BOC RNA provides linker phosphoramidites used for DNA/RNA synthesis. Use an amino linker to conjugate biotin, fluorescein, or other modifiers and a reporter group to the 5'end of the oligonucleotide or adheres to the surface.
As the DNA-based microarrays and multi-label diagnostic systems become increasingly important, the demand for easy preparation of functionalized oligonucleotides is also growing. At present, the most common modification is the primary amine at the 5'end of the oligonucleotide. TFA-protected and MMT-protected 5'-amino modifiers can be used in automatic synthesizers or manual coupling to connect various moieties to 5'-primary amines, including fluorescent dyes, biotin, and EDTA.
The acid-labile MMT protecting group is stable to basic cleavage and deprotection conditions and can be used as a "handle" for analysis in RP high-performance liquid chromatography to easily separate oligonucleotides from failed sequences. The MMT group is then removed using acidic conditions.
The MMT linker is the best choice when purification by reversed-phase technique is required.
In the cleavage and deprotection steps, the alkali-labile TFA-group can be easily removed with concentrated ammonia. In the case of directly separating amino-modified oligonucleotides from cleaved and deprotected oligonucleotides, base-labile TFA moieties are used.
Custom manufacturing services are available to help develop or expand phosphoramidite compounds.
If you have any questions or requirements, please feel free to contact us.
Catalog | CAS Number | Product Name | Molecular Formula | Molecular Weight | Price |
BRP-02206 | 139747-14-1 | TFA-Pentylaminolinker Phosphoramidite | C16H29F3N3O3P | 399.39 | Inquiry |
BRP-02207 | 168329-40-6 | PC Linker Phosphoramidite | C39H46N3O7P | 699.78 | Inquiry |
BRP-02208 | 922522-12-1 | ssH-Linker | C38H53N4O5P | 676.83 | Inquiry |
BRP-02209 | 133975-85-6 | TFA-Hexylaminolinker Phosphoramidite | C17H31F3N3O3P | 413.42 | Inquiry |
BRP-02210 | 114616-27-2 | 5'-Amino Modifier | C35H48N3O3P | 589.75 | Inquiry |