Description: 3'-amino-Modifier CPG contains an amino group and is protected by the base-vulnerable Fmoc group, designed to functionalize the 3'-terminal of the target oligonucleotide by introducing a primary amine.
Description: MGB Eclipse® CPG is a cutting-edge biomedical breakthrough designed for utilization in diverse disease research, encapsulating a distinctive form of DNA alteration, denoted as MGB (Minor Groove Binder) Eclipse® CPG. This unparalleled composition manifests remarkable avidity when binding to precise DNA sequences.
Description: 3'-CPR II CPG, a vital tool in molecular biology, is renowned for unraveling the elusive role of Toll-like receptor 9 (TLR9) in shaping the immune system. In the domain of immunotherapy, the adjuvant supplements DNA vaccines and jumpstarts immune responses to eradicate cancer and infections. Dive into the intriguing world of 3'-CPR II CPG as its therapeutic potential has undergone rigorous scrutiny in pre-clinical and clinical trials.
Synonyms: 3'-CPR II CPG 1000; 3-(4,4'-Dimethoxytrityloxy)-2,2-(dicarboxymethylamido)propyl-1-O-succinoyl-long chain alkylamino-CPG; 3'-CPR II CPG 1000Å
3'-PT-Amino-Modifier C6 CPG
Description: 3'-PT-Amino-Modifier C6 CPG, a specialized product that plays an essential role in the biomedical industry, facilitates the efficient and specific synthesis of oligonucleotides by serving as a solid support throughout the synthesis procedure. Its exceptional utility in the development of antisense drugs targeting an array of diseases, such as cancer, viral infections, and genetic disorders, underscores its criticality in biomedicine. With its high yield and purity, this product empowers researchers to create custom oligonucleotides, thereby rendering it an indispensable tool in the field of biomedicine.
Description: 3'-Cholesteryl-TEG CPG is a bioactive and indispensable chemical used in biomedical research for drug discovery and development. Renowned for its exceptional capacity in oligonucleotide synthesis, where cholesterol conjugation is absolutely indispensable, it holds great promise in the fight against cholesterol-dependent diseases such as atherosclerosis and hypercholesterolemia. Its prowess in the arena is unchallenged and highly sought after.
Description: 3'-Spacer C3 CPG can act as a blocker of exonuclease and polymerase activity at the 3' terminal. dSpacer is used to introduce stable racemic sites in oligonucleotides. PC Spacer is a photocracking C3 spacer modifier. Spacer phosphamides C3, C9, C12, and C18 are used to insert spacer arms in oligonucleotides, and these compounds can be added multiple times when longer spacer arms are needed.
Description: AquaPhluor® 593 CPG is a fluorescent dye used to analyze RNA and DNA sequences in the biomedical industry. It is often used in conjunction with oligonucleotides to detect and quantify the expression levels of genes associated with diseases such as cancer or viral infections. Its unique water-soluble nature allows for easy use in aqueous environments.
Description: Cyanine 3 CPG, a premium fluorophore widely employed in biomedical research, serves as an indispensable tool for the precise detection of nucleic acids. Specifically, it presents a remarkable ability to label and detect targeted DNA and RNA sequences, guaranteeing higher sensitivity in analyses like microarray analysis and sequencing. Furthermore, its excellent fluorescent properties allow for the traceability of these sequences with great accuracy.
Description: Cyanine 5 CPG is a fluorescent labeling reagent used for DNA synthesis, DNA fragment detection, sequencing and genotyping. The product is commonly used in biomedicine to visualize and identify DNA in microarrays, hybridization analysis, and gene expression analysis. Through its sophisticated design, CPG enables an unprecedented level of precision in DNA analysis.
Description: 3'-BHQ-1 CPG is a high-quality and specialized phosphoramidite that is crucial in the synthesis of oligonucleotides used in biomedical research for applications such as DNA sequencing, gene expression analysis, and antiviral drug development. Its unique black-hole-quencher (BHQ) design allows for the detection of specific genetic mutations and gene expression patterns, making it an essential tool in the study and treatment of diseases such as cancer and viral infections.
Description: 3'-BHQ-2 CPG is a strategically-crafted synthetic phosphorothioate oligonucleotide, masterfully adorned with a 3'-Black Hole Quencher (BHQ-2) and 5'-dimethoxytrityl protected group. This illustrious product is pivotal in the oligonucleotide synthesis movement which looks towards the development of revolutionary oligonucleotide-based therapeutics to regulate gene expression, with exceeding ability. The medical industry envisages it to be the solution in the fight against malignant diseases, such as cancer and viral infections that pose a significant threat to human existence.
Description: 3'-BHQ-3 CPG, a modified nucleotide, is a vital component of oligonucleotide synthesis for scientific research. The merging of black hole quencher (BHQ) and 3' cytosine (C) modification endows it with magnified quenching capacity for fluorescent probes and enhanced nuclease stability that is imperative in the domain of diagnostics and therapeutics for addressing viral, cancerous, and immune disorders.
Description: 3'-Thiol-Modifier S-S CPG is used to introduce a 3'-Thiol junction with 3 and 6 atomic intervals in oligonucleotides. 3'-Dithiol Serinol CPG is used to introduce a Dithiol group at the 3' -terminus, and in combination with Dithiol Serinol Phosphoramidite, oligonucleotides with multiple thiol groups at the 3' terminus can be simply produced, which is well suited for conjugation to gold surfaces.
Description: 3'-Uaq Cap CPG is an exquisite biomolecular entity found within the biomedical realm, unveiling its prominence as a revolutionary instrument. This specialized compound predominantly caters to the research and development of nucleotide sequences and oligonucleotides, infiltrating the dynamic spheres of drug development and genetic explorations encompassing ailments of formidable nature, including cancer, viral infections is and intricate genetic mutations.
Synonyms: 5'-Dimethoxytrityl-Uridine, 2'-(anthraquinone-2-yl-carboxamido)-3'-succinoyl-long chain alkylamino-CPG; 3'-Uaq Cap CPG 1000Å
CDPI3 MGB™ CPG
Description: CDPI3 MGB™ CPG is an exquisitely tailored and purposeful compound that garners marked proficiency within the realm of biomedicine, and has demonstrated efficacy in addressing diverse manifestations of cancer. Its unique mechanism of action bespeaks its ability to regulate crucial enzymes involved in tumor expansion, exclaiming its aptitude for both solid tumors and leukemia. Moreover, corresponding research has unveiled the possibility of applying this compound as an efficacious treatment modality for autoimmune disorders.
Description: 3'-Phosphate CPG, a widely employed constituent in the realm of biomedicine, serves as a crucial building block in the creation of oligonucleotides and a plethora of therapeutic agents, namely antiviral and anticancer drugs, as well as gene therapy. With its capacity to stimulate RNA and DNA amplification, this product occupies a prominent place in genetic disease research, illustrating its indispensability and utility.
Description: 3'-Glyceryl CPG is a biomolecular tool that has revolutionized the biomedical industry, enabling the synthesis and modification of oligonucleotides with unrivaled efficiency and precision. Its application in antisense therapy, where targeted mRNA sequences are identified and treated with specific oligonucleotides, has led to groundbreaking advancements in the realm of cancer and genetic disorder treatment. The expansive potential of this tool is limitless, holding promise for the development of novel therapeutics that could remedy even the most complex viral infections.
Description: 3'-Thiol-Modifier C3 S-S CPG is an indispensable entity within the biomedical sector assuming a pivotal function delineating the alteration of DNA. Its significance is paramount, particularly in the realm of nucleic acid research and development, with a specific focus on oligonucleotide construction. The utilization of this prodigious entity holds immense value in the field of drug discovery, where targeted therapeutics endeavor to combat multifarious maladies by honing in on precise genes and genetic sequences.
Description: 3'-Thiol-Modifier 6 S-S CPG, a chemical compound widely utilized in DNA synthesis and modification within the biomedical industry, functions as a support solid phase for the production of thiolated DNA molecules. These molecules have potential applications in diagnostics and therapeutics for diseases which include cancer and genetic disorders.
Description: DesthiobiotinTEG-CPG, a popular chemical compound, is frequently employed to extract biotin-binding proteins. Its application has extended to biomedicine, where it is employed to investigate and grasp the intricate biological pathways concerning biotin and its binding proteins. Employing DesthiobiotinTEG-CPG has propelled the progress of developing new therapeutic drugs for targeted diseases.
Controlled Pore Glass (CPG) is a critical solid support used in solid-phase oligonucleotide synthesis, directly influencing coupling efficiency, yield, and batch-to-batch consistency in DNA and RNA manufacturing. In both research and CDMO production environments, the selection of appropriate CPGs—including pore size, loading capacity, and surface chemistry—is essential for achieving reliable and scalable oligonucleotide synthesis. This page provides a practical overview of CPGs for oligo synthesis, covering key technical specifications, application scenarios, quality control considerations, and supply factors relevant to process development, scale-up, and long-term manufacturing programs.
What Are CPGs for Oligo Synthesis?
Controlled Pore Glass (CPG) is a rigid, inorganic solid support widely used in solid-phase oligonucleotide synthesis, particularly in phosphoramidite-based oligonucleotide synthesis. During DNA and RNA synthesis, CPG serves as the stationary phase onto which the first nucleosides used in oligonucleotide synthesis are immobilized, enabling stepwise chain elongation with high efficiency and reproducibility.
In CDMO and manufacturing environments, the physical and chemical properties of CPG play a critical role in determining coupling efficiency, process robustness, and batch-to-batch consistency. An appropriate CPG must provide:
Uniform and accessible pore structure to support long-chain growth
Chemically stable surface functionalization compatible with DNA and RNA phosphoramidite chemistry
Mechanical strength suitable for automated and repeated synthesis cycles
As oligonucleotide chains grow longer, diffusion limitations and steric hindrance become increasingly significant. Therefore, pore size and surface loading of CPG are key risk factors in long and complex oligonucleotide manufacturing projects, especially for RNA, modified RNA, and therapeutic oligos. Our CPGs are engineered with these challenges in mind, supporting process development, scale-up, and routine manufacturing for DNA and RNA oligonucleotides.
Examples of CPGs
1TAMRA CPG
Because tetramethylrhodamine (TAMRA) is not base-stable, procedures for cleaving and deprotecting labeled oligonucleotides must be carefully considered. For example, to simplify the preparation of TAMRA oligonucleotides, 3'-TAMRA CPG for 3' labeling and TAMRA-dT for in-sequence labeling are available.
2Fluorescein CPG
Fluorescein CPG is traditionally used to add fluorescein labeling to the 3'-end. For example, 3'-(6-FAM) CPG effectively blocks polymerase extension and exonuclease digestion at the 3'-end.
3DMT-Phosphate-CPG
The phosphate functional group on the surface of DMT-Phosphate-CPG usually carries a DMT protecting group. DMT is a commonly used protecting group that protects the phosphate group from non-specific reactions during nucleotide synthesis. The DMT protecting group can be easily removed at the end of the synthesis to expose the phosphate group for further modification. CPGs have a pore size and pore structure that can be tuned similar to that of conventional CPGs. This controllable pore size facilitates efficient solid-phase synthesis of oligonucleotides and ensures good attachment and stability of the phosphate functional groups.
4Aminopropyl-CPG
Aminopropyl-CPG is a CPG modified by an aminopropyl functional group, and we can provide products with pore sizes ranging from 300 Å-2000 Å. The products can be used for the amination modification of nucleotides, and by introducing an amino functional group, the chemistry and reactivity of nucleotides can be extended.
Features of CPGs
Adjustable Pore Size and Pore Structure
The pore size and pore structure of CPGs can be tuned as needed, typically in the range of 3-30 nanometers. This controllable pore size and pore structure helps ensure efficient solid phase synthesis of oligonucleotides.
Surface-active
CPGs have a large amount of surface activity, providing enough binding sites to allow nucleotide immobilization and chemical reactions to take place during the synthesis process.
Chemical Stability
CPGs have good chemical stability and can be used under common nucleotide synthesis reaction conditions. They can tolerate synthesis conditions such as alkaline conditions, organic solvents, and high temperatures, thus ensuring high-quality oligonucleotide synthesis.
Tunable Synthesis Scale
CPGs can be scaled and adjusted as needed for both small-scale research and large-scale industrial production.
Key Specifications of Our CPGs
1Optimized Pore Size Distribution for Process Reliability
We offer CPGs with controlled and reproducible pore sizes to support different synthesis strategies and oligo lengths:
500 Å – suitable for short DNA oligonucleotides
1000 Å – optimized for medium-length DNA and RNA oligos
2000 Å – recommended for long oligonucleotides, RNA, and modified sequences
Larger pore sizes improve reagent diffusion and reduce steric hindrance, helping maintain coupling efficiency during extended synthesis cycles common in CDMO manufacturing workflows.
2High and Controlled Loading Capacity
Our CPGs for oligo synthesis provide high and precisely controlled loading capacity, supporting predictable synthesis scale and consistent performance in CDMO manufacturing.
Optimized loading levels are selected to balance yield and coupling efficiency, especially for long and modified oligonucleotides.
Tight batch-to-batch control ensures reproducible loading capacity across production runs, minimizing process variability.
Custom loading options are available to support specific synthesis workflows, scale-up strategies, and long-term manufacturing programs.
3Narrow Particle Size Distribution
Carefully controlled particle size distribution ensures:
Stable reagent flow
Minimal back pressure in automated synthesizers
Consistent performance across different instruments and synthesis scales
This contributes to instrument-to-instrument reproducibility and reduced process variability.
4Chemically Stable Surface Functionalization
The CPG surface is functionalized to provide:
Strong and reliable linker attachment
Compatibility with high-purity phosphoramidites
Resistance to repeated synthesis, deprotection, and washing cycles
This stability helps reduce the risk of premature cleavage or surface degradation during long manufacturing campaigns.
Applications of CPGs for Oligo Synthesis
Our CPGs are designed to support process development and routine manufacturing of oligonucleotides in CDMO and industrial settings.
DNA Oligonucleotide Manufacturing
Widely used in the process development and routine production of DNA oligonucleotides, our CPGs support:
Diagnostic primers and probes
Therapeutic and research-grade DNA sequences
Programs requiring consistent performance across multiple batches
RNA and Modified RNA Synthesis
RNA and modified RNA synthesis place higher demands on solid supports due to increased steric hindrance and sensitivity to reaction conditions. Our 1000 Å and 2000 Å CPGs are optimized for:
RNA oligonucleotides
siRNA and antisense oligos
Chemically modified RNA produced from modified nucleosides and RNA building blocks
Enhanced pore accessibility helps mitigate common risks associated with long RNA synthesis.
Long and Complex Oligonucleotide Projects
For long or complex sequences commonly encountered in CDMO manufacturing projects, our CPGs are engineered to:
Reduce diffusion limitations in later synthesis cycles
Maintain coupling efficiency when using phosphoramidites for long oligonucleotides
Support higher final purity and yield
Compatibility with Automated Synthesizers
Our CPGs are designed for seamless compatibility with automated DNA and RNA oligonucleotide synthesizers, supporting stable and reproducible performance in both development and manufacturing environments.
Controlled particle size distribution ensures uniform reagent flow and minimizes back pressure during automated synthesis cycles.
Mechanical stability supports repeated synthesis, washing, and deprotection steps without particle degradation.
Broad instrument compatibility enables consistent performance across commonly used research and production-scale synthesizer platforms.
Quality Control and Batch Consistency
Our quality control system is designed to support process qualification, scale-up, and long-term manufacturing consistency, where material variability can directly impact downstream validation and regulatory timelines.
Surface Loading Verification
Each batch is tested to confirm:
Target loading capacity (μmol/g)
Uniform surface functionalization
This ensures predictable synthesis scale and reproducible oligo yields across batches.
Pore Size and Particle Distribution Analysis
Routine testing verifies:
Pore size distribution
Particle size uniformity
These parameters are critical for reagent accessibility, flow stability, and instrument-to-instrument consistency.
Moisture Content Control
Moisture control is essential for minimizing risk in phosphoramidite-based synthesis. Our CPGs are processed and packaged to maintain low moisture levels, helping protect coupling efficiency and reagent stability.
Documentation and Traceability
Each batch is supplied with:
Certificate of Analysis (COA)
Batch identification and full traceability
This documentation supports process transfer, scale-up validation, and customer or regulatory audits in CDMO environments.
Why Choose Us as Your CPG Partner
Manufacturing-Focused Design: Our CPGs are engineered with a deep understanding of solid-phase oligonucleotide synthesis, supporting reliable performance in process development, scale-up, and routine CDMO manufacturing.
Consistent Quality & Controlled Change: We maintain strict batch-to-batch control and structured change management to minimize variability and reduce risk in long-term manufacturing programs.
Customization to Fit Your Process: We offer flexible options for pore size, loading capacity, and surface chemistry to align with your specific synthesis workflows and project requirements.
Reliable Supply & Technical Support: Our scalable manufacturing capabilities and responsive technical team support both initial supplier qualification and long-term material supply.
Ready to Discuss Your CPG Requirements?
Selecting the right CPG is critical for achieving consistent performance in solid-phase oligonucleotide synthesis, especially in CDMO and manufacturing environments where scale, reproducibility, and supply reliability matter. Our technical and sourcing teams can support CPG selection, specification alignment, and customization based on your synthesis platform, oligo length, and project stage. Please contact us to discuss your requirements or submit an RFQ to evaluate supply options for your program.
Frequently Asked Questions (FAQ)
What pore size CPG is recommended for long oligonucleotide synthesis?
For long DNA or RNA sequences, 1000 Å or 2000 Å CPG is generally recommended to improve reagent diffusion and maintain coupling efficiency during extended synthesis cycles.
How does loading capacity affect CDMO manufacturing projects?
Loading capacity influences both yield and coupling efficiency. Controlled and reproducible loading is essential for minimizing batch-to-batch variability in long-term manufacturing programs.
Do you provide COA and batch traceability?
Each batch is supplied with a Certificate of Analysis (COA) and full traceability to support audits and quality reviews.
Can you support material supply during process scale-up?
Yes. We support material supply from early process development through scale-up and routine manufacturing.
How should CPGs be stored?
CPGs should be stored in a dry, tightly sealed container at room temperature to maintain surface functionality and consistent performance.
* Only for research. Not suitable for any diagnostic or therapeutic use.
