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Custom PCR Primer Synthesis

BOC Sciences offers flexible primer design and custom synthesis options to fit your experimental needs. We monitor the synthesis of each primer and deliver them to each customer for direct application in a variety of downstream experiments.

What is PCR Primer?

PCR primers are synthetic DNA oligonucleotides containing 15-30 bases that bind to the flanking sequences of the target region in the template DNA (through sequence complementation). During the PCR reaction, DNA polymerase extends the primer from the 3' end. Therefore, the primer binding site must be unique to the vicinity of the target and have minimal sequence homology to the rest of the starting DNA to ensure specific amplification of the target fragment.

Solid-phase CuAAC modification of oligonucleotides.Fig 1. Schematic of primer design. (Ogren et al., 2019)

PCR Primer Synthesis Service

PCR primer synthesis and design can usually be found in primer libraries, or custom synthesized according to the needs of low-cost, easy to operate, etc. PCR primer synthesis can be used for sequencing, whole gene synthesis, subcloning, point mutation, vector construction, RNA interference and recombination, and other experiments.

Purification MethodDescription
RPC PurificationRPC purification is the purification of PCR primers by Reverse Phase Cartridge, and the purification principle is the same as reverse phase HPLC purification.
ePAGE PurificationePAGE is a method of removing short sequences and impurities from products by specific adsorption of whole sequences. ePAGE is a more efficient method of DNA purification with automatic batch sampling and purification equipment.
PAGE PurificationThe PAGE purification method uses denaturing polyacrylamide gel electrophoresis to separate the primer DNA and then recover the target DNA from the gel.
HPLC PurificationHPLC purification is the purification of primed DNA using the principles of high-performance liquid chromatography. This method achieves high purity and sensitivity when used for isolation and purification or analysis.

Advantages of BOC Sciences' PCR Primer Synthesis

Reference

  1. Ogren A, et al. Snord94 expression level alters methylation at C62 in snRNA U6[J]. Plos one, 2019, 14(12): e0226035.
* Only for research. Not suitable for any diagnostic or therapeutic use.
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