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LNA Gapmer oligonucleotides dramatically increase the specificity and sensitivity of the detection of non-coding RNA and other small RNA molecules. In addition to RNA, these powerful tools are ideal for the detection of low-abundance, short, or highly similar targets in many other research applications. BOC Sciences offers LNA gapmer oligonucleotide design and synthesis services for efficient applications in biology and medicine.
LNA Gapmer is a Gapmer oligonucleotide based on Locked Nucleic Acid (LNA) technology. LNA is a structurally specific nucleotide modifier that enhances the stability, affinity, and selectivity of the gapmer oligonucleotide by introducing an LNA modification into the nucleic acid chain. LNA has a locked loop structure that allows it to bind more tightly to the target sequence and improves base-pairing affinity. LNA Gapmer is usually designed using the Gapmer strategy, which involves the introduction of an antisense complementary region (gap) in the oligonucleotide sequence, which binds to a specific region of the target RNA and activates the activity of the RNase H enzyme, thereby cutting the target RNA.
LNA modification enhances the binding ability of the Gapmer oligonucleotide to the target sequence, increasing affinity and specificity.
LNA modification increases the stability of Gapmer oligonucleotide, resulting in a longer intracellular half-life.
LNA Gapmer enables selective targeting of target RNAs by designing specific antisense complementary regions and sequences.
LNA Gapmer can be used for gene silencing research and therapeutics to inhibit the expression of target genes by targeting specific RNA sequences.
BOC Sciences can help you design the LNA oligonucleotide that best suits your application and target. Your oligonucleotide will be designed for optimal LNA content and localization for best specificity and minimal secondary structure and self-complementarity. The high affinity of LNA Gapmer oligonucleotides for their complementary sequences significantly improves specificity and sensitivity compared to conventional DNA or RNA oligonucleotides. When designing LNA oligonucleotides, the incorporation of LNA into the sequence strongly affects the properties of the oligonucleotide, so it is necessary to find the correct design for the customer's experimental purposes.
Since LNA binds tightly to other LNA residues. Therefore, self-complementarity and cross-hybridization with other LNA-containing oligonucleotides need to be avoided.
Avoid stretches of more than 4 LNA bases unless designing very short (9-10 nucleotide) oligonucleotides.
Avoid stretches of 3 or more G or C.
The synthesis, purification and characterization of LNA Gapmer oligonucleotide can be found in the synthesis of gapmer oligonucleotide. Of course, it should be noted that in practice, some optimization and adjustment may be needed according to the specific design and target sequence.
Ways to optimize LNA Gapmer oligonucleotide include:
BOC Sciences is committed to providing high-quality LNA Gapmer oligonucleotide synthesis products. We utilize advanced synthesis techniques and quality control strategies to ensure the purity, consistency and stability of our products. If you are interested in our services, please contact us.
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