Oligonucleotides are biopolymers that are sensitive to a variety of environmental factors such as radiation, temperature, water, salt, oxidation, and pH. And their stability is one of the key determinants of a drug's safety and efficacy. BOC Sciences offers oligonucleotide stability testing services that provide stability studies for every stage of development.
Because oligonucleotides are susceptible to external conditions or their own chemical properties, they can become unstable. For example, oligonucleotides are subject to degradation by nuclease enzymes and thus lose their activity. Under alkaline conditions, oligonucleotides are also subject to hydrolysis, resulting in chain breakage and base loss. Furthermore, base damage can also occur if exposed to ultraviolet light. In addition to the above factors, they may also be affected by the environment, such as temperature, humidity and oxygen, or their own sequence and structure may allow degradation to occur within themselves. Therefore, it is important to analyze the stability of oligonucleotides so that oligonucleotide design, synthesis, and stocking protocols can be modified based on this analysis.
BOC Sciences provides oligonucleotide stability analysis, including pyrolysis, enzymatic degradation, and alkaline hydrolysis, which is performed under external conditions and by its own properties.
BOC Sciences can provide pyrolysis analysis to evaluate the stability of oligonucleotides by measuring the change in absorbance at different temperatures.
Enzyme degradation test requires the use of nuclease, which reacts with the oligonucleotide and then analyzes the degradation of the oligonucleotide by gel electrophoresis or high-performance liquid chromatography.
Oligonucleotides are unstable in alkaline environments, as they need to be placed in an alkaline solution and analyzed by appropriate analytical methods when performing stability assessment.
In addition to providing stability analysis of oligonucleotides, BOC Sciences can also provide solutions to improve oligonucleotide stability. The following are the ways to improve stability provided by our researchers.
Due to the nature of the oligonucleotide's own existence, the backbone can be chemically modified without altering its own ability, thereby enhancing resistance to enzymatic degradation and temperature.
(2) Addition of Nuclease Inhibitors
During the application or storage of oligonucleotides, nuclease inhibitors can be added to inhibit enzyme activity.
(3) Appropriate Storage Conditions
Oligonucleotides need to be stored in a dry, frozen environment according to their suitable storage environment to avoid hydrolysis of oligonucleotides.
(4) Increase Oligonucleotide Concentration
Higher oligonucleotide concentration can reduce the effect of degradation. In experiments or applications, consider using higher concentrations of oligonucleotides to improve their stability.
Our team of analytical experts has developed, implemented, optimized and validated hundreds of sequences and structure-specific high-resolution methods. At BOC Sciences, we can provide our customers with high-quality oligonucleotide products along with comprehensive analytical services for oligonucleotide. If you are interested in our services, please feel free to contact us.