DNA/RNA Modification

BOC RNA can provide different forms, different lengths, and large quantities of chemically modified DNA/RNA. Our custom synthesis service can be flexible and diverse to fit any specifications.

Chemical Modification

In the classic central dogma, genetic information usually flows from DNA to RNA and is then expressed as proteins. However, in addition to the genetic code, nucleic acids also contain another layer of complex epigenetic modification systems dominated by chemical modifications to expand their information encoding capabilities. These modifications participate in a variety of important biological processes by regulating gene expression networks, also known as epigenetic modifications. Epigenetic modifications usually include chemical modifications to DNA, RNA, and proteins, and we refer to chemical modifications to DNA and RNA as nucleic acid modifications.

Nucleic Acid Modification

Nucleic acid modifications refer to the modification method of introducing additional chemical groups on DNA or RNA bases, which does not alter the arrangement of gene sequences but greatly improves the ability of bases to encode genetic information. More and more studies have shown that various DNA and RNA modifications actively participate in the regulation of various life processes, including embryonic development and organ differentiation. Dysregulation of these modifications has the potential to cause disease and even death in humans.

DNA Modification

Each of the four bases of DNA has a different modification, with the 5-methylcytosine (5mC) modification being one of the most abundant. As an epigenetic marker, 5mC is closely linked to other chromatin factors and is involved in a wide range of developmental and physiological processes, even in human diseases, with a broad impact on gene expression.

N6 -methyl modification (6mA) is another important DNA modification and was initially thought to be the most prevalent DNA modification in bacteria, however, recent studies have found that it is also present in some eukaryotes at much lower levels than the proportion of modifications found in bacteria.

RNA Modification

Among about 150 kinds of RNA modifications, m6A is the most abundant modification on mRNA and plays an important role in the regulation of gene expression. It is involved in multiple biological processes such as embryonic development, neuron regulation, and tumorigenesis.

Fig. 1 Diverse modifications on nucleic acids and their possible functions (Chen LQ, 2020)

We provide the following modifications:

Our labeling and fluorescent modifications include:

Services process

Service Advantages

• Flexible synthesis scales: 50 nmol, 100 nmol, 200 nmol, 1 µmol, 5 µmol, and 10 µmol. Bulk orders are available upon request
• Provide detailed pre-service guidance and after-service follow-up
• Experienced team: scientists and staff with over 15 years of experience in oligo synthesis and modifications
• Provide multiple purification methods
• High quality: QC analysis by MS, HPLC, or PAGE, and comprehensive quality control reports (HPLC/MS)
• Competitive price
• 24 h delivery

Contact us now to get technical advice and more information. Our technical support team will review and provide a quotation as soon as possible!