5'-O-DMT-2'-O-TBDMS-N2-isobutyryl-L-Guanosine 3'-CE phosphoramidite - CAS 679809-76-8
Catalog number: BRA-038
5'-O-DMT-2'-O-TBDMS-N2-isobutyryl-L-Guanosine 3'-CE phosphoramidite, a sophisticated compound integral to the production of custom oligonucleotides, showcases remarkable versatility. It serves as a phosphoramidite foundation during solid-phase DNA synthesis, facilitating the integration of guanosine modifications. By harnessing this product, nucleic acid researchers can probe gene expression or delve into the impact of guanosine alterations on ailments such as cancer.
* Please kindly note that our products are not to be used for therapeutic purposes and cannot be sold to patients.
1. A universal, photocleavable DNA base: nitropiperonyl 2'-deoxyriboside
M C Pirrung, X Zhao, S V Harris. J Org Chem. 2001 Mar 23;66(6):2067-71. doi: 10.1021/jo001594r.
A universal, photochemically cleavable DNA base analogue would add desirable versatility to a number of methods in molecular biology. A novel C-nucleoside, nitropiperonyl deoxyriboside (NPdR, P), has been investigated for this purpose. NPdR can be converted to its 5'-DMTr-3'-CE-phosphoramidite and was incorporated into pentacosanucleotides by conventional synthesis techniques. The destabilizing effect on hybrid formation with a complementary strand when this P base opposes A, T, and G was found to be 3-5 kcal/mol, but 9 kcal/mol when it opposes C. Brief irradiation (lambda > 360 nm, 20 min) of DNA containing the P base and piperidine treatment causes strand cleavage giving the 3'- and 5'-phosphates. Two significant recent interests, universal/non-hydrogen-bonding base analogues and photochemical backbone cleavage, have thus been combined in a single molecule that serves as a light-based DNA scissors.