Phosphorylation refers to the biochemical process of adding phosphate molecules to certain organic compounds. BOC RNA has extensive experience in the chemical synthesis of oligonucleotides, with exclusive equipment, reagents and expertise to ensure the high quality of each oligonucleotide. We specialize in providing a wide range of DNA/RNA modification services, including phosphorylation.
As one of the important biochemical reactions, phosphorylation, simply put, is the addition of phosphate molecules/phosphate groups to organic compounds, making them useful for many functions of organisms.
Phosphorylation reactions are particularly important in biology because several biological processes rely on such reactions as apoptosis, inflammation, metabolic regulation, subcellular transport and proliferation.
Phosphorylation of mammals occurs on the side chains of three amino acids:
5' phosphorylation is added via B-cyanoethyl chemistry to the sugar ring at the 5' end of the primer, not the last base. It can be used for splicing, cloning and gene construction as well as ligase-catalyzed ligation reactions. It is also used to block DNA polymerase-catalyzed DNA strand extension reactions in experiments related to exonuclease resistant digestion.
Fig. 1 Phosphorylation and dephosphorylation (Chaudhari M, 2021)
Phosphorylation is a reversible reaction. Most enzymes and receptors are activated and inactivated by phosphorylation and dephosphorylation (as opposed to the phosphorylation process), respectively. Phosphorylation of enzymes acts as an on/off switch, altering specific activity or function.
The phosphate molecule itself is a very important element in biomolecules. Researchers have found that phosphorylation of a large number of metabolites (intermediates or end products of metabolism) occurs, leading to the formation of the corresponding phosphates in cells.
Several molecules can undergo phosphorylation and dephosphorylation. The three most common forms of phosphorylation are:
| Phosphorylation | Short Code | Price |
| 5'-Phosphate | 5'-P | Inquiry |
The expert team at BOC RNA has extensive experience in the synthesis and modification of custom oligonucleotides. We are committed to providing end-to-end customized DNA/RNA modification services, from consulting, DNA/RNA oligonucleotide design, production and purification, to quality control and delivery. Please contact us for more details.
Reference
Phosphorylation is the process of adding phosphate groups to organic compounds, playing a key role in regulating biological functions such as enzyme activity, protein interactions, and energy metabolism. It is crucial for processes like apoptosis, inflammation, and cell transport.
Phosphorylation acts as a molecular switch, activating or deactivating enzymes by adding a phosphate group. This reversible process is essential for regulating enzyme activity and cellular functions, influencing pathways such as metabolism and signaling.
Phosphorylation can occur on proteins, nucleic acids, and other biomolecules. The most common forms include protein phosphorylation (e.g., tyrosine, serine, threonine), glucose phosphorylation, and DNA phosphorylation, each playing a vital role in cellular processes.
5'-Phosphorylation is commonly used in cloning, gene construction, and splicing applications. By adding a phosphate group to the 5' end of primers, it enables ligase-catalyzed reactions and prevents DNA polymerase extension, which is useful in experiments involving exonuclease resistance.
Phosphorylation of nucleotides, especially at the 5' end, is essential for initiating RNA and DNA synthesis. It plays a key role in the formation of RNA primers and DNA strands, aiding in processes like replication, transcription, and amplification.
Yes, we offer flexible phosphorylation modification services with various specifications, including custom concentrations and scales. Our team can work with you to tailor the modification to meet the specific requirements of your research project, whether for small-scale or large-scale production.
