RNA synthesis is a key molecular biology process underlying myriad applications from basic research to therapeutic development. BOC Sciences provides you with an elaborate portfolio of RNA synthesis reagents for the accurate and effective creation of various kinds of RNA molecules. Most of these reagents are used in a wide range of methodologies for which the quality is important to obtain reliable results in research as well therapeutic contexts.
RNA synthesis is the process of producing ribonucleic acid (RNA) molecules using a DNA template. Given that RNA is the link between DNA and protein synthesis, this process plays an essential role in gene expression. Different methodologies have developed to synthesize RNA tailored for specific applications, such as in vitro transcription (IVT), chemical synthesis methods or a more novel approach utilizing an in vivo system.
RNA synthesis reagents are essential for generating RNA with high accuracy and yield. In research, these reagents are used to study gene function, regulatory mechanisms, and cellular processes. In therapeutic applications, RNA synthesis is pivotal for developing RNA-based drugs, vaccines , and gene therapies. High-quality reagents ensure the production of RNA with the desired characteristics, such as stability and functionality, which are critical for successful outcomes in these applications.
The cap analog structure itself contains many modifiable sites, such as sugar rings, phospholipid bonds, base substitutions, etc. Combined with the crystal structure of the cap analog-specific binding protein eIF4E, more novel cap analogs with better effects can be designed. The new structure of cap analogs need to consider: mRNA transcription efficiency, capping rate, stability, translation efficiency, immunogenicity and other key factors. BOC Sciences provides a range of cap analogs designed to support the efficient synthesis and functionality of RNA transcripts.
CPG is made of glass (silica). The original glass material is ground, sieved and treated with acid and alkali to form an irregular sphere with many pores inside, and the pores inside the sphere constitute the synthesis site of oligonucleotides. The size of the pore space determines the synthesis length of the oligonucleotide: a large pore space is suitable for the synthesis of long fragments, and vice versa, a small pore space is suitable for the synthesis of short fragments.
Nucleoside is a general term for a class of glycosides. Nucleosides are the building blocks of nucleic acids and nucleotides. Nucleosides are condensed from D-ribose or D-Z-deoxyribose and pyrimidine base or purine base. Nucleosides are generally colorless crystals, insoluble in common organic solvents, soluble in hot water. The nucleoside generated from D-ribose is called ribonucleoside, which is involved in RNA composition, and the nucleoside generated from D-alpha-deoxyribose is called deoxyribonucleoside, which is involved in DNA composition.. BOC Sciences offers a variety of nucleosides, including modified versions, which are used to tailor RNA molecules for specific functions and applications.
Nucleotides are the monomers that make up DNA and RNA and are essential for cell growth, division, and transmission of genetic information. Nucleotides are a very important class of compounds in living organisms, consisting of three substances: purine or pyrimidine bases, ribose or deoxyribose, and phosphoric acid, which are the basic units of nucleic acids. Nucleotides are not only involved in the formation of nucleic acids, but many single nucleotides also have important biological functions. In addition, certain nucleotide analogs can interfere with nucleotide metabolism and can be used as anticancer drugs. BOC Sciences provides high-purity nucleotides, ensuring optimal performance in RNA synthesis and related applications.
Phosphoramidite monomers are the most important starting materials for nucleic acid drugs. In order to increase the resistance of oligonucleotides to nuclease, improve mRNA targeting, improve pharmacokinetic properties, reduce immunogenicity, and prolong the half-life of the drug, among other effects, the modification and modification of phosphoramidite monomers has been ongoing at BOC Sciences.
In vitro transcription instead uses RNA polymerases to synthesize the RNA from a DNA template. It is a prevalent technique to clone DNA in almost any sequence-specific manner so that large quantities of RNA can be produced. High quality nucleotides and cap analogs are used to ensure the RNA produced is high quality for research and therapeutic applications.
There are four major chemical synthetic methods of DNA/RNA:
In which, phosphitetriester method is commonly used for DNA/RNA solid-phase synthesis nowadays. In phosphitetriester method, DNA/RNA chemical synthesis is carried out by a stepwise addition, the synthesis process contains four chemical reactions:
As DNA/RNA contain at least one -OH group, the introduction of the suitable protecting groups enables the oligo to convert to the respective phosphoramidite, and then it can couple the latter. Therefore, phosphoramidite coupling reaction makes phophoramidites the most important building blocks in DNA/RNA synthesis.
In vivo systems involve using living cells to produce RNA. Both bacterial and eukaryotic expression systems are employed, depending on the complexity of the RNA molecule and the desired modifications. Bacterial systems are typically used for simpler RNA constructs, while eukaryotic systems are employed for more complex RNA molecules with post-transcriptional modifications.
Use of Bacterial and Eukaryotic Expression Systems
To achieve efficient RNA production, several factors must be carefully managed:
RNA synthesis reagents from BOC Sciences are utilized in a wide array of applications:
Choosing RNA synthesis reagents from BOC Sciences offers several advantages:
For more information on our RNA synthesis reagents, contact us for more about your needs. The expertise of our scientists, engineers and support team are at your disposal.
BOC Sciences is a leading provider of DNA and RNA products and services that meet the needs of researchers and pharmaceutical companies worldwide. Its broad product portfolio includes various types of oligonucleotides, DNA/RNA synthesis feedstocks, RNA interference tools, and advanced drug delivery systems, among others. Designed to promote cutting-edge research and innovative therapeutic solutions, at BOC Sciences you are sure to find the right product for you.
Global Supplier of High-Quality RNA / DNA Products & Services.
