In Vitro Transcription Service

BOC Sciences provides one-stop in vitro transcription services, from template synthesis, in vitro RNA transcription, product QC to packaging, with rich experience. BOC Sciences relies on self-developed technology and continuously optimized process to provide you with in vitro RNA transcription services with high purity and low impurity.

What is In Vitro Transcription?

In vitro transcription is the process of transcribing a DNA template into an RNA molecule under in vitro conditions using a nucleic acid polymerase (e.g., RNA polymerase). This method is commonly used to synthesize large quantities of RNA molecules for various experiments and applications, such as gene expression studies, RNA structure analysis, and drug screening.

Fig 1. Mechanism of in vitro transcription. (Hu et al., 2021)

BOC Sciences' In Vitro Transcription Services

BOC Sciences provides professional in vitro RNA transcription services and performs both small-scale and large-scale transcription production.

• Transcription Requirements

In vitro transcription reactions require purified promoter-containing linear DNA templates, ribonucleotide triphosphate, buffer systems containing DTT and magnesium ions, and appropriate amounts of phage RNA polymerase. The specific conditions required for the transcription reaction depend on the amount of RNA needed for the particular application.

• Deliverables

• RNA product of transcription (typically not less than 80 μg RNA for one transcription)
• Experimental report (experimental steps and RNA quality control results)

Applications of In Vitro Transcription

• RNA Functional Studies

In vitro transcribed RNA molecules can be used to study the function and activity of specific RNA sequences or RNA-binding proteins.

• RNA Structure and Folding Studies

By labeling or modifying RNA transcripts during synthesis, researchers can use techniques such as chemical probing, RNA footprinting, and structure prediction to probe and study RNA secondary structures, tertiary interactions, and RNA-protein interactions.

• RNA Libraries and Screening

In vitro transcription allows the construction of a variety of RNA libraries, such as aptamer libraries or RNA interference (RNAi) libraries.

Based on BOC Sciences' extensive technology and experience, we can provide you with high-quality products and services. If you are interested in our services, please feel free to contact us.

Reference

1. Hu Y, et al. A strategy for programming the regulation of in vitro transcription with application in molecular circuits[J]. Nanoscale, 2021, 13(10): 5429-5434.

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