siRNA, as the most used RNAi tool to induce short-term gene silencing in mammals, now is showing a big therapy potential.
Small interfering RNA (siRNA), sometimes called short interfering RNA or silencing RNA, is a type of double-stranded non-coding RNA molecule, usually 20-27 base pairs in length. siRNA can specifically target specific mRNA for degradation and prevent translation, thereby interfering with the expression of specific genes with complementary nucleotide sequences. siRNA can also be used to knock down non-protein-coding genes, such as long non-coding RNA (lncRNA). The use of siRNA can achieve transient silencing in a variety of cell lines, and the experiments are usually limited to a short time range of about 2-4 days.
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Ultrapure siRNA molecules with defined sequences usually synthesized by automated solid-phase synthesis employing 2′-hydroxyl protecting groups. This method can provide highly pure, stable, and modified siRNA. After the duplex siRNA that enters the cell is combined with RISC, it is unwound and the sense strand is cut with an endonuclease. Then, the remaining antisense strand-RISC complex binds to the target mRNA, causing it to be cleaved and degraded, thereby triggering gene silencing.
Figure 1: siRNA mechanism (from Wikipedia).
siRNA is widely used to assess the individual contribution of genes to various cell phenotypes, including cell division, apoptosis, signal transduction, and cell differentiation. siRNA screening has been used to identify new pathways and has a major impact on validating targets for many cellular processes and diseases, including cancer, HIV infection, and hepatitis.
In view of the great potential of siRNA to target any gene for therapeutic intervention, more and more scientists are evaluating the role of siRNA as a therapeutic agent. Currently, FDA has approved two siRNA drugs (Onpattro and Givlaari).
Due to the negative charge, siRNA must be transfected by cationic lipids, polymer-based transfection reagents, electroporation, or chemical modification on the duplex to help cells absorb and transfect.
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siRNA (small interfering RNA) is a short double-stranded RNA that guides the RNA-induced silencing complex (RISC) to degrade target mRNA, enabling precise and transient gene knockdown.
We provide high-purity, sequence-specific siRNA synthesis, including chemical modifications and fluorescent labeling, to meet diverse experimental needs in gene function and pathway studies.
siRNA can be delivered using cationic liposomes, polymer-based transfection, electroporation, viral vectors, or chemically modified duplexes to ensure efficient uptake in different cell types.
Chemical modifications enhance siRNA stability, increase cellular uptake, and allow longer-term gene silencing while reducing degradation and off-target effects, enabling more reliable functional studies.
Yes, siRNA libraries allow systematic knockdown of multiple genes, supporting functional screening, pathway analysis, and target validation across various cell models and experimental conditions.
We offer expert guidance on sequence selection, modification strategies, and delivery methods to optimize knockdown efficiency, reduce off-target effects, and support reproducible experimental results.
