Tel:
Email:

What are Sensitive DNA/RNA Synthesis Materials?

DNA/RNA can co-exist with many sensitive organic functional groups. Examples of such groups include alkyl halides, benzyl halides, allyl halides, α-halo amides, esters, activated esters, carbonates, thioesters, tosylates, sulfonic esters, sultones, phosphates, α, β-unsaturated carbonyls, epoxides, aziridines, maleimides, vinyl arenes and methides. However, DNA/RNA analogs containing these groups are not accessible using technologies on the market. The reason is that those technologies use acyl groups for amino protection, 2-cyanoethyl groups for phosphate protection, and ester groups as cleavable linker. These protecting groups and linkers have to be deprotected and cleaved under harsh conditions, under which, the sensitive groups cannot survive.

A newly reported technology using the Dim-Dmoc phosphoramidites 1a-d and Dmoc supports 2a-d which is developed by Shiyue Fang's group can be used for sensitive DNA/RNA synthesis. The DNA/RNA synthesized with these materials can be represented with 3. Deprotection and cleavage can be achieved under mild conditions at pH 4-8 using a 2-step protocol to give the intermediate 4 and then the fully deprotected DNA/RNA 5. Therefore, the new technology is universally useful for the synthesis of DNA/RNA containing a wide range of sensitive groups. It has been demonstrated that DNA/RNA containing the sensitive alkyl esters, phenyl esters, thioesters, alkyl halides, α-halo amides and chloropurine as represented by 6a-f can be readily synthesized using the technology. Analogs containing the other aforementioned sensitive groups as represented by 6g-k are expected to be accessible as well. Importantly, this technology can also be extended fore for the synthesis of sensitive RNA analogs as represented by 6l-o.

Sensitive DNA/RNA Synthesis

Sensitive DNA/RNA Synthesis

Sensitive DNA/RNA Synthesis

Sensitive DNA/RNA analogs accessible with the Dim-Dmoc technology can find applications in four areas. One area is the development of DNA/RNA cross-linking agents for antisense drug and DNA/RNA-based disease diagnosis applications. The other is the study of protein interactions. The third area is the study of origin, function, fate, disease association and other aspects of naturally occurring sensitive DNA/RNA analogs. The fourth area is the use of mRNA to generate protein in vivo as medicines.

We can provide the Dim-Dmoc phosphoramidites and Dmoc supports for your sensitive DNA/RNA synthesis. Here are some sensitive noncanonical nucleosides which can be synthesized using this technology.

Ref:

  1. Shahien Shahsavari, Dhananjani N. A. M. Eriyagama, Jinsen Chen, Bhaskar Halami, Yipeng Yin, Komal Chillar, and Shiyue Fang. The Journal of Organic Chemistry. 2019 84 (21), 13374-13383.

Related Products

CatalogCAS NumberProduct NameMolecular FormulaMolecular WeightPrice
BRP-0212220244-86-47-MethylguanosineC11H16N5O5298.28Inquiry
BRP-02123113886-71-8N4-Acetyl-2'-O-methylcytidineC12H17N3O6299.28Inquiry
BRP-0212424719-82-2N6-ThreonylcarbamoyladenosineC15H20N6O8412.36Inquiry
BRP-021253768-18-1N4-AcetylcytidineC11H15N3O6285.25Inquiry
BRP-02126107865-20-3EpoxyqueuosineC17H23N5O8425.39Inquiry
BRP-02127116120-47-9LysidineC15H25N5O6.HCl407.85Inquiry
BRP-0212816265-37-5N6-AcetyladenosineC12H15N5O5309.28Inquiry
BRP-0212920299-15-45-Methoxycarbonylmethyl-2-thiouridineC12H16N2O7S332.33Inquiry
BRP-021302140-64-93-MethylcytidineC10H15N3O5257.24Inquiry
BRP-0213129428-50-05-MethoxycarbonylmethyluridineC12H16N2O8316.26Inquiry
BRP-0213229902-55-4N6-GlycinylcarbamoyladenosineC13H16N6O7368.31Inquiry
BRP-0213352745-94-53-(3-Amino-3-carboxypropyl)uridineC13H19N3O8345.31Inquiry
BRP-0213452777-29-43-(3-Amino-3-carboxypropyl)-1-methylpseudoUridineC14H21N3O8359.34Inquiry
BRP-0213555196-46-8WybutosineC21H28N6O9508.49Inquiry
BRP-0213660197-31-15-Methoxycarbonylmethyl-2'-O-methyluridineC13H18N2O8330.29Inquiry
BRP-02137945684-11-7HydroxywybutosineC21H28N6O10524.49Inquiry
BRP-02138872710-82-2N2,7,2'-O-TrimethylguanosineC13H19N5O5325.32Inquiry
BRP-0213989665-83-8(S)-5-(Carboxyhydroxymethyl)uridine methyl esterC12H16N2O9332.27Inquiry
BRP-0214089708-80-5(S)-5-(Carboxyhydroxymethyl)uridineC11H14N2O9318.24Inquiry
BRP-0214191101-00-72'-O-Methyl-N1-methyladenosineC12H17N5O4295.29Inquiry
BRP-021421967829-21-52'-O-Methyluridine 5-oxyacetic acid methyl esterC13H18N2O9346.29Inquiry
BRP-021431353039-95-83-(3-Amino-3-carboxypropyl)-5,6-dihydrouridineC13H21N3O8347.32Inquiry
BRP-021443-(3-Amino-3-carboxypropyl)pseudouridineC13H19N3O8345.31Inquiry
BRP-021452450329-71-05-(Carboxyhydroxymethyl)-2'-O-methyluridine methyl esterC13H18N2O9346.29Inquiry
BRP-021465-Methoxycarbonylmethyl-2-thio-2'-O-methyluridineC13H18N2O7S346.36Inquiry
BRP-02147898543-14-17-AminocarboxypropylwyosineC18H24N6O7436.42Inquiry
BRP-021481415901-62-0Cyclic N6-threonylcarbamoyladenosineC15H18N6O7394.34Inquiry
BRP-0214915763-06-11-MethyladenosineC11H15N5O4281.27Inquiry
* Only for research. Not suitable for any diagnostic or therapeutic use.
Verification code
Inquiry Basket