Oligonucleotides—short, single- or double-stranded sequences of nucleotides—are indispensable tools in modern molecular biology, drug development, diagnostics, and synthetic biology. At BOC Sciences, we offer a full-spectrum portfolio of preclinical Oligonucleotides Services, encompassing synthesis, chemical modification, labeling, conjugation, purification, and advanced delivery formats. With over two decades of industry experience and a reputation for scientific excellence, BOC Sciences stands as a leading partner for customizable and scalable oligo solutions. Our services are precisely engineered to meet the stringent requirements of biotechnology, pharmaceutical R&D, and academic research worldwide.
BOC Sciences leverages an integrated and highly optimized platform for oligonucleotide synthesis, combining advanced automation, proprietary sensitivity-enhancing protocols, and next-generation solid-phase technologies. Our synthesis technology is engineered to address the increasing demand for precision, sequence fidelity, and scalability across DNA, RNA, and custom oligonucleotide constructs.
BOC Sciences employs phosphoramidite-based SPOS, the gold standard in oligonucleotide chemistry, with enhanced stepwise precision and process control to ensure batch-to-batch consistency and product integrity. Core components of our SPOS process include:
We source ultra-pure, contaminant-controlled nucleoside phosphoramidites to reduce synthesis noise and impurity load, especially critical for therapeutic preclinical development and sensitive bioassays.
Each synthesis run is executed on state-of-the-art synthesizers equipped with high-resolution delivery and wash cycles, programmable for specific synthesis scales (nmol to gram level), facilitating both discovery-phase and bulk research needs.
Our systems are calibrated to monitor every coupling, capping, oxidation, and deprotection step in real-time. Deviations trigger immediate cycle corrections or alerts, ensuring sequence fidelity and minimizing yield loss.
SPOS protocols at BOC Sciences are designed for seamless scalability. Whether synthesizing 20-mers for qPCR primers or long-chain 120-mers for siRNA precursors, our system supports flexible output formats—desalted, HPLC-purified, or lyophilized upon request.
The Sensitive DNA/RNA Synthesis Platform at BOC Sciences is an innovative technology designed for the synthesis of nucleic acid molecules with sensitive functional groups. This platform utilizes advanced techniques and mild synthesis conditions to ensure a more stable and efficient nucleic acid synthesis process. It is primarily used in cutting-edge fields such as RNA drugs, gene editing, vaccine development, and synthetic biology, providing strong technical support for researchers.
The platform uses Dim-Dmoc technology, enabling DNA/RNA synthesis under mild conditions (pH 4–8) without the use of strong bases or nucleophilic reagents, minimizing damage to functional groups.
Technology: It incorporates novel amino and phosphate protecting groups, offering enhanced protection for sensitive functional groups within nucleic acids during the synthesis process, thus improving the stability of the molecules.
The platform successfully introduces a variety of sensitive functional groups (such as alkyl halides, α-chloramides, sulfates, alkyl and aryl esters) into oligonucleotides, supporting the synthesis of complex structures.
It is applicable to RNA drug development, vaccine research, gene editing, targeted delivery systems, and synthetic biology, driving breakthroughs and applications in these fields.
By avoiding strong bases and nucleophilic reagents, the platform offers superior protection to sensitive functional groups, ensuring that they remain intact during synthesis and facilitating the development of stable, complex nucleic acid molecules.
BOC Sciences offers a high-precision Custom Oligo Synthesis service designed to support researchers in need of non-standard oligonucleotide constructs for advanced applications. Beyond conventional DNA or RNA sequences, we deliver synthetically engineered oligonucleotides that support novel assay formats, diagnostic tools, structural biology research, aptamer-based discovery, and high-throughput screening protocols. Our custom synthesis capabilities are backed by advanced automation, high-fidelity phosphoramidite chemistry, and rigorous QC procedures—ensuring reliable, reproducible, and scalable results from nanomole to multigram quantities.
For projects requiring stringent regulatory standards, BOC Sciences offers cGMP Oligonucleotide Manufacturing Services. These services are ideal for preclinical research and development. We adhere to Current Good Manufacturing Practice (cGMP) guidelines at every stage of production to ensure consistency and compliance with international standards. Our process is optimized for the manufacture of oligonucleotides used in preclinical trials, vaccine development, and gene therapies, ensuring the highest quality and safety for your therapeutic research needs.
Our Custom RNA Oligonucleotide Synthesis service supports a wide range of RNA-based applications, such as mRNA, siRNA, miRNA, and other non-coding RNAs. Whether you're designing small interfering RNAs for gene silencing or synthesizing mRNA for gene expression studies, our service guarantees high efficiency and high yield. We offer modifications such as 2'-OMe, 2'-F, LNA, and chemical stabilization to improve RNA stability and minimize degradation, ensuring reliability for in vitro and in vivo applications.
We specialize in DNA Oligonucleotide Synthesis, offering both standard and modified DNA oligos for a wide array of applications, including PCR, cloning, sequencing, and gene editing. From designing long oligos for complex genetic manipulations to providing primers for high-fidelity PCR, our synthesis service ensures your oligos are tailored to your exact needs. We also offer modifications such as fluorescent labeling, phosphorothioate linkages, and additional structural adjustments to improve hybridization, stability, and detection.
Antisense Oligonucleotides (ASOs) are critical tools in gene silencing and therapeutic research. Our Antisense Oligonucleotide Synthesis service is designed to create highly effective ASOs with modifications that enhance stability, cellular uptake, and binding affinity. This service is essential for applications such as gene therapy, cancer research, and neurological disorder treatments. With precision synthesis, you can target mRNA transcripts with exceptional specificity, reducing unwanted side effects and improving therapeutic outcomes.
Our Custom Aptamer Synthesis service offers the ability to create nucleic acid-based ligands that bind specifically to your target molecule, providing a powerful tool for diagnostics, therapeutics, and biosensor applications. Aptamers are ideal for use in a wide range of applications, including drug delivery systems, molecular diagnostics, and targeted therapies. We offer custom designs with modifications that enhance their affinity, specificity, and stability in biological environments.
Locked Nucleic Acids (LNAs) are modified nucleotides with a unique structure that enhances their hybridization properties. Our Custom LNA Synthesis service offers oligos that are ideal for high-precision assays, such as quantitative PCR, gene expression analysis, and in situ hybridization. LNAs are especially valuable when working with difficult targets, offering higher specificity and resistance to nucleases, making them essential for accurate detection and quantification.
Our Custom PCR Primer Synthesis service provides high-quality primers tailored to your PCR amplification needs. Whether you're conducting standard PCR, qPCR, or high-throughput sequencing, our primers are designed for optimal specificity, yield, and efficiency. We offer a wide range of modifications, such as fluorescent labels, phosphorothioate bonds, and 3' modifications, to suit your experimental requirements.
For complex projects that require gene synthesis, we offer a Gene Synthesis Service that enables the assembly of entire genes from scratch. Whether you need to clone a gene into a vector or express a synthetic gene for protein production, our gene synthesis process ensures that the synthesized sequence is accurate, error-free, and scalable. With advanced assembly technologies, we can deliver genes optimized for functional expression and high-throughput applications.
Our Diagnostic Probes & Oligonucleotides service is designed to support molecular diagnostics by providing high-specificity probes for detecting genetic markers. Whether you're working with PCR, FISH, or other diagnostic techniques, we offer oligos that are optimized for sensitivity, stability, and high performance. Fluorescent, biotinylated, or quenching modifications can be included to enhance detection and quantification in diagnostic applications.
Each of these services at BOC Sciences is tailored to ensure that your oligonucleotides meet the highest standards of quality and performance for your research needs. Whether you are involved in gene therapy, diagnostics, gene editing, or any cutting-edge research, we offer reliable, scientifically rigorous solutions that ensure your work can proceed with the utmost confidence.
Oligo specialists at BOC Sciences are fully equipped to offer you comprehensive and expert support.
Online InquiryOur oligonucleotide modification services are designed to enhance the stability, specificity, and performance of your oligos for various applications. Whether you're working with antisense oligonucleotides (ASOs), small interfering RNAs (siRNAs), or CRISPR guide RNAs, we provide a wide range of modification options to improve the oligo's pharmacokinetics and resistance to nuclease degradation. We offer backbone modifications such as phosphorothioate (PS), methylphosphonate, and boranophosphate, which increase the oligonucleotide's resistance to exonucleases and enhance cellular uptake. Additionally, we support sugar modifications, including 2'-OMe (2'-O-methyl), 2'-F (2'-fluoro), and Locked Nucleic Acids (LNA), which stabilize the oligonucleotide and improve hybridization with complementary sequences. For improved targeting, modifications such as biotinylation, fluorescence labeling, or the introduction of chemical groups that facilitate protein interactions can be incorporated to tailor oligos to your specific needs.
Our oligonucleotide conjugation services enable you to attach oligos to various biomolecules, which can be critical for applications like targeted drug delivery, biomarker detection, or in vivo tracking. We offer conjugation to a wide array of functional groups including fluorescent dyes (e.g., FAM, Cy3, Cy5), biotin for affinity binding, peptides for enhanced cell penetration (e.g., cell-penetrating peptides, RGD peptides), and antibodies for targeted delivery.Through our advanced coupling technologies, we ensure that the conjugation process preserves the biological activity of both the oligonucleotide and the attached molecule. Whether you are developing conjugates for imaging applications, functional assays, or drug delivery vehicles, we provide expert guidance and custom solutions to achieve the desired outcome.
Ensuring the purity and integrity of your oligonucleotides is essential for successful experimentation. At BOC Sciences, we offer comprehensive oligonucleotide analysis and purification services to ensure that every oligo meets the highest standards of quality. We use state-of-the-art analytical techniques such as High-Performance Liquid Chromatography (HPLC), Capillary Electrophoresis (CE), and Mass Spectrometry (MS) to assess the purity, size distribution, and sequence integrity of your oligos. Our purification methods include desalting, reverse-phase HPLC, and PAGE, which are selected based on your specific needs—whether you are working with a small batch or require large-scale purification. These rigorous methods allow us to provide you with oligos that have minimal sequence impurities, ensuring that they will perform reliably in your experiments.
Effective delivery of oligonucleotides to target cells or tissues is one of the key challenges in therapeutic and diagnostic applications. BOC Sciences offers specialized oligonucleotide delivery services to optimize the bioavailability and efficacy of your oligos in both in vitro and in vivo studies. We work with a variety of advanced delivery platforms including lipid nanoparticles (LNPs), polymer-based nanoparticles, and cationic lipids to ensure efficient cellular uptake of your oligonucleotides. Our team can also help optimize formulations to increase stability, reduce toxicity, and improve targeting efficiency. Whether you are developing siRNAs, antisense oligonucleotides (ASOs), or CRISPR guide RNAs, our delivery solutions are tailored to your project's specific requirements, ensuring that your oligos are delivered to the right cells with minimal degradation and off-target effects.
| Cationic Nanoemulsions | Cationic Polypeptides | Exosome | Liposomes |
| Lipid Nanoparticle | Polymer Complexes | siRNA Conjugates |
By offering these specialized services, BOC Sciences ensures that your oligonucleotide research is supported with the highest level of technical expertise and precision. Whether you need custom modifications, conjugation to biomolecules, rigorous purification, or advanced delivery solutions, our team is committed to helping you achieve your research goals efficiently and effectively. Our commitment to quality and flexibility makes us the ideal partner for your preclinical oligonucleotide projects.
At BOC Sciences, we prioritize a seamless and customer-centric process for all oligonucleotide service requests. From initial consultation to final delivery, every step is designed to meet the highest standards of precision and efficiency. Below is a detailed outline of how you can place and track an order with us:
Tip: If you're unsure about which modifications or synthesis scale to choose, we offer a pre-order consultation to optimize the design based on your application.
Once we have the sequence and any modification preferences, we will work with you to define the final order specifications. Key details include:
Tip: For custom conjugates, such as oligos with peptides or lipids, discuss your intended application to ensure we use the correct linker chemistry.
After gathering all necessary details, we will send you a formal confirmation of the order, including the following:
At this point, you can either confirm the order or request any adjustments. If changes are needed, we'll ensure you are promptly updated with the revised specifications.
Once confirmed, our state-of-the-art synthesis platform begins the production of your oligonucleotide. This phase involves multiple in-process quality control steps to ensure high fidelity:
Tip: If you have high sensitivity requirements (e.g., low GC content or complex sequences), let us know in advance so we can apply specialized synthesis protocols to ensure success.
Once the oligonucleotide is synthesized, we move on to purification and thorough analytical testing:
This stage ensures that the oligonucleotide meets the exact specifications you provided.
After the oligonucleotides are purified and tested, we prepare a Certificate of Analysis (COA) for your order. The COA includes:
You will receive this documentation along with your order, ensuring transparency and confidence in the quality of your oligos.
Once the oligonucleotides pass QC and the COA is finalized, the product is carefully packaged for shipment:
Tip: If your experiment is time-sensitive, we offer expedited shipping options to ensure faster delivery.
Once your order is received, our support doesn't end. Should you need further assistance, our team remains available for:
We're committed to supporting your research throughout the life cycle of your project.
By choosing BOC Sciences, you gain access to an oligonucleotide synthesis service built for speed, reliability, and precision, with expert scientific support at every stage.
At BOC Sciences, we distinguish ourselves through a combination of scientific excellence, technical expertise, and unwavering commitment to customer satisfaction. Below are the key reasons why our oligonucleotide services are trusted by researchers across the globe:
We provide high-quality oligonucleotides that meet the most stringent standards. From initial synthesis through to final delivery, every batch undergoes rigorous quality control checks. Our analytical techniques include high-resolution capillary electrophoresis (CE), mass spectrometry (MS), and UV spectroscopy to ensure your oligos are of the highest purity, sequence accuracy, and functionality.
We recognize that each research project is unique, which is why we offer an extensive range of customization options. Whether you need standard oligos or highly specialized sequences with complex modifications, we have the capabilities to meet your precise requirements. From small-scale (for pilot studies) to large-scale synthesis (for industrial applications), we adapt to the scale that suits your needs.
Our team of highly trained experts is available at every step of the process to provide scientific support. Whether you require help in sequence design, synthesis optimization, or troubleshooting, our experts are at hand to guide you. We provide expert advice on selecting the appropriate modifications for your oligos to enhance stability, binding efficiency, and specific activity. From sequence submission to post-delivery troubleshooting, our team ensures the success of your project.
With a global customer base and local expertise, BOC Sciences is uniquely positioned to serve researchers across the world. Our logistics and shipping capabilities ensure that your oligonucleotide order reaches you quickly, no matter where you are located. We serve researchers from across the globe, ensuring that the complexities of international shipping are never an obstacle to receiving the products you need. Our team has deep knowledge of regional scientific needs, allowing us to provide more precise and applicable advice to our clients.
Oligonucleotides have become indispensable tools across a wide array of research areas due to their versatility and the precision with which they can be synthesized and modified. At BOC Sciences, our oligonucleotide services cater to the diverse needs of scientific disciplines, from basic molecular biology to advanced biotechnology. Below are some of the key applications for our oligonucleotides services:
Oligonucleotides are essential components in the synthesis and cloning of genes for various applications, including functional studies and protein expression. By designing specific primers and gene fragments, researchers can:
The high accuracy and customization options of our oligonucleotide services ensure the success of gene synthesis, enabling researchers to explore novel gene sequences and proteins.
PCR is a foundational technique in molecular biology, and oligonucleotides are the core elements that drive the amplification process. With our oligonucleotide services, you can optimize PCR primers to suit your specific application needs:
Our high-quality primers and probes contribute to the efficiency, sensitivity, and reproducibility of PCR experiments, even with complex or low-abundance targets.
The CRISPR-Cas9 system has revolutionized gene editing, and oligonucleotides play a critical role in both the design and execution of CRISPR experiments. Our services support various stages of CRISPR-based research:
With our expertise in CRISPR technology, we enable researchers to achieve precise gene editing with minimal off-target effects.
RNA interference is a powerful technique for silencing specific genes and studying their functions. Oligonucleotides are crucial for the design of small interfering RNAs (siRNAs) and microRNAs (miRNAs). Our services help researchers develop optimized oligonucleotides for RNAi studies:
By using our oligonucleotide services, you can achieve highly specific and effective gene silencing for various functional genomics studies.
Oligonucleotides are at the heart of numerous diagnostic technologies and biosensor applications. Our oligonucleotide services are critical for the development of sensitive and specific diagnostic assays:
Our high-quality, customized oligonucleotides ensure that diagnostic assays are both reliable and sensitive, with applications in clinical diagnostics, food safety, environmental monitoring, and more.
Oligonucleotides are vital for preparing samples for NGS, one of the most powerful technologies for genomics. Our oligonucleotide services support NGS workflows by providing:
Our oligonucleotides are designed to maximize sequencing efficiency and accuracy, ensuring high-quality NGS results for diverse applications in genomics.
Understanding gene expression is crucial for studying cellular function, disease mechanisms, and therapeutic targets. Our oligonucleotide services enable accurate and sensitive gene expression studies:
By using our oligonucleotide services, researchers can gain precise insights into gene expression regulation, enhancing their understanding of biological processes and disease mechanisms.
Oligonucleotides have emerged as key components in the development of novel therapeutics, particularly for genetic diseases. Our services support oligonucleotide-based therapeutic research:
By providing high-quality, customizable oligonucleotide solutions, we support the development of cutting-edge gene therapies, vaccines, and other nucleic acid-based treatments.
Yes. We routinely synthesize oligos with up to 3–5 modifications per strand, including PS backbones, 2'-OMe sugars, and 5'/3' dyes or affinity tags. Whether you need complex chemical modifications or tailored labels, we ensure your oligos meet precise specifications.
Lyophilized or in buffered solution, in RNase/DNase-free conditions, with ice packs or dry ice depending on sequence sensitivity. Our shipping options are customizable to ensure your oligos arrive in optimal condition.
Yes. We can synthesize long oligonucleotides, up to 200 nt or more, with high fidelity. For longer sequences, we ensure proper annealing, purification, and quality control steps to ensure accuracy.
Yes. Each batch undergoes rigorous quality checks, including sequencing, mass spectrometry, or capillary electrophoresis, depending on the level of modification and the nature of the oligonucleotide.
Our minimum order quantity for standard oligonucleotides starts at 20 nmol, but we can accommodate smaller or larger orders depending on your needs. For specialized modifications, please consult our team for specific requirements.
We can synthesize nearly all sequences, but certain highly repetitive sequences or sequences with high GC content may present challenges. If you have a specific sequence in mind, we will review it and provide feedback on potential synthesis limitations.
Yes. Bulk pricing is available for large-scale orders. Our team will work with you to create a customized pricing structure based on your order volume, frequency, and specific requirements.
BOC Sciences provides a full spectrum of high-purity raw materials essential for precision oligonucleotide synthesis. These reagents are quality-assured, stringently tested, and optimized for both DNA and RNA chemistries.
Cap analogs are synthetic structures that mimic the 5' cap of eukaryotic mRNAs, critical for enhancing translation efficiency and stability in synthetic RNAs. We supply m⁷G(5')ppp(5')G and advanced ARCA analogs for capped RNA synthesis in in vitro transcription systems.
Controlled Pore Glass (CPG) is the solid-phase support used for oligonucleotide chain assembly. We offer standard and modified CPGs, including universal linkers and 3' modifications, with pore sizes tailored for oligos of varying lengths.
Nucleosides are the foundational units comprising a nitrogenous base and sugar, required for the synthesis of nucleotides and phosphoramidites. We provide a diverse set of protected and unprotected nucleosides for DNA, RNA, and modified oligo synthesis.
Nucleotides serve as the activated building blocks for enzymatic reactions like PCR, transcription, and ligation. Our inventory includes natural dNTPs/NTPs, as well as modified nucleotides (e.g., 5-methyl-dCTP, pseudouridine triphosphate) for specialized applications.
Phosphoramidites are the core reagents in automated solid-phase synthesis of oligonucleotides. We supply a wide range of DNA, RNA, and modified phosphoramidites, ensuring high coupling efficiency and compatibility with custom chemical modifications.
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