Oligo Synthesis Service

BOC RNA provides synthesis services for a wide range of oligonucleotides (DNA, RNA) and derivatives, including natural DNA, siRNA, mRNA, and DNA/RNA oligos, or non-natural and more stable oligonucleotides (GMP grade oligo and non-cGMP grade oligo). BOC RNA has been committed to providing high-quality oligo-nucleic acid synthesis and formulation services for global partners over the years.

Introduction to Oligo Synthesis Process

• What is Oligo Synthesis?

Oligo synthesis is the process of chemically synthesizing short DNA or RNA sequences. The synthesis is performed using a DNA synthesizer, which can generate the desired sequence by adding nucleotides sequentially, one after another. The process starts with a support that bears a protecting group on its 3'-OH group. The first nucleotide is introduced by adding the 5'-OH group to the support-bound 3'-OH group, followed by the deprotection of the 5'-OH group for the introduction of the next nucleotide.

• Steps Involved in Oligo Synthesis

The oligo synthesis process involves several steps.First, the protecting groups on the support-bound nucleotides are selectively removed by adding deprotection reagents. The nucleotides to be added in the next sequence are then coupled with the support-bound sequence, resulting in a growing chain. The coupling is usually carried out using phosphoramidite chemistry, which involves the activation of the incoming nucleotide by a phosphoramidite reagent. After coupling, the 5'-OH group of the newly introduced nucleotide is protected. The process is then repeated until the desired oligonucleotide sequence is obtained.

• Post Synthesis Modifications

After oligo synthesis, some downstream applications may require modifications to the oligonucleotide sequence. These modifications can include fluorophores, biotin, or other chemical modifications that can enhance the oligo's specificity and functionality. In some cases, oligonucleotides may need to be purified by methods such as high-performance liquid chromatography (HPLC) to remove truncated sequences, deletion sequences, or other impurities.

• Applications of Oligo Synthesis

Oligo synthesis has been an essential tool for numerous applications in molecular biological research. For example, oligonucleotides are used in PCR (polymerase chain reaction) to amplify DNA sequences, in gene expression analysis to study gene expression levels, in sequencing to read DNA sequences, and in gene editing to introduce specific mutations to the DNA sequence. Oligonucleotides have also been used as therapeutic agents for various diseases, including genetic disorders, viral infections, and cancer.

Contents of Oligo Synthesis Service

We can provide more than 40 types of chemical modifications and hundreds of common modifications to meet our customers’ sequence synthesis and modification requirements. We provide standard purification, PAGE purification, and HPLC purification from milligram level to kilogram level accordingly.

Oligo Synthesis Purification Methods

Why Work With Us

• Flexible synthesis specifications - Scales from 20 nmol to 10 µmol or more. Bulk orders are available upon request
• Comprehensive modifications & labeling - From general modifications to fluorescent/non-fluorescent labeling and even dual and multiple labeling
• Advanced high-throughput production equipment
• Experienced team - Scientists and staff with over 15 years of experience in oligo synthesis and modifications.
• Competitive price - Using internally produced raw materials (phosphoramidites, reagents, and solvents) combined with our automatic oligonucleotide synthesis and purification system, it can provide low-cost and high-quality oligonucleotides.
• Fast delivery
• A trusted and well-known contract manufacturing partner