Cyanine Labeling of Oligonucleotides

Cyanine labeled oligonucleotides can be used in a variety of experimental techniques such as in situ hybridization, fluorescent in situ PCR, fluorescent probes, and fluorescent in situ sequencing for detection, quantification, and imaging of DNA or RNA. BOC Sciences provides Cyanine labeling of oligonucleotides services to our customers, as well as characterization and validation services for labeled oligonucleotides.

Fig 1. Preparation of Cy3-and Cy5-bis-labeled oligoribonucleotides. (Hagen et al., 2019)

What is Cyanine Labeling?

Cyanine labeling refers to the binding of a Cyanine dye to a target molecule, such as an oligonucleotide, protein, or other biomolecule, for visualization or detection of the target molecule. In a chemical reaction, reactive groups in the Cyanine dye react with specific functional groups on the target molecule to form a covalent linkage. Commonly used functional groups include amine groups, hydroxyl groups, and sulfhydryl groups. In bioconjugation methods, a binding agent with specific affinity is often used to bind to the Cyanine dye to achieve selective labeling of the target molecule. The advantages of Cyanine labeling, including high fluorescence brightness, good photostability, and the ability of multicolor labeling, have made it one of the most commonly used tools in the field of biomarkers.

Cyanine Labeling Oligonucleotide Services

BOC Sciences provides professional Cyanine-labeled oligonucleotide services. Cyanine labeling includes Cyanine3, Cyanine5, Cyanine5.5, Cyanine7 and so on. With Cyanine labeling, fluorescent markers can be introduced into target molecules for their visualization, localization and detection.

• Cyanine3 labeled oligonucleotide services

BOC Sciences can provide Cyanine3-labeled oligonucleotide services. In particular, the chemical structure of Cyanine3 dye consists of an indole ring and two linked pyran rings, making it suitable for fluorescent dye labeling and probe design. Conjugation of Cyanine3 in oligonucleotides can be used for live cell imaging, in vivo imaging and molecular probe studies.

• Cyanine5 labeled oligonucleotide services

Similar steps to Cyanine3 labeled oligonucleotide molecules. When we perform Cyanine5 labeling of oligonucleotides, we take into account that Cyanine5 emits red fluorescence and its maximum emission wavelength is usually around 650 nm. Its range of applications that can be applied after being custom synthesized differs from that of Cyanine3, and is better accomplished to complement Cyanine-labeled oligonucleotides.

• Cyanine5.5 labeled oligonucleotide services

BOC Sciences has rich experience in Cyanine labeling and can provide customers with Cyanine5.5 labeled oligonucleotide services. Cyanine5.5 (Cy5.5) is a near-infrared (NIR) fluorescent dye that emits fluorescence at NIR wavelengths with good light penetration and a low background signal, which makes it suitable for NIR imaging and detection applications. Usually, before we conduct experiments on Cyanine5.5 labeled oligonucleotides, we will analyze in detail the characteristics of Cyanine5.5, its function, and whether it can achieve the purpose after combining with oligonucleotides. After completing the product customization, we will verify the Cyanine5.5 labeled oligonucleotide to ensure that the resulting product has excellent performance.

• Cyanine7 labeled oligonucleotide services

Compared to other types of Cyanine molecules, Cyanine7 is more stable and can maintain a more stable fluorescence signal under prolonged excitation. BOC Sciences' researchers develop Cyanine7-labeled oligonucleotides by selecting the appropriate oligonucleotides for coupling according to their properties, which enables the oligonucleotides to be used in in vivo imaging, tissue sectioning, and analysis.

In addition to the above Cyanine molecules, we also provide the existence of other variants and derivatives, such as Cyanine2 (Cy2), Cyanine5.5 NHS ester, Cyanine7 NHS ester, etc., which have different chemical structures and fluorescence properties, and can be selected and used according to the experimental needs. If you are interested in our services, please feel free to contact us .

Reference

1. Hagen T, et al. Site-specific fluorophore labeling of guanosines in RNA G-quadruplexes[J]. ACS omega, 2019, 4(5): 8472-8479.

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Cyanine Labeling of Oligonucleotides

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