mRNA Products

Description: Firefly Luciferase (Firefly luc) expresses a luciferase protein originally extracted from fireflies. The enzyme relies on ATP to catalyze the oxidation of D-fluorescein to produce chemiluminescence at a wavelength of about 560 nm. Firefly luc is a commonly used bioluminescence reporter gene used in gene regulation and function studies. It emits biofluorescence in the presence of the substrate fluorescein. Firefly luc mRNA is synthesized and purified by one-step transcription from a linear template using T7 transcriptase and LZCapAG(3'Acm) cap analogs. The mRNA will express luciferase protein when it enters the cell. Compared with the Cap0 structure and other commercially available Cap1 structures, the Cap1 structure of LZCapAG(3'Acm) enables more efficient and durable mRNA expression. LZCapAG(3'Acm) Firefly luc mRNA stock is suitable for mRNA delivery, translation efficiency, transfection efficiency and in vivo imaging experiments.

CAT: BRP-00455

Purity: ≥90% by HPLC

Description: Enhanced Green Fluorescent Protein (eGFP) is an enhanced variant of wild-type GFP with a maximum excitation/emission wavelength of 488 nm/509 nm, respectively. eGFP, as a common reporter molecule, emits a strong green fluorescence when excited and can be detected by fluorescence microscopy or flow cytometry. eGFP mRNA was synthesized and purified from linear template one-step transcription by T7 transcriptase and LZCapAG cap analogs. The Cap1 structure of LZCapAG(3'Acm) enables more efficient and durable mRNA expression compared to the Cap0 structure and other commercially available Cap1 structures. When this mRNA enters the cell, it will express a green fluorescent protein. eGFP mRNA stock is suitable for mRNA delivery, translation efficiency, transfection efficiency and in vivo imaging experiments.

CAT: BRP-00456

Purity: ≥90% by HPLC

Description: Red fluorescent protein (RFP) is a protein isolated from the Pacific Ocean sea anemone associated Discosomasp that can emit red fluorescence under ultraviolet irradiation at a wavelength of 587 nm. LZCapAG(3'Acm) RFP mRNA was synthesized and purified from linear template one-step transcription by T7 transcriptase and LZCapAG(3'Acm). Compared with the Cap0 structure and other commercially available Cap1 structures, the Cap1 structure of LZCapAG(3'Acm) enables more efficient and durable mRNA expression. LZCapAG(3'Acm) RFP mRNA has poly A and optimized 5 'UTR and 3' UTR structure, which can improve mRNA stability and promote mRNA translation to achieve better expression effect. The immunogenicity of mRNA can be effectively reduced after m1Ψ modification. LZCapAG(3'Acm) RFP mRNA stock is suitable for mRNA delivery, translation efficiency, transfection efficiency and in vivo imaging experiments.

CAT: BRP-00457

Purity: ≥90% by HPLC

Description: The excitation/emission wavelengths of mCherry red fluorescent protein were 587 nm/610 nm, respectively. LZCapAG(3'Acm) mCherry mRNA was synthesized and purified by one-step transcription of T7 transcriptase and LZCapAG(3'Acm) cap analogs from a linear template. After co-transcription, LZCapAG(3'Acm) generates mRNA with Cap1 structure. Compared with the Cap0 structure and other commercially available Cap1 structures, the Cap1 structure of LZCapAG(3'Acm) has a more efficient and durable expression. LZCapAG(3'Acm) mCherry mRNA has poly A and optimized 5 'UTR and 3' UTR structure, which can improve mRNA stability and promote mRNA translation to achieve better expression effect. The immunogenicity of mRNA can be effectively reduced after m1Ψ modification. LZCapAG(3'Acm) mCherry mRNA will express red fluorescent protein after entering cells, which is suitable for mRNA delivery, translation efficiency, transfection efficiency and in vivo imaging experiments.

CAT: BRP-00458

Purity: ≥90% by HPLC

Description: Renilla luciferase (RLuc) is a new type of luciferase with a size of 36 kD. The enzyme can catalyze enterocelins that can penetrate cell membranes under oxygen and emit fluorescence (maximum wavelength 480 nm), the reaction is so efficient that almost all of the energy input to the reaction is converted into light. LZCapAG(3'Acm) RLuc mRNA was synthesized and purified from linear template one-step transcription by T7 transcriptase and LZCapAG(3'Acm) cap analogs. The Cap1 structure of LZCapAG(3'Acm) enables more efficient and durable mRNA expression compared to the Cap0 structure and other commercially available Cap1 structures. LZCapAG(3'Acm) RLuc mRNA has a poly A and an optimized 5 'UTR and 3' UTR structure, which can improve mRNA stability and promote mRNA translation for better expression. LZCapAG(3'Acm) RLuc mRNA stock is suitable for mRNA delivery, translation efficiency, transfection efficiency and in vivo imaging experiments.

CAT: BRP-00459

Purity: ≥90% by HPLC

Description: Gaussia Luciferase (GLuc) is the smallest (20 kD) naturally secreted luciferase known in vivo. When using Gaussia Luciferase to perform fluorescence report experiment, the cell supernatant can be directly used for detection. The luciferase catalyzes the oxidation of the substrate coelenterin and emits light (480 nm) without the involvement of ATP. LZCapAG(3'Acm) GLuc mRNA was synthesized and purified from linear template one-step transcription by T7 transcriptase and LZCapAG(3'Acm) cap analogs. Compared with the Cap0 structure and other commercially available Cap1 structures, the Cap1 structure of LZCapAG(3'Acm) enables more efficient and durable mRNA expression. LZCapAG(3'Acm) GLuc mRNA has poly A and optimized 5 'UTR and 3' UTR structure, which can improve mRNA stability and promote mRNA translation to achieve a better expression effect. LZCapAG(3'Acm) GLuc mRNA is suitable for mRNA delivery, translation efficiency and transfection efficiency experiments.

CAT: BRP-00460

Purity: ≥90% by HPLC

Description: Under the guidance of sgRNA, Cas9 protein can introduce DNA double-strand breaks (DSBS) at designated genomic locations, and cells will then activate endogenous DNA repair programs, namely non-homologous end junction (NHEJ) or homologous recombination repair mechanism (HDR), to repair the target DSB, and realize the recognition and cutting of genomic DNA targets. LZCapAG(3'Acm) Cas9 mRNA can be used with purified sgRNA to perform the cutting function with sgRNA through the expressed Cas9 protein for CRISPR/Cas genome editing. LZCapAG(3'Acm) Cas9 mRNA was synthesized and purified from linear template one-step transcription by T7 transcriptase and LZCapAG(3'Acm) cap analogs. Compared with the Cap0 structure and other commercially available Cap1 structures, the Cap1 structure of LZCapAG(3'Acm) enables more efficient and durable mRNA expression. LZCapAG(3'Acm) Cas9 mRNA has poly A and optimized 5 'UTR and 3' UTR structure, which can improve mRNA stability and promote mRNA translation to achieve a better expression effect. LZCapAG(3'Acm) Cas9 mRNA stock is suitable for mRNA delivery, translation efficiency, transfection efficiency and gene editing experiments.

CAT: BRP-00461

Purity: ≥90% by HPLC