Small interfering RNAs (siRNAs) have emerged as powerful tools for gene silencing, enabling researchers to dissect gene function, validate therapeutic targets, and develop next-generation RNA-based therapies. Precision in siRNA design, synthesis, and characterization is essential to ensure specificity, stability, and effective knockdown efficiency. At BOC Sciences, we provide end-to-end siRNA services that integrate advanced design algorithms, high-quality synthesis, and rigorous quality control, supporting both academic research and pharmaceutical development.
The use of siRNA in functional genomics and therapeutic research is often hindered by technical and biological challenges. Selecting the right partner to provide robust siRNA services ensures that these hurdles are addressed effectively.
– Poorly designed siRNAs often result in inconsistent or irreproducible knockdown, wasting valuable research resources. Our custom design platforms integrate advanced predictive algorithms to minimize these risks.
– Sequence-dependent artifacts and unintended gene modulation compromise data integrity. Our optimization strategies enhance specificity while reducing unwanted interactions.
– Native siRNAs are prone to degradation and poor cellular uptake. With customized chemical modifications and conjugation strategies, we enhance siRNA half-life and delivery performance.
– Reliable siRNA synthesis and screening services provide validated data critical for target discovery, pathway studies, and translational research.
– High-throughput siRNA libraries and screening platforms shorten timelines and maximize the identification of functional targets.
By addressing these core obstacles, BOC Sciences ensures that clients achieve reproducible, high-confidence results that stand up to rigorous scientific and regulatory scrutiny.
Get A QuoteAt BOC Sciences, we understand that no two research projects are identical. Every gene target, disease model, or discovery pipeline comes with its own complexities. To meet these diverse demands, we have built a comprehensive siRNA service portfolio that spans from basic custom design to highly advanced, specialized solutions. Our portfolio is designed to be both modular and scalable, giving clients the flexibility to select individual services or integrate them into a complete RNAi workflow. By combining expertise in oligonucleotide chemistry with cutting-edge bioinformatics, we ensure that our siRNA services deliver not only technical precision but also meaningful biological outcomes.
Effective RNAi research begins with intelligent sequence design. Our siRNA design services integrate advanced bioinformatics algorithms, structural accessibility prediction, and off-target filtering to create highly specific and potent siRNA candidates. By analyzing target mRNA structure, thermodynamics, and GC content, we optimize silencing efficiency while minimizing unwanted interactions. This service is particularly valuable for researchers who want to ensure experimental reproducibility and eliminate costly trial-and-error associated with poorly designed sequences.
The success of RNAi experiments hinges on the design of highly specific and potent siRNA sequences. Our custom siRNA service leverages advanced algorithms and in-depth sequence analysis to create siRNAs optimized for silencing efficiency, specificity, and reproducibility. Each sequence is synthesized under stringent quality standards, ensuring consistent results across replicates and experiments. This service is particularly suited for target validation studies, functional genomics, and preclinical discovery research.
For projects requiring enhanced performance, we provide custom chimera siRNAs—engineered hybrids that combine structural diversity and chemical modifications. These siRNAs are crafted to overcome limitations of traditional designs, offering improved nuclease resistance, enhanced binding affinity, and superior transfection efficiency. Chimera siRNAs are especially advantageous in complex biological systems or preclinical models, where stability and potency are critical.
Our siRNA libraries allow researchers to accelerate discovery by accessing broad collections of validated siRNAs targeting specific pathways, disease-related genes, or entire genomes. By reducing the time and resources required to develop individual sequences, these libraries enable efficient high-throughput functional screening and gene network analysis. Whether used for oncology, neuroscience, or infectious disease research, they provide a powerful starting point for large-scale investigations.
To further empower discovery efforts, we offer siRNA screening services that integrate automated platforms, high-content readouts, and data analytics. This service enables researchers to evaluate large panels of siRNAs rapidly, identifying the most effective candidates for gene silencing. With expert data interpretation and optimized workflows, our screening solutions help clients minimize false positives and negatives, speeding up the transition from raw data to actionable insights.
To address challenges in delivery and stability, BOC Sciences offers a wide range of siRNA conjugates. By covalently attaching siRNAs to functional groups—such as lipids, peptides, antibodies, or nanoparticles—we enhance cellular uptake, tissue targeting, and in vivo half-life. These conjugates are especially powerful in preclinical models, where efficient delivery and controlled biodistribution are critical. Our customizable conjugation strategies provide researchers with versatile tools to bridge the gap between in vitro silencing efficiency and translational research success.
One of the most common challenges in siRNA applications is limited stability and poor in vivo performance. Our custom chemically modified siRNAs address this issue directly. By incorporating modifications such as 2′-O-methyl, 2′-fluoro, locked nucleic acids (LNAs), or phosphorothioate backbones, we enhance nuclease resistance, reduce immunogenic responses, and improve pharmacokinetic properties. These modifications make our siRNAs ideal for demanding preclinical studies that require prolonged activity and minimal off-target effects.
Understanding cellular uptake and intracellular dynamics is critical in RNAi research. With fluorescence-labeled siRNAs, researchers can track the delivery and localization of their molecules in real time. These siRNAs, tagged with dyes such as FAM, Cy3, or Cy5, are invaluable for studying transfection efficiency, endosomal escape, and subcellular trafficking, offering insights that improve both basic research and therapeutic development.
Reliable gene silencing studies depend on the proper use of controls. We provide a full range of siRNA controls, including scrambled siRNAs, mismatch controls, positive controls targeting housekeeping genes, and negative controls. These validated controls help researchers confirm sequence-specific effects, eliminate experimental artifacts, and ensure robust interpretation of results.
To eliminate single-sequence variability, our siRNA sets contain multiple siRNAs targeting the same gene. This strategy increases the probability of achieving consistent and potent knockdown while reducing the influence of off-target effects. By using siRNA sets, researchers can strengthen data reliability, confirm gene-specific outcomes, and avoid false conclusions driven by suboptimal sequences.
For projects requiring stable, long-term gene silencing, our shRNA plasmid expression vectors provide a durable alternative to synthetic siRNAs. These vectors are designed for continuous expression in mammalian cells, making them suitable for chronic studies and pathway analysis. Available with customizable promoters and selectable markers, they offer flexibility for integration into diverse research systems.
Our RNAi services provide a holistic platform for researchers seeking to harness gene silencing technologies. By bridging computational design, chemical synthesis, and functional validation, we reduce the experimental guesswork that often delays research. This service is ideal for clients who need a fully managed solution—from the selection of effective siRNA sequences to the delivery of validated reagents that are ready for immediate use in downstream assays.
A well-structured workflow is the foundation of successful siRNA projects. At BOC Sciences, we have established a comprehensive, transparent, and highly customizable workflow that ensures efficiency, scientific rigor, and reproducibility at every stage. Our process is designed not only to deliver high-quality siRNAs, but also to support researchers in overcoming the most common barriers in RNAi research, from sequence design to downstream validation.
Every collaboration begins with a thorough consultation to understand the client's research objectives, experimental system, and specific challenges. Our scientific team works closely with researchers to:
This strategic planning step ensures that every subsequent stage is aligned with the client's scientific goals.
Design quality directly determines siRNA performance. We apply state-of-the-art algorithms and predictive models that incorporate:
By combining computational precision with expert curation, we significantly improve the likelihood of successful knockdown.
Once sequences are finalized, synthesis is performed on advanced oligonucleotide platforms under strict quality conditions. Our capabilities include:
This stage ensures that each siRNA molecule meets stringent purity and structural integrity standards.
Every siRNA product undergoes multi-tiered analytical validation, which may include:
These QC procedures guarantee that siRNAs perform consistently across replicates and experiments.
Final products are delivered in lyophilized or solution form, depending on client preference. Each shipment includes comprehensive documentation with:
This ensures transparency and confidence in the reagents received.
Our commitment does not end with delivery. Clients gain access to ongoing technical support to help troubleshoot experiments, optimize transfection conditions, and interpret silencing results. By acting as a collaborative partner rather than just a supplier, we maximize the scientific value of every siRNA project.
Choosing the right partner for siRNA design and synthesis can be the difference between wasted resources and breakthrough discoveries. At BOC Sciences, we deliver more than just oligonucleotides—we provide a strategic research partnership backed by scientific expertise, state-of-the-art technology, and uncompromising quality.
Our team brings decades of collective experience in oligonucleotide synthesis, RNA structural biology, and RNAi technologies. This scientific depth allows us to anticipate common pitfalls—such as off-target effects, poor transfection, or instability—and proactively design solutions that safeguard research outcomes. Unlike generic providers, we combine chemistry know-how with biological insight, ensuring our siRNAs are optimized not only for molecular integrity but also for functional performance in biological systems.
From custom sequence design to specialized modifications and fluorescent labeling, our platform allows complete flexibility. Clients can tailor:
This level of customization ensures that every project receives a solution precisely aligned with its scientific requirements.
High-quality siRNA is non-negotiable for reproducible results. All of our siRNAs undergo stringent analytical validation including HPLC purification, mass spectrometry confirmation, and concentration assessment. Optional functional assays are available to confirm knockdown efficiency. With every delivery, clients receive full Certificates of Analysis (CoA) and supporting QC documentation. This transparency ensures confidence in the reliability and reproducibility of our products.
Our platform is engineered for scalability. Whether a client requires a few milligrams of custom siRNA for proof-of-concept experiments or large-scale siRNA libraries for genome-wide screening, BOC Sciences can deliver without compromising quality. This scalability supports projects across the research spectrum, from small labs to industrial drug discovery pipelines.
The versatility of siRNA technology has transformed it into a cornerstone of modern life sciences. At BOC Sciences, our siRNA services empower researchers across a broad spectrum of disciplines, from functional genomics to preclinical drug discovery. Each application benefits from tailored design, chemical precision, and validated reproducibility, ensuring that experimental outcomes are robust and scientifically meaningful.
siRNAs are indispensable tools for dissecting gene function in cellular and organismal models. By silencing specific targets, researchers can:
Our custom siRNA and siRNA sets ensure that gene silencing is reliable and reproducible, enabling precise functional characterization.
In pharmaceutical R&D, siRNAs are instrumental for validating disease-associated genes before committing to costly therapeutic development. Applications include:
By ensuring efficient and specific knockdown, BOC Sciences accelerates the validation phase, saving both time and resources in early drug discovery pipelines.
siRNAs provide a powerful approach for modeling therapeutic interventions at the preclinical stage. For example:
Our chemically modified siRNAs offer improved stability and reduced off-target effects, making them highly reliable for these demanding studies.
RNAi has proven invaluable in understanding infectious disease biology. Applications include:
BOC Sciences supports these efforts with custom libraries and siRNA screening services, enabling rapid identification of host-pathogen interaction points.
Cancer research increasingly relies on siRNAs to interrogate oncogenes, tumor suppressors, and resistance pathways. Specific uses include:
Our siRNA sets and controls provide the reliability needed for high-stakes oncology research, minimizing artifacts and strengthening translational relevance.
siRNAs are applied in the study of complex diseases such as neurodegeneration and metabolic syndromes. Examples include:
Our fluorescence-labeled siRNAs facilitate live-cell imaging in neuronal cultures, providing spatial and temporal insights into knockdown dynamics.
RNAi-based tools also serve as valuable benchmarks for developing new delivery platforms. Researchers use siRNAs to:
Our customizable siRNA solutions allow side-by-side evaluations, making them indispensable for the advancement of nucleic acid delivery technologies.
By leveraging genome-wide siRNA libraries, researchers can perform large-scale functional screens to map gene interactions at the systems level. Applications include:
Our high-throughput screening platforms combine automated workflows with data-driven analytics, offering unmatched scalability for complex systems biology studies.
RNA interference (RNAi) is a biological process in which RNA molecules inhibit gene expression, first observed in plants and C. elegans, and later in mammalian cells. This advance was recognized as one of the greatest strides in biology and in 2006, Andrew Fire and Craig C. Mello were awarded the Nobel Prize in Physiology or Medicine for their effort in the creation of RNAi technology. Currently, RNAi is emerging as a promising area of cancer therapy. The two effector molecules of the RNAi are small interfering RNA (siRNA) and micro RNA (miRNA). siRNA is a group of small (20-30 nucleotides), double stranded, non-coding single stranded RNA impressed molecules. One of the most crucial levels of genome function that siRNA regulates is gene expression in eukaryotes, an event that entails both estrogen and non estrogen-regulated responses, for example chromosome segregation, chromatin structure, RNA processing, RNA stability, transcription, and translation.
The mechanism of siRNA action is elegantly simple yet remarkably powerful. Once they are brought into the cell, the siRNA molecules are cleaved by an enzyme called Dicer, resulting in short double-stranded fragments. One of them, the guide strand, is assembled into the RNA-induced silencing complex (RISC) and is used as a template for target recognition. Once loaded onto the RNA-induced silencing complex (RISC), the siRNA guides the RISC complex to the matching target mRNA and promotes the degradation of the mRNA, and hence, the gene that it encodes. Yet this mechanism occurs with exquisite specificity, allowing scientists to target the expression of genes associated with a disease without affecting other healthy genes.
Although microRNA (miRNA) and siRNA act within the general pathway of RNA interference, they have unique properties and modes of action. miRNAs are small RNA molecules that are endogenously expressed and encoded in the genome, that act through imperfect base-pairing with their target mRNAs to repress gene expression. In contrast, siRNAs are exogenous RNA molecules that are being re-introduced into the cell to specifically target and silence an individual gene with exact sequence complementarity. This widened targeting specificity makes siRNA particularly well suited for therapeutic purposes where strict regulation of gene expression is desired.
The siRNA should be designed from a unique and well-conserved region of the target mRNA, excluding secondary structures or repeat sequences. The siRNA sequence complements the target mRNA leading to mRNA degradation. Effective gene silencing requires the careful design of CRISPR targeting strategies, taking into account sequence specificity and potential off-target effects.
Small interfering RNA (siRNA) is a class of double-stranded RNA (dsRNA) molecules, either endogenous or created via chemical synthesis, that play a crucial role in the gene expression processes of an organism by systematically downregulating or silencing the activity of a gene. Dicer, an endoribonuclease, cleaves the dsRNA molecules into short sections of 20–25 bp called the siRNAs.
The binding of siRNA with its complementary mRNA silences gene expression. This binding in turn, recruits the RNA-induced silencing complex (RISC), which cleaves the mRNA so that it cannot then be translated into protein. This system thereby silences a gene in a manner that is all-or-nothing.
siRNA vs shRNA differ both in their structure and delivery of design. Though siRNA is just a small piece of double-stranded RNA that can be introduced directly into cells as a naked two-stranded piece, shRNA is a larger piece of RNA, containing a hairpin loop, in one way or another introduced by plasmids or viral vectors that will be processed into siRNA inside the cell. They are both meant to silence genes.
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