Short oligo are small nucleotide chains typically 20-30 nucleotides in length. Short oligos are synthesized using a solid-phase synthesis method in which the nucleotides are added sequentially to a solid support, with each addition protected by a chemical group to prevent unwanted reactions. The protected oligonucleotides are then cleaved from the support and deprotected to produce the final product. The efficiency and quality of the synthesis depends on many factors, including the choice of solid support, coupling reagents, and deprotection conditions.
BOC Sciences offers a wide range of oligonucleotide synthesis options, and we also offer oligo pool synthesis for customers with large-scale oligonucleotide programs. With extensive experience in the field of synthetic biology, BOC Sciences is a trusted partner for all your synthetic biology needs.
Fig 1. Synthesis of short oligo. (Jadhav et al., 2012)
BOC Sciences offers a range of short oligonucleotide synthesis development services to meet the specific needs of our customers. Our team of experienced scientists work closely with our customers to optimize the synthesis process and ensure high-quality results. We offer a variety of solid supports, coupling reagents and deprotection conditions to meet different applications and requirements.
The experimental process for short oligonucleotide synthesis typically involves the following steps:
a) Design - The sequence of the oligonucleotide is designed according to the specific application and requirements.
b) Synthesis - Oligonucleotides are synthesized using solid-phase synthesis methods, where each nucleotide is added sequentially to a solid-phase support and protected with a chemical moiety.
c) Cleavage - The oligonucleotides are cleaved from the solid phase support and deprotected to obtain the final product.
d) Purification - Oligonucleotides are purified by HPLC or other methods to remove impurities and ensure high purity.
e) Analysis - The purity, identity and quality of the oligonucleotides are confirmed using analytical methods such as HPLC and mass spectrometry.
Synthesis of short oligonucleotides can be challenging due to the difficulty in obtaining high yields and purity. However, BOC Sciences has developed many strategies to overcome these challenges and produce high-quality oligonucleotides. The following are the advantages of short oligo synthesis preparation.
BOC Sciences, as a supplier of products and services for oligonucleotides, can customize professional solutions for our clients. If you are interested in our services, please feel free to contact us.