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CPGs for oligo synthesis

DMT-dT-LCAA-CPG; 500 Å (BRP-02343)

Description: DMT-dT-LCAA-CPG; 500 Å is a reagent used in the solid-phase synthesis of oligonucleotides for incorporating thymidine (dT) residues. It features a 5'-DMT (dimethoxytrityl) protective group, which safeguards the 5' end during synthesis, and is attached to a long-chain alkylamine (LCAA) linker bound to controlled pore glass (CPG) with a 500 Å pore size. This setup is designed for efficient synthesis of DNA sequences, particularly shorter oligonucleotides, allowing for precise thymidine incorporation with stable protection throughout the synthesis process.
CAT: BRP-02343
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Synonyms: DMT-dT-LCAA-CPG; DMT dT LCAA CPG

U-CPG; 1000 Å (BRP-02344)

Description: CPG column for incorporation of unmodified U at the 3' end of an oligonucleotide.
CAT: BRP-02344
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Synonyms: 5'-DimethoxytritylUridine, 3'-succinoyl-long chain alkylamino-CPG 1000; U CPG Column; 5'-O-DMT-2'-O-suc-U-3'-suc-CPG 1000 Å; DMT-2'-O-suc-U-suc-CPG 1000 Å

U-CPG; 5000 Å (BRP-02345)

Description: CPG column for incorporation of unmodified U at the 3' end of an oligonucleotide.
CAT: BRP-02345
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Synonyms: 5'-DimethoxytritylUridine, 3'-succinoyl-long chain alkylamino-CPG 5000; U CPG Column; 5'-O-DMT-2'-O-suc-U-3'-suc-CPG 5000 Å; DMT-2'-O-suc-U-suc-CPG 5000 Å

GalNAc(TEG)-CPG

Description: GalNAc CPG allows the synthesis of siRNA-GalNAc conjugate.
CAT: BRP-02362
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3'-6-Fluorescein Serinol CPG

Description: 3'-6-Fluorescein Serinol CPG is a key constituent in the realm of biomedical science, holding immense significance for the research and development of biocompatible oligonucleotides. Serving as an indispensable companion, it orchestrates the solid-phase research and development process owing to its inherent nature as a controlled pore glass support. By facilitating seamless labeling and detection of fluorescence, this remarkable entity empowers researchers to fashion nucleotide probes instrumental in unraveling the intricacies of gene expression analysis, genetic sequencing, and the diagnosis of maladies like cancer and infectious disorders.
CAT: BRP-02363
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Storage: Store at -20 °C
Synonyms: 3-Dimethoxytrityloxy-2-(3-(6-carboxy-(di-O-pivaloyl-fluorescein)propanamido)propyl)-1-O-succinyl-long chain alkylamino-CPG; 3'-6-Fluorescein Serinol CPG (1000Å)

GalNAc-EDTAECA-PEG2 CPG

Description: It is a diamine-scaffold-based GalNAc CPG that plays a key role in the synthesis of N-acetylgalactosamine (GalNAc)-siRNA conjugate as a solid support to facilitate the step-up synthesis of amide-phosphorus. This method guarantees the high purity and yield of the final product, while improving the stability of the synthesized siRNA molecule. In addition, it allows for improved liver-targeted delivery of siRNA by attaching GalNAc, thereby increasing cellular uptake efficiency and thus improving therapeutic efficacy.
CAT: BRP-02500
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Synonyms: GalNAc-EDT-(2-AECA)-PEG2 CPG; Peracetylated-TG2-DMT-Suc CPG; Peracetylated-TG2 CPG; Tri-TG2(OAc)3-DMT-Suc CPG; Tri-Gal(OAc)3 TG1-DMT-Suc CPG

What are CPGs?

Controlled Pore Glasses (CPGs) are made of silica and are widely used in solid phase synthesis reactions of oligonucleotides. CPG spheres have many irregular pore channels and are pore size stable. For spatial reasons, large pore sizes are suitable for the synthesis of long fragments, and short pore sizes are suitable for the synthesis of short fragments. BOC Sciences offers a wide range of CPGs for oligonucleotide synthesis to choose from, with pore sizes ranging from 300 Å-5000 Å. Groups linked to CPGs include TAMRA, DMT-Phosphate, Fluorescein, Aminopropyl, etc.

Different pore size CPGs can be used to synthesize oligonucleotide products with different loadings:

(1) 500 Å/600 Å pore size CPG products are suitable for oligonucleotide synthesis of < 35 mers. For example, therapeutic oligonucleotides can be synthesized at loads up to 100 μmol/g.

(2) 1000 Å pore size CPG products for >35 mers or highly modified oligonucleotide synthesis.

(3) 2000 Å/3000 Å pore size CPG products, suitable for oligonucleotide synthesis on a scale of 80 mers or more, with synthetic loads typically ranging from 10 to 20 μmol/g.

Examples of CPGs

Because tetramethylrhodamine (TAMRA) is not base-stable, procedures for cleaving and deprotecting labeled oligonucleotides must be carefully considered. For example, to simplify the preparation of TAMRA oligonucleotides, 3'-TAMRA CPG for 3' labeling and TAMRA-dT for in-sequence labeling are available.

Fluorescein CPG is traditionally used to add fluorescein labeling to the 3'-end. For example, 3'-(6-FAM) CPG effectively blocks polymerase extension and exonuclease digestion at the 3'-end.

The phosphate functional group on the surface of DMT-Phosphate-CPG usually carries a DMT protecting group. DMT is a commonly used protecting group that protects the phosphate group from non-specific reactions during nucleotide synthesis. The DMT protecting group can be easily removed at the end of the synthesis to expose the phosphate group for further modification. CPGs have a pore size and pore structure that can be tuned similar to that of conventional CPGs. This controllable pore size facilitates efficient solid-phase synthesis of oligonucleotides and ensures good attachment and stability of the phosphate functional groups.

Aminopropyl-CPG is a CPG modified by an aminopropyl functional group, and we can provide products with pore sizes ranging from 300 Å-2000 Å. The products can be used for the amination modification of nucleotides, and by introducing an amino functional group, the chemistry and reactivity of nucleotides can be extended.

Features of CPGs

The pore size and pore structure of CPGs can be tuned as needed, typically in the range of 3-30 nanometers. This controllable pore size and pore structure helps ensure efficient solid phase synthesis of oligonucleotides.

CPGs have a large amount of surface activity, providing enough binding sites to allow nucleotide immobilization and chemical reactions to take place during the synthesis process.

CPGs have good chemical stability and can be used under common nucleotide synthesis reaction conditions. They can tolerate synthesis conditions such as alkaline conditions, organic solvents, and high temperatures, thus ensuring high-quality oligonucleotide synthesis.

CPGs can be scaled and adjusted as needed for both small-scale research and large-scale industrial production.

After years of development, BOC Sciences has the expertise and experience to help you synthesize high-quality CPGs for oligo synthesis and accelerate the achievement of your research goals.

If you have any special requirements for CPGs products, please feel free to contact us. We look forward to working with you on attractive projects.

* Only for research. Not suitable for any diagnostic or therapeutic use.

Our Products

BOC Sciences is a leading provider of DNA and RNA products and services that meet the needs of researchers and pharmaceutical companies worldwide. Its broad product portfolio includes various types of oligonucleotides, DNA/RNA synthesis feedstocks, RNA interference tools, and advanced drug delivery systems, among others. Designed to promote cutting-edge research and innovative therapeutic solutions, at BOC Sciences you are sure to find the right product for you.

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