As an industry leader in high-quality oligonucleotide synthesis and formulation services, BOC Sciences is committed to using proprietary technology to produce high-quality dsRNA oligonucleotides to meet customers' individual needs.
Double-stranded RNA (dsRNA) is a unique form of RNA. dsRNAs are usually shorter, possess enhanced structural instability due to uncertainties, and are highly influenced by environments. Thus, there is no fixed and defined secondary structure for dsRNA.
dsRNAs are usually found within viruses and reserved two main characteristics:
dsRNA is not only a biomarker of viral infection but also a key molecule for RNA interference (RNAi)-mediated gene silencing at the mRNA level.
dsRNA can be constructed in vitro and transferred into cells via liposomes. However, cells with weak liposomal delivery systems tend to result in shorter dsRNAs half-life. Thus, transferring vectors that are capable of expressing dsRNA in vitro can enhance effective transfection and enable dsRNA to play a long-term role in blocking genes to express vectors over a long period stably.
Pol III is used to direct RNA synthesis during dsRNA expression vectors construction, and T7 is used as a promoter to synthesize dsRNA.
| Services | Custom dsRNA synthesis | |
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| Sequence Length | All ssRNAs are available from a few dozens up to several thousand bases | |
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| Purification | Desalted, deprotected, PAGE, HPLC, and/or MS | |
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dsRNA consists of two complementary RNA strands, while ssRNA contains only one strand. dsRNA is key for RNA interference (RNAi) and gene silencing, while ssRNA is primarily involved in mRNA processes.
dsRNA is used for RNAi-based gene silencing, plant protection, and crop improvement. It also plays a significant role in diagnostic applications and viral model systems.
BOC Sciences uses RNA polymerase for in vitro synthesis of dsRNA, providing custom sequences and lengths. Our synthesis process is highly controlled to ensure high purity and sequence accuracy.
We offer a wide range of modifications for dsRNA, including phosphorothioates, spacers, biotin labeling, and fluorophores. These modifications enhance stability and functionality for various research needs.
Each batch of dsRNA is verified using mass spectrometry and undergoes strict quality control for purity and sequence accuracy. We provide Certificates of Analysis (COA) and QC reports for all synthesized products.
Turnaround times for dsRNA synthesis typically range from a few days to a few weeks, depending on the complexity of the project. We also offer expedited services to meet urgent research needs.
