RNA interference (RNAi) refers to the phenomenon of efficient and specific degradation of homologous mRNA induced by double-stranded RNA (dsRNA), which is highly conserved during evolution. It is an important tool for the study of gene function and is emerging as a tool for gene therapy research in viral diseases, genetic diseases and tumours. At BOC Sciences, our scientists apply advanced biotechnology to develop new RNAi products and RNAi services.
RNA interference (RNAi) technology uses double-stranded RNA to efficiently and specifically degrade intracellular homologous mRNAs to block the expression of target genes, resulting in a phenotype of target gene deletion.
After processing by Dicer (a nuclease with RNAase III activity), the longer double-stranded RNAs (above 30nt) in the cell are first degraded to form small interfering RNAs (siRNAs) of 21-25nt, which efficiently target the target mRNA. Thus, siRNAs are important intermediates for gene silencing and sequence-specific RNA degradation. The shorter double-stranded RNAs cannot be efficiently processed into siRNAs and therefore cannot mediate RNAi. siRNAs have a specific structural feature, namely a 5' end phosphate group and a 3' end hydroxyl group, with two bases protruding from the 3' end of each of its two strands. The antisense strand in siRNA guides the synthesis of a ribosome called RNA-induced silencing complex (RISC), and then RISC-mediated cleaves the region complementary to the siRNA antisense strand in the target mRNA molecule, thereby achieving interference function of target gene expression.
At present, RNA interference methods mainly focus on two aspects: chemically synthesized siRNAs and Vector-based shRNA.
|Stability||High||Low, easily degradable and short-lived|
|Duration of action||Longer duration of action, lasting several weeks or more||Short duration of action, typically around one week|
|Mode of introduction into cells||Plasmid vector, general transfection; viral introduction||Common transfection|
|Expression mode||Polymerase type II and III promoter driven; inducible expression system available; can co-express with reporter genes, visualise cell import rate||Direct chemical synthesis|
|Detection time of inhibition||Dependent on half-life of target protein|
BOC Sciences' RNAi Services team's technical expertise can help clients achieve maximum value from their RNAi technology and avoid a number of experimental errors. We are committed to providing the highest quality RNAi services for scientific research at the lowest price. BOC Sciences provides the following customized RNAi services for your scientific research:
Please contact us for more information. Experimental details will help us provide an accurate quote.