CPGs for oligo synthesis

5'-DMT-T-Suc-CPG; 500 Å (BRP-02291)

Description: 5'-DMT-T-Suc-CPG is used to add unmodified dT to the 3' end of oligonucleotides.
CAT: BRP-02291
Appearance: White powder
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Storage: Store at 2-8 °C
Shipping: Room temperature.
Cleavage Conditions: Use concentrated ammonia for 90 min at 25°C or 30 min at 60°C, or 1:1 ammonia:methylamine (AMA) for 25 min at 25°C when using fast deprotecting amidites.
Deprotection Conditions: When using fast deprotecting amidites (e.g., C-Ac; G-DMF; G-PAC), please use concentrated ammonia for 1h or AMA for 30 min at 60°C. When using standard amidites (e.g., C-Bz; G-iBu), please use concentrated ammonia for 5h at 60°C.
Synonyms: 5'-DMT-dT-Suc-CPG; 500 Å; 5'-O-DMT-2'-deoxy-T-3'-O-Suc-CPG; 500 Å

5'-DMT-T-Suc-CPG; 500 Å (High load) (BRP-02292)

Description: 5'-DMT-T-Suc-CPG is used to add unmodified dT to the 3' end of oligonucleotides.
CAT: BRP-02292
Appearance: White powder
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Storage: Store at 2-8 °C
Shipping: Room temperature.
Cleavage Conditions: Use concentrated ammonia for 90 min at 25°C or 30 min at 60°C, or 1:1 ammonia:methylamine (AMA) for 25 min at 25°C when using fast deprotecting amidites.
Deprotection Conditions: When using fast deprotection amidites (such as C-Ac; G-DMF), use concentrated ammonia at 60°C for 1h or AMA for 30 min. When using standard amidites (such as C-Bz; G-iBu), please use concentrated ammonia at 60°C for 5h.
Synonyms: 5'-DMT-dT-Suc-CPG; 500 Å (High load); 5'-O-DMT-2'-deoxy-T-3'-O-Suc-CPG; 500 Å (High load)

5'-DMT-U-Suc-CPG; 1000 Å (RNA) (BRP-02293)

Description: 5'-DMT-U-Suc-CPG; 1000 Å (RNA) is a reagent used in the solid-phase synthesis of RNA oligonucleotides for incorporating uridine (U) residues. It features a 5'-DMT (dimethoxytrityl) protective group to protect the 5' end of the uridine base, and a succinyl (Suc) linker that attaches the nucleoside to controlled pore glass (CPG) with a 1000 Å pore size. This setup facilitates efficient incorporation of uridine into RNA sequences while ensuring effective protection and stability during synthesis.
CAT: BRP-02293
Appearance: White powder
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Storage: Store at -20 °C
Shipping: Room temperature.
Cleavage Conditions: Use concentrated ammonia for 90 min at 25°C or 30 min at 60°C, or 1:1 ammonia:methylamine (AMA) for 25 min at 25°C when using fast deprotecting amidites.
Deprotection Conditions: When using fast deprotection amidites (such as C-Ac; G-DMF), use concentrated ammonia at 60°C for 1h or AMA for 30 min. When using standard amidites (such as C-Bz; G-iBu), please use concentrated ammonia at 60°C for 5h.
Synonyms: 5'-O-DMT-2'-OAc-U-3'-O-Suc-CPG; 1000 Å (RNA)

5'-DMT-U-Suc-CPG; 500 Å (RNA) (BRP-02294)

Description: 5'-DMT-U-Suc-CPG; 500 Å (RNA) is a reagent used in the solid-phase synthesis of RNA oligonucleotides for incorporating uridine (U) residues. It features a 5'-DMT (dimethoxytrityl) protective group to protect the 5' end of the uridine base, and a succinyl (Suc) linker that attaches the nucleoside to controlled pore glass (CPG) with a 500 Å pore size. This setup facilitates efficient incorporation of uridine into RNA sequences while ensuring effective protection and stability during synthesis.
CAT: BRP-02294
Appearance: White powder
Size Price Stock Quantity
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Storage: Store at -20 °C
Shipping: Room temperature.
Cleavage Conditions: Use concentrated ammonia for 90 min at 25°C or 30 min at 60°C, or 1:1 ammonia:methylamine (AMA) for 25 min at 25°C when using fast deprotecting amidites.
Deprotection Conditions: When using fast deprotecting amidites (e.g., C-Ac; G-DMF; G-PAC), please use concentrated ammonia for 1h or AMA for 30 min at 60°C. When using standard amidites (e.g., C-Bz; G-iBu), please use concentrated ammonia for 5h at 60°C.
Synonyms: 5'-O-DMT-2'-OAc-U-3'-O-Suc-CPG; 500 Å (RNA)

Aminopropyl-CPG; 1000 Å (BRP-02295)

Description: Aminopropyl CPG can be used for functionalization of base or modifier.
CAT: BRP-02295
Appearance: White powder
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Storage: Store at RT
Shipping: Room temperature.

Aminopropyl-CPG; 1400 Å (BRP-02296)

Description: Aminopropyl CPG can be used for functionalization of base or modifier.
CAT: BRP-02296
Appearance: White powder
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Storage: Store at RT
Shipping: Room temperature.

Aminopropyl-CPG; 2000 Å (BRP-02297)

Description: Aminopropyl CPG can be used for functionalization of base or modifier.
CAT: BRP-02297
Appearance: White powder
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Storage: Store at RT
Shipping: Room temperature.

Aminopropyl-CPG; 300 Å (BRP-02298)

Description: Aminopropyl CPG can be used for functionalization of base or modifier.
CAT: BRP-02298
Appearance: White powder
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Storage: Store at RT
Shipping: Room temperature.

Aminopropyl-CPG; 500 Å (BRP-02299)

Description: Aminopropyl CPG can be used for functionalization of base or modifier.
CAT: BRP-02299
Appearance: White powder
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Storage: Store at RT
Shipping: Room temperature.

BHQ-0 CPG; Glycolate, 1000 Å (BRP-02300)

Description: BHQ-0 CPG; Glycolate, 1000 Å can be used to add the non-fluorescent quencher BHQ-0 dye to the 3' end of oligonucleotides.
CAT: BRP-02300
Appearance: Orange powder
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Storage: Store at -20 °C
Shipping: Room temperature.
Cleavage Conditions: When using fast deprotecting amidites use concentrated Ammonia for 45 min at 25 °C or Ammonia/ Methylamine (AMA) (1:1) for 15 min at 25 °C. When using standard amidites (eg. C-Bz, G-iBu) use concentrated Ammonia for 45 min at 25 °C.
Deprotection Conditions: When using fast deprotecting amidites (e.g., C-Ac; G-DMF; G-PAC), please use concentrated ammonia for 1h or AMA for 30 min at 60°C. When using standard amidites (e.g., C-Bz; G-iBu), please use concentrated ammonia for 5h at 60°C.
Absorption Maximum (Lambda Max): 493

BHQ-0 CPG; Glycolate, 500 Å (BRP-02301)

Description: BHQ-0 CPG; Glycolate, 500 Å can be used to add the non-fluorescent quencher BHQ-0 dye to the 3' end of oligonucleotides.
CAT: BRP-02301
Appearance: Orange powder
Size Price Stock Quantity
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Storage: Store at -20 °C
Shipping: Room temperature.
Cleavage Conditions: When using fast deprotecting amidites use concentrated Ammonia for 45 min at 25 °C or Ammonia/ Methylamine (AMA) (1:1) for 15 min at 25 °C. When using standard amidites (eg. C-Bz, G-iBu) use concentrated Ammonia for 45 min at 25 °C.
Deprotection Conditions: When using fast deprotecting amidites (e.g., C-Ac; G-DMF; G-PAC), please use concentrated ammonia for 1h or AMA for 30 min at 60°C. When using standard amidites (e.g., C-Bz; G-iBu), please use concentrated ammonia for 5h at 60°C.
Absorption Maximum (Lambda Max): 493

BHQ-1 CPG; Glycolate, 1000 Å (BRP-02302)

Description: BHQ-1 CPG; Glycolate, 1000 Å can be used to add the non-fluorescent quencher BHQ-1 dye to the 3' end of oligonucleotides.
CAT: BRP-02302
Appearance: Dark red powder
Size Price Stock Quantity
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Storage: Store at -20 °C
Shipping: Room temperature.
Cleavage Conditions: When using fast deprotecting amidites use concentrated Ammonia for 45 min at 25 °C or Ammonia/ Methylamine (AMA) (1:1) for 15 min at 25 °C; or t-butylamine/H2O (1:3) for 90 min at 25 °C (TAMRA requires deprotection with TBA). When using standard amidites (eg. C-Bz, G-iBu) use concentrated Ammonia for 45 min at 25 °C.
Deprotection Conditions: When using fast deprotecting amidites (e.g., C-Ac; G-DMF; G-PAC), please use concentrated ammonia for 1h or AMA for 30 min at 60°C. When using standard amidites (e.g., C-Bz; G-iBu), please use concentrated ammonia for 5h at 60°C.
Absorption Maximum (Lambda Max): 534

BHQ-1 CPG; Glycolate, 500 Å (BRP-02303)

Description: BHQ-1 CPG; Glycolate, 500 Å can be used to add the non-fluorescent quencher BHQ-1 dye to the 3' end of oligonucleotides.
CAT: BRP-02303
Appearance: Dark red powder
Size Price Stock Quantity
-- -- --
Inquiry   
Storage: Store at -20 °C
Shipping: Room temperature.
Cleavage Conditions: When using fast deprotecting amidites use concentrated Ammonia for 45 min at 25 °C or Ammonia/ Methylamine (AMA) (1:1) for 15 min at 25 °C; or t-butylamine/H2O (1:3) for 90 min at 25 °C (TAMRA requires deprotection with TBA). When using standard amidites (eg. C-Bz, G-iBu) use concentrated Ammonia for 45 min at 25 °C.
Deprotection Conditions: When using fast deprotecting amidites (e.g., C-Ac; G-DMF; G-PAC), please use concentrated ammonia for 1h or AMA for 30 min at 60°C. When using standard amidites (e.g., C-Bz; G-iBu), please use concentrated ammonia for 5h at 60°C.
Absorption Maximum (Lambda Max): 534

BHQ-1 T Linker Arm, CPG; 500 Å (BRP-02304)

Description: BHQ-1 T Linker Arm, CPG is used for oligonucleotides labeled with 3-BHQ-1 dT.
CAT: BRP-02304
Appearance: Dark red powder
Size Price Stock Quantity
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Storage: Store at -20 °C
Shipping: Room temperature.
Cleavage Conditions: When using fast deprotecting amidites use concentrated Ammonia for 20 min at 25 °C or Ammonia/ Methylamine (AMA) (1:1) for 5 min at 25 °C; or t-butylamine/H2O (1:3) for 45 min at 25 °C (TAMRA requires deprotection with TBA). When using standard amidites (eg. C-Bz, G-iBu) use concentrated Ammonia for 20 min at 25 °C.
Deprotection Conditions: When using fast deprotection amidites (such as C-Ac; G-DMF), use concentrated ammonia at 60°C for 1h or AMA for 30 min. When using standard amidites (such as C-Bz; G-iBu), please use concentrated ammonia at 60°C for 5h.
Absorption Maximum (Lambda Max): 548

BHQ-2 CPG; Glycolate, 1000 Å (BRP-02305)

Description: BHQ-2 CPG; Glycolate is used to add the non-fluorescent quencher BHQ-2 to the 3' end of oligonucleotides.
CAT: BRP-02305
Appearance: Purple powder
Size Price Stock Quantity
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Inquiry   
Storage: Store at -20 °C
Shipping: Room temperature.
Cleavage Conditions: When using fast deprotecting amidites use concentrated Ammonia for 45 min at 25 °C or Ammonia/ Methylamine (AMA) (1:1) for 15 min at 25 °C; or t-butylamine/H2O (1:3) for 90 min at 25 °C (TAMRA requires deprotection with TBA). When using standard amidites (eg. C-Bz, G-iBu) use concentrated Ammonia for 45 min at 25 °C.
Deprotection Conditions: When using fast deprotecting amidites (e.g., C-Ac; G-DMF; G-PAC), please use concentrated ammonia for 1h or AMA for 30 min at 60°C. When using standard amidites (e.g., C-Bz; G-iBu), please use concentrated ammonia for 5h at 60°C.
Absorption Maximum (Lambda Max): 579

BHQ-2 CPG; Glycolate, 500 Å (BRP-02306)

Description: BHQ-2 CPG; Glycolate is used to add the non-fluorescent quencher BHQ-2 to the 3' end of oligonucleotides.
CAT: BRP-02306
Appearance: Purple powder
Size Price Stock Quantity
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Inquiry   
Storage: Store at -20 °C
Shipping: Room temperature.
Cleavage Conditions: When using fast deprotecting amidites use concentrated Ammonia for 45 min at 25 °C or Ammonia/ Methylamine (AMA) (1:1) for 15 min at 25 °C; or t-butylamine/H2O (1:3) for 90 min at 25 °C (TAMRA requires deprotection with TBA). When using standard amidites (eg. C-Bz, G-iBu) use concentrated Ammonia for 45 min at 25 °C.
Deprotection Conditions: When using fast deprotecting amidites (e.g., C-Ac; G-DMF; G-PAC), please use concentrated ammonia for 1h or AMA for 30 min at 60°C. When using standard amidites (e.g., C-Bz; G-iBu), please use concentrated ammonia for 5h at 60°C.
Absorption Maximum (Lambda Max): 579

BHQ-2 T Linker Arm, CPG; 500 Å (BRP-02307)

Description: BHQ-2 T Linker Arm, CPG is used for oligonucleotides labeled with 3-BHQ-2 dT.
CAT: BRP-02307
Appearance: Dark purple powder
Size Price Stock Quantity
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Storage: Store at -20 °C
Shipping: Room temperature.
Cleavage Conditions: When using fast deprotecting amidites use concentrated Ammonia for 20 min at 25 °C or Ammonia/ Methylamine (AMA) (1:1)for 5 min at 25 °C; or t-butylamine/H2O(1:3) for 45 min at 25 °C (TAMRA requires deprotection with TBA). When using standard amidites (eg. C-Bz, G-iBu) use concentrated Ammonia for 20 min at 25 °C.
Deprotection Conditions: When using fast deprotection amidites (such as C-Ac; G-DMF), use concentrated ammonia at 60°C for 1h or AMA for 30 min. When using standard amidites (such as C-Bz; G-iBu), please use concentrated ammonia at 60°C for 5h.
Absorption Maximum (Lambda Max): 592

BHQ-3 CPG; Glycolate, 1000 Å (BRP-02308)

Description: BHQ-3 CPG; Glycolate is used to add non-fluorescent quencher BHQ-3 to the 3' end of oligonucleotides.
CAT: BRP-02308
Appearance: Dark blue powder
Size Price Stock Quantity
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Storage: Store at -20 °C
Shipping: Room temperature.
Synthesis Conditions: We recommend only using fast deprotecting amidites with this product due to the mild cleavage and deprotection conditions.
Deprotection Conditions: Cleave and deprotect in concentrated NH4OH for 1 hour at 60°C (after 1 hour at 60°C; degradation of product may occur).
Absorption Maximum (Lambda Max): 672

C16 CPG; 1000 Å (BRP-02309)

Description: C16 CPG is used to incorporate a C16 spacer at the 3' end of the oligonucleotide.
CAT: BRP-02309
Appearance: White to off-white powder
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Storage: Store at -20 °C
Shipping: Room temperature.
Synthesis Conditions: Standard synthesis conditions
Cleavage Conditions: Use concentrated ammonia for 45 minutes at 25 °C or 1:1 ammonia:methylamine (AMA) for 15 minutes at 25 °C.
Synonyms: C16 CPG (1-DMT-2-Hexadecyl-Glyc-CPG); 1000 Å

C2 CPG; 500 Å (BRP-02310)

Description: C2 CPG is used to add C2 spacers to the 3' end of oligonucleotides.
CAT: BRP-02310
Appearance: White to off-white powder
Size Price Stock Quantity
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Storage: Store at 2-8 °C
Shipping: Room temperature.
Cleavage Conditions: Use concentrated ammonia for 45 minutes at 25 °C or 1:1 ammonia:methylamine (AMA) for 15 minutes at 25 °C.
Deprotection Conditions: When using fast deprotecting amidites (e.g., C-Ac; G-DMF; G-PAC), please use concentrated ammonia for 1h or AMA for 30 min at 60°C. When using standard amidites (e.g., C-Bz; G-iBu), please use concentrated ammonia for 5h at 60°C.
Synonyms: C2 CPG (DMT-1,2-Etheyleneglycol-Suc-CPG); 500 Å

What are CPGs?

Controlled Pore Glasses (CPGs) are made of silica and are widely used in solid phase synthesis reactions of oligonucleotides. CPG spheres have many irregular pore channels and are pore size stable. For spatial reasons, large pore sizes are suitable for the synthesis of long fragments, and short pore sizes are suitable for the synthesis of short fragments. BOC Sciences offers a wide range of CPGs for oligonucleotide synthesis to choose from, with pore sizes ranging from 300 Å-5000 Å. Groups linked to CPGs include TAMRA, DMT-Phosphate, Fluorescein, Aminopropyl, etc.

Different pore size CPGs can be used to synthesize oligonucleotide products with different loadings:

(1) 500 Å/600 Å pore size CPG products are suitable for oligonucleotide synthesis of < 35 mers. For example, therapeutic oligonucleotides can be synthesized at loads up to 100 μmol/g.

(2) 1000 Å pore size CPG products for >35 mers or highly modified oligonucleotide synthesis.

(3) 2000 Å/3000 Å pore size CPG products, suitable for oligonucleotide synthesis on a scale of 80 mers or more, with synthetic loads typically ranging from 10 to 20 μmol/g.

Examples of CPGs

Because tetramethylrhodamine (TAMRA) is not base-stable, procedures for cleaving and deprotecting labeled oligonucleotides must be carefully considered. For example, to simplify the preparation of TAMRA oligonucleotides, 3'-TAMRA CPG for 3' labeling and TAMRA-dT for in-sequence labeling are available.

Fluorescein CPG is traditionally used to add fluorescein labeling to the 3'-end. For example, 3'-(6-FAM) CPG effectively blocks polymerase extension and exonuclease digestion at the 3'-end.

The phosphate functional group on the surface of DMT-Phosphate-CPG usually carries a DMT protecting group. DMT is a commonly used protecting group that protects the phosphate group from non-specific reactions during nucleotide synthesis. The DMT protecting group can be easily removed at the end of the synthesis to expose the phosphate group for further modification. CPGs have a pore size and pore structure that can be tuned similar to that of conventional CPGs. This controllable pore size facilitates efficient solid-phase synthesis of oligonucleotides and ensures good attachment and stability of the phosphate functional groups.

Aminopropyl-CPG is a CPG modified by an aminopropyl functional group, and we can provide products with pore sizes ranging from 300 Å-2000 Å. The products can be used for the amination modification of nucleotides, and by introducing an amino functional group, the chemistry and reactivity of nucleotides can be extended.

Features of CPGs

The pore size and pore structure of CPGs can be tuned as needed, typically in the range of 3-30 nanometers. This controllable pore size and pore structure helps ensure efficient solid phase synthesis of oligonucleotides.

CPGs have a large amount of surface activity, providing enough binding sites to allow nucleotide immobilization and chemical reactions to take place during the synthesis process.

CPGs have good chemical stability and can be used under common nucleotide synthesis reaction conditions. They can tolerate synthesis conditions such as alkaline conditions, organic solvents, and high temperatures, thus ensuring high-quality oligonucleotide synthesis.

CPGs can be scaled and adjusted as needed for both small-scale research and large-scale industrial production.

After years of development, BOC Sciences has the expertise and experience to help you synthesize high-quality CPGs for oligo synthesis and accelerate the achievement of your research goals.

If you have any special requirements for CPGs products, please feel free to contact us. We look forward to working with you on attractive projects.

* Only for research. Not suitable for any diagnostic or therapeutic use.
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