BOC RNA has built up critical expertise in oligo analysis and purification. In the analysis area, we have developed a hexafluoroisopropanol system to analyze the oligo and can get a better result. In the purification area, we have developed RP-IPC to isolate and purify the oligo with high efficiency.
Oligonucleotide analysis is a method for studying the sequence of oligonucleotides in DNA or RNA. It can be used to determine the length, sequence and chemical structure of oligonucleotides and to reveal their role in biological processes. Oligonucleotide analysis includes several different techniques, such as electrophoresis, mass spectrometry and sequencing.
In oligonucleotide analysis, electrophoresis is a common technique used to separate and quantify oligomers of DNA or RNA. Electrophoresis causes DNA oligos or RNA oligos to move gradually according to size and shape and be separated into different bands by applying an electric field to a charged gel or graphene. These bands can be quantified or identified using dyes or chemical reactions.
Mass spectrometry is another technique that is useful in oligonucleotide analysis. By comparing the chemical molecular mass and molecular formula of an oligonucleotide with naturally occurring isotopes, mass spectrometry can determine the mass and sequence of the oligonucleotide. It can also be used to identify and quantify chemical modifications in oligonucleotides.
Sequencing can determine the length, structure and chemical properties of DNA or RNA molecules and provide insight into their function in biological processes. Sequencing technologies include Sanger sequencing, next-generation sequencing and single-molecule sequencing. They all have their own unique applications and advantages and disadvantages.
Each oligo is coupled sequentially to the growing chain via phosphoramidite method during oligo synthesis. In each coupling cycle, a small amount of the oligonucleotide chains will not undergo base extension, resulting in the accumulation of impurities, these impurities can be failure sequences, shorter sequences, longer sequences, chemically-modified sequences, fluorescence-labeled sequences. These impurities may affect the experiment. Therefore, these impurities must be removed after synthesis.
Then how do you know which purification method is a right choice for you? Your choice of purification should be based on the following factors:
|Service Category||Method||Detail||Request a Quote|
|Oligo Analysis Services||Hexafluoroisopropanol system||As the high molecule weight (5000-15000), high electronegativity and hydrophobicity of the oligo, we developed a hexafluoroisopropanol system to analyze the oligo due to weak retention and poor peak sharp using traditional RP-HPLC.||Inquiry|
|Oligo Purification Services||Desalt||Desalting is a basic method. All of our oligos are desalted.||Inquiry|
|Cartridge||Cartridge purification removes failure sequences from your final mixture.||Inquiry|
|RP-HPLC||IE-HPLC (ion-exchange HPLC) is based on the number of charged groups (phosphate) in the oligo backbone. The resolution of this method is very high, but it's not recommended for purifying oligo with more than 40 bases as the purity would reduce.||Inquiry|
|RP-IPC||Due to the low efficiency of IE-HPLC, we developed RP-IPC (reversed-phase ion-pair chromatography) instead to get the oligo isolated and purified with high efficiency.||Inquiry|
|PAGE||PAGE is purified based on size and conformation. The purity using PAGE usually can be 95% or more. However, it can only purify a small amount of oligo at a time and the yield is very low.||Inquiry|
|Gel Filtration||Gel filtration is based on molecular weight. The operation is easy and can remove the salts that have been purified by HPLC.||Inquiry|
|Combination Purification Method||Oligos are best purified by different methods or combinations of methods depending on the application.||Inquiry|
With the combined knowledge of the different business units of our parent company BOC Sciences, we are able to help you succeed in your drug discovery and biomedical research.