or modifying RNA transcripts during synthesis, researchers can use techniques such as chemical probing, RNA footprinting, and structure prediction to probe and study RNA secondary structures, tertiary interactions, and RNA-protein interactions.
RNA Libraries and Screening
In vitro transcription allows the construction of a variety of RNA libraries, such as aptamer libraries or RNA interference (RNAi) libraries.
Based on BOC Sciences' extensive technology and experience, we can provide you with high-quality products and services. If you are interested in our services, please feel free to contact us.
Reference
- Hu Y, et al. A strategy for programming the regulation of in vitro transcription with application in molecular circuits[J]. Nanoscale, 2021, 13(10): 5429-5434.
* Only for research. Not suitable for any diagnostic or therapeutic use.
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