Short Oligo Synthesis

What are Short Oligo?

Short oligo are small nucleotide chains typically 20-30 nucleotides in length. Short oligos are synthesized using a solid-phase synthesis method in which the nucleotides are added sequentially to a solid support, with each addition protected by a chemical group to prevent unwanted reactions. The protected oligonucleotides are then cleaved from the support and deprotected to produce the final product. The efficiency and quality of the synthesis depends on many factors, including the choice of solid support, coupling reagents, and deprotection conditions.

BOC Sciences offers a wide range of oligonucleotide synthesis options, and we also offer oligo pool synthesis for customers with large-scale oligonucleotide programs. With extensive experience in the field of synthetic biology, BOC Sciences is a trusted partner for all your synthetic biology needs.

Synthesis of short oligoFig 1. Synthesis of short oligo. (Jadhav et al., 2012)

BOC Sciences' Short Oligo Synthesis Services

BOC Sciences offers a range of short oligonucleotide synthesis development services to meet the specific needs of our customers. Our team of experienced scientists work closely with our customers to optimize the synthesis process and ensure high-quality results. We offer a variety of solid supports, coupling reagents and deprotection conditions to meet different applications and requirements.

  • Short Oligo Preparation Procedure

The experimental process for short oligonucleotide synthesis typically involves the following steps:

a) Design - The sequence of the oligonucleotide is designed according to the specific application and requirements.

b) Synthesis - Oligonucleotides are synthesized using solid-phase synthesis methods, where each nucleotide is added sequentially to a solid-phase support and protected with a chemical moiety.

c) Cleavage - The oligonucleotides are cleaved from the solid phase support and deprotected to obtain the final product.

d) Purification - Oligonucleotides are purified by HPLC or other methods to remove impurities and ensure high purity.

e) Analysis - The purity, identity and quality of the oligonucleotides are confirmed using analytical methods such as HPLC and mass spectrometry.

BOC Sciences’ Advantages of Short Oligo Synthesis

Synthesis of short oligonucleotides can be challenging due to the difficulty in obtaining high yields and purity. However, BOC Sciences has developed many strategies to overcome these challenges and produce high-quality oligonucleotides. The following are the advantages of short oligo synthesis preparation.

  • High Purity - Our oligonucleotides are typically >95% pure as determined by HPLC analysis.
  • High Yield - We typically achieve >90% yield for oligonucleotides up to 30 nucleotides in length.
  • Fast Turnaround - We can typically synthesize and deliver oligonucleotides within 2-3 weeks.
  • Customization - We can synthesize oligonucleotides with specific modifications, such as fluorescent labeling or phosphorothioate bonds.

BOC Sciences, as a supplier of products and services for oligonucleotides, can customize professional solutions for our clients. If you are interested in our services, please feel free to contact us.


  1. Jadhav A H, Kim H. Short oligo (ethylene glycol) functionalized imidazolium dicationic room temperature ionic liquids: Synthesis, properties, and catalytic activity in azidation[J]. Chemical engineering journal, 2012, 200: 264-274.
* Only for research. Not suitable for any diagnostic or therapeutic use.
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