Tocopherol Labeling of Oligonucleotides

BOC Sciences, as a specialized supplier of nucleic acid products, can provide you with a wide range of modified oligonucleotides. Our technical staff with a wide range of expertise and experience in product labeling can facilitate the development of your project.

What is Tocopherol?

Tocopherol is a class of chemically similar natural compounds including α-tocopherol, β-tocopherol, γ-tocopherol and δ-tocopherol. Tocopherol is a class of fat-soluble compounds with antioxidant and vitamin E activity. It plays an important role in cytoprotection, oxidative defense and maintenance of human health.

Fig.1 Different types of tocopherol molecules.

Advantages of Tocopherol Labeling of Oligonucleotides

• After tocopherol labeling in oligonucleotides, tocopherol-affinity interactions can be utilized to bind the oligonucleotide to other biomolecules (e.g., proteins), and then the target molecule can be purified using affinity resins.
• Upon completion of tocopherol labeling of the oligonucleotide, the affinity-tocopherol interaction can be used to bind specifically to the oligonucleotide, and then markers (e.g., fluorescent dyes or enzymes) can be used to detect or visualize the presence and location of the oligonucleotide.
• By introducing tocopherol-modified oligonucleotides as primers, target sequences can be specifically labeled during an amplification reaction or sequencing process so that they can be recognized and analyzed by subsequent detection or sequencing methods.

Services for Tocopherol-Labeled Oligonucleotides

BOC Sciences offers a wide range of oligonucleotide modification services. In addition to cholesterol and fluorescent labeling, we can also offer tocopherol labeling. Based on our advanced technology and rich experience, we are able to provide custom synthesis, process optimization, and scale-up production of tocopherol-labeled oligonucleotides to help you with your project development.

• Custom synthesis of tocopherol-labeled oligonucleotides

• Oligonucleotide sequence design

First, the required oligonucleotide sequence is designed based on the customer's research objectives and application needs. At the same time, it is necessary to determine the position of the marker and introduce into the sequence a modified nucleotide or linker suitable for tocopherol labeling.

• Synthesis of modified nucleotides

The modified nucleotide or linker with the desired function for tocopherol labeling is then synthesized.

• Oligonucleotide synthesis

Subsequently, tocopherol-labeled oligonucleotides are synthesized by ligating the modified nucleotide to other nucleotide units using our advanced oligonucleotide synthesis method.

• Purification and characterization of tocopherol-labeled oligonucleotides

Finally, the synthesized tocopherol-tagged oligonucleotides were purified using purification techniques such as high-performance liquid chromatography (HPLC) or polyacrylamide gel electrophoresis (PAGE). Also, the purified tocopherol-labeled oligonucleotides are characterized using techniques such as UV spectroscopy or capillary electrophoresis to determine their concentration, purity and integrity.

• Process optimization of tocopherol-labeled oligonucleotides

The preparation process needs to be optimized in order to ensure that the product is uniform, reproducible, and can be produced on a large scale. We need to consider systematic experimental design and parameter optimization when performing process optimization to achieve the best labeling and application results. These conditions that can be optimized are the selection of suitable tocopherol labeling sites, the optimization of labeling reaction conditions, the selection of suitable protecting groups and activators, and so on.

• Scale-up production

We ensure that the process for tocopherol labeled oligonucleotides has been validated and optimized before proceeding to scale-up production. After completing this step, we need to select the appropriate reaction scale according to the customer's needs, establish a strict quality control system, and conduct small-scale pilot production and validation batches before finally refining the quality management system to ensure the quality and consistency of the product.

BOC Sciences provides a quality control system to ensure that the labeling of oligonucleotides is reliable. If you are interested in our services, please feel free to contact us.

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Tocopherol Labeling of Oligonucleotides

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