tRNA Sequencing

Translation of the genetic code is crucial to all living organisms. The ribosomes depend on transfer RNA (tRNA) to correctly decipher the genetic information contained within messenger RNA (mRNA). The key to deciphering the genetic code is tRNA which disseminates nucleic acid triplets into correct amino acid identity. BOC Sciences provides the most advanced tRNA sequencing service in the industry which integrate accurate removal of tRNA modification and small RNA sequencing optimization. Our experts are able to efficiently and accurately remove internal and terminal modifications of tRNAs, and thus offer quantitative tRNA profiling of tRNA expression and modification.

Our tRNA Sequencing Services

Our sequencing services are all developed based on comprehensive tRNA transcriptomes reference from all authoritative databases, and we can provide detection of tRNA mutations/variants/isomers and complete data analysis.

• Assessment of tRNA Modification, Aminoacylation and Fragmentation

Our high-throughput tRNA-seq allows for the analysis of tRNAome-wide networks of modifications, aminoacylation, and fragmentation in the same sequencing library to investigate the existence of correlations among modification-modification, modification-aminoacylation, and modification-fragmentation.

• Assessment of tRNA Transcriptome

BOC Sciences' quantitative mature tRNA-sequencing protocol is a fast and relatively simple method to robustly monitor tRNA levels and can be used to explore potential the modifications. Our tRNA-sequencing service is highly reproducible and can represent tRNA levels across a wide range of expression levels, thereby providing reliable data on the tRNA transcriptome.

• Mapping and Quantification of RNA Modifications

There are various chemical modifications of the nucleosides that make up transfer RNAs. However, the structure, location, and degree of modification are difficult to map systematically. With knowledge of tRNA biology, BOC Sciences provides standardized high-throughput tRNA sequencing services to rapidly predict modification sites and monitor tRNA modifications. Our experts can rapidly predict the modification sites by using the reverse transcription-derived signatures in high-throughput transfer RNA-sequencing data and identify tRNA modifications by NGS-based technologies and RNA mass spectrometry.

• Bioinformatics Analysis

BOC Sciences' tRNA sequencing services include rich bioinformatics analysis that can help our clients gain insight into tRNAs in biology and diseases. We provide comprehensive tRNA annotation and bioinformatics analysis, and our bioinformatics analyses services include:

1. Differential expression profiling.
2. Plots for differential expression analysis.
3. Expression levels of different tRNA types grouped by anticodon.

Fig 1. Schematic illustrations of quantitative profiling of tRNA abundance and modification with the application of tRNA-seq. (Behrens A, 2021)

tRNA Sequencing Workflow

• Sample preparation: Ribosomal RNA depletion and fragmentation.
• Efficient full-length cDNA library construction.
• tRNA sequencing analysis: Indexing and alignment, read deconvolution and post-alignment analysis and visualisation.

BOC Sciences' Benefits

• Continuously optimized tRNA sequencing tools and workflows.
• Rich project experience in sequencing data processing.
• Expertise in the field of tRNA bioinformatics.
• Final data delivered to the client is visualized and of publication quality.
• Commercial services for tRNA research in tRNA function and diseases.
• Seamless integration with BOC Sciences' broad portfolio of tRNA services.
• Quality assurance support provided by BOC Sciences' QC department.

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tRNA Sequencing

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