MicroRNA (miRNA) antagomirs have emerged as powerful tools for selectively silencing endogenous miRNAs, enabling functional studies of gene regulation and offering translational potential in therapeutic discovery. Unlike generic inhibitors, chemically modified and sequence-optimized antagomirs deliver superior stability, bioavailability, and target specificity. For researchers investigating complex disease mechanisms, gene expression pathways, or novel molecular targets, access to reliable custom synthesis of miRNA antagomirs is critical. BOC Sciences provides state-of-the-art custom miRNA antagomir synthesis services, designed to meet the stringent requirements of modern research and development.
Researchers working with miRNA inhibitors often face challenges that compromise both efficiency and reliability. BOC Sciences' synthesis solutions are engineered to address these issues directly:
Standard oligonucleotides may trigger unintended hybridization events, leading to misleading results. Our synthesis protocols leverage optimized backbone chemistries and proprietary modifications to enhance target fidelity.
miRNA antagomirs without proper stabilization degrade rapidly in biological systems. Our chemical modifications (such as phosphorothioate linkages and 2'-O-methyl modifications) extend half-life significantly, ensuring consistent gene silencing effects.
Poor transfection and intracellular delivery remain common bottlenecks. Through tailored conjugations and backbone designs, our custom antagomirs achieve superior uptake and sustained activity within cells.
Small variations in synthesis quality can lead to inconsistencies. We maintain strict process control and analytical validation to provide antagomirs with reliable batch-to-batch consistency.
By directly addressing these pain points, our services empower researchers to accelerate discovery while avoiding the pitfalls of suboptimal oligonucleotide design.
BOC Sciences offers a comprehensive and highly specialized portfolio of miRNA antagomir synthesis services, designed to empower researchers with precise, reliable, and functionally validated inhibitors of microRNA activity. Our approach goes far beyond generic oligonucleotide synthesis. By focusing on the unique biochemical, structural, and biological requirements of antagomirs, we provide researchers with tools that consistently deliver high specificity, stability, and reproducibility in complex experimental systems. Unlike standard antisense oligos, antagomirs require careful optimization of chemistry, conjugation, and delivery design to achieve meaningful inhibition of endogenous miRNAs. BOC Sciences supports clients at every stage—from sequence design and chemical modification to quality control and formulation—ensuring that each product is tailored to experimental goals.
| Standard miRNA Antagomirs | Description | Price |
| miRNA Antagomirs | Fully customized inhibitors designed for selective suppression of target miRNAs, incorporating optimized backbone chemistries to ensure nuclease resistance and reliable silencing. | Inquiry |
| miRNA Antagomir Negative Control | Scrambled or mismatch sequences with the same backbone modifications as the active antagomirs, essential for confirming specificity and ruling out non-specific effects. | Inquiry |
| miR-Down Antagomirs | Engineered for stronger and more persistent suppression of miRNAs, ideal for applications requiring robust and sustained knockdown across cell-based and preclinical studies. | Inquiry |
The backbone and sugar modifications of miRNA antagomirs determine their stability, affinity, and immunogenicity. At BOC Sciences, we provide a broad suite of chemically modified miRNA antagomirs tailored to overcome the limitations of unmodified oligonucleotides.
| Chemically Modified Antagomirs | Description | Price |
| Phosphorothioate (PS)-Modified miRNA Antagomirs | The substitution of non-bridging oxygen with sulfur in the phosphate backbone significantly enhances nuclease resistance, prolonging the functional half-life of the antagomir in biological systems. This modification is especially critical for in vivo models, where unprotected oligos degrade within minutes. | Inquiry |
| 2′-O-Methyl (2′-OMe) miRNA Antagomirs | Widely recognized for balancing stability and biological compatibility, 2′-OMe modifications increase binding affinity while reducing immune stimulation. These antagomirs are well-suited for sensitive experimental systems, such as primary cells or immunologically active tissues. | Inquiry |
| 2′-O-Methoxyethyl (2′-MOE) miRNA Antagomirs | Offering stronger hybridization and enhanced pharmacokinetic properties, 2′-MOE-modified antagomirs support applications where prolonged systemic circulation is required. Their improved biochemical properties help researchers maintain consistent silencing across longer timelines. | Inquiry |
| Locked Nucleic Acid (LNA)-Modified miRNA Antagomirs | LNA substitutions "lock" the ribose conformation, resulting in superior hybridization affinity and stability. This design allows potent inhibition even at low concentrations, making LNA-modified miRNA antagomirs particularly effective in challenging models where delivery efficiency is limited. | Inquiry |
A major barrier in miRNA inhibition research is intracellular delivery. BOC Sciences addresses this challenge by providing conjugated miRNA antagomirs, designed to improve uptake, tissue distribution, and experimental outcomes.
| Conjugated miRNA Antagomirs | Description | Price |
| Cholesterol-Conjugated miRNA Antagomirs | The addition of cholesterol enhances lipophilic interactions, promoting passive membrane crossing and efficient accumulation in hepatocytes and other lipid-rich tissues. This strategy is commonly used for liver-targeting studies, one of the most active areas of miRNA biology. | Inquiry |
| PEGylated miRNA Antagomirs | Polyethylene glycol (PEG) conjugation extends circulation time and reduces rapid clearance, allowing antagomirs to reach more diverse tissue targets. PEGylated variants are particularly beneficial for systemic studies where broad biodistribution is required. | Inquiry |
| Fluorescently Labeled miRNA Antagomirs | Incorporating fluorophores such as FAM, Cy3, or Cy5 allows real-time visualization of cellular uptake, subcellular localization, and distribution kinetics. These antagomirs are indispensable for delivery optimization and mechanism-of-action studies. | Inquiry |
| Biotin-Tagged miRNA Antagomirs | Biotinylated antagomirs enable affinity purification experiments, allowing researchers to capture miRNA–protein complexes or track binding partners. This functionality expands the role of antagomirs beyond inhibition into mechanistic exploration of RNA–protein interactions. | Inquiry |
The biological diversity of miRNAs demands flexibility in synthesis and customization. BOC Sciences provides target-specific miRNA antagomir services that adapt to various experimental contexts:
| Target-Specific Custom miRNA Antagomirs | Description | Price |
| Single miRNA Antagomirs | Focused inhibitors targeting one specific miRNA, suitable for functional validation studies, hypothesis-driven experiments, and mechanistic dissection of regulatory pathways. | Inquiry |
| Multi-miRNA Antagomir Panels | In many diseases, multiple miRNAs act synergistically. Our ability to synthesize panels of antagomirs in parallel allows researchers to probe entire miRNA families or regulatory clusters, providing broader insight into complex networks. | Inquiry |
| Species-Specific miRNA Antagomirs | miRNA sequences often vary across species. To ensure compatibility, BOC Sciences designs species-specific inhibitors tailored for human, mouse, rat, zebrafish, or other models. This ensures functional relevance and avoids misinterpretation due to sequence mismatches. | Inquiry |
Every project begins with a detailed assessment of the intended miRNA target. Our scientific team evaluates sequence conservation, potential secondary structures, and homologous regions to minimize off-target effects. This initial consultation ensures that the antagomir design is not only theoretiacally sound but also practically aligned with the researcher's goals.
Instead of relying on standardized templates, we employ advanced bioinformatics tools and proprietary design algorithms to create highly tailored antagomir structures. Key considerations include thermodynamic stability, mismatch tolerance, and hybridization kinetics. At this stage, clients receive comprehensive design proposals, allowing for direct feedback and refinements before synthesis begins.
Our synthesis process uses state-of-the-art automated platforms with optimized coupling chemistries. Each nucleotide incorporation step is carefully monitored to reduce truncations and side reactions. This precision ensures uniformity, even for sequences with difficult motifs or complex modifications.
A core strength of our service lies in customization. Whether incorporating phosphorothioate backbones for nuclease resistance, locked nucleic acids for enhanced affinity, or cholesterol conjugation for improved uptake, every modification is introduced with strict process control. This enables us to deliver antagomirs that are both durable and highly functional in biological systems.
To guarantee structural integrity and functional reliability, synthesized antagomirs undergo multiple purification steps, including HPLC and PAGE, followed by rigorous analytical validation through LC-MS and UV spectrometry. This ensures that clients receive oligonucleotides with the highest possible purity, free from truncated species or synthesis by-products.
Beyond the product itself, we provide full analytical documentation, including certificates of analysis and batch-specific QC reports. These data packages enable seamless integration into research workflows and support regulatory submissions in preclinical development pipelines.
Final products are packaged according to client specifications, whether as lyophilized vials or ready-to-use solutions. Stability-tested shipping conditions safeguard product integrity during transit. After delivery, our dedicated technical team remains available to address experimental integration questions, ensuring smooth adoption into downstream applications.
Selecting the right partner for custom miRNA antagomir synthesis is critical for achieving reliable, reproducible, and high-impact results. BOC Sciences is trusted globally for its expertise in oligonucleotide chemistry and its commitment to scientific excellence.
Our team brings decades of combined experience in synthetic oligonucleotides, ensuring every antagomir is designed with precision and produced to the highest scientific standards.
We offer a wide array of chemical modifications, conjugations, and backbone designs, allowing researchers to fine-tune antagomirs for stability, specificity, and functional performance in diverse experimental systems.
Every product undergoes rigorous multi-step purification and is validated by advanced analytical techniques such as HPLC and LC-MS, ensuring identity, integrity, and exceptional purity.
Whether you need small research-scale quantities for discovery projects or larger batches for advanced studies, we provide scalable synthesis with consistent quality across all production sizes.
BOC Sciences has established collaborations with leading research institutions and biotechnology companies worldwide, delivering antagomir solutions that accelerate discovery and innovation.
Beyond synthesis, our scientific team provides personalized consultation to help clients with sequence design, modification strategies, and integration into downstream workflows.
By combining technical depth, strict quality control, and tailored solutions, BOC Sciences empowers researchers to overcome the limitations of conventional miRNA inhibitors and confidently advance their projects.
Custom miRNA antagomirs synthesized by BOC Sciences enable researchers to dissect complex regulatory networks, validate novel therapeutic targets, and accelerate innovation across molecular biology and biotechnology. Their versatility and robustness make them indispensable tools in both fundamental research and advanced preclinical studies.
By selectively silencing endogenous miRNAs, antagomirs reveal how individual miRNAs control gene expression networks. For example, inhibition of miR-21 has been used to uncover its regulatory role in tumor suppressor signaling. Our high-purity antagomirs minimize off-target noise, ensuring that pathway dissection reflects true biological effects rather than artifacts of poor oligo quality.
Antagomirs are powerful tools for validating whether a miRNA contributes to disease phenotypes. In cardiovascular research, miR-92a inhibition has been shown to promote angiogenesis, supporting its role as a therapeutic target. BOC Sciences' custom synthesis allows researchers to design antagomirs against disease-relevant miRNAs with robust nuclease resistance, enabling reliable in vitro and in vivo validation studies.
Many diseases, including cancers and metabolic disorders, exhibit miRNA dysregulation. Custom antagomirs help determine whether candidate miRNAs act as drivers or mere bystanders. For instance, blocking miR-155 can clarify its contribution to inflammatory responses. Our synthesis service provides labeled or conjugated antagomirs, facilitating mechanistic studies and biomarker validation.
Pharmaceutical pipelines increasingly evaluate miRNA modulation as a therapeutic strategy. Custom antagomirs synthesized at BOC Sciences allow drug discovery teams to rapidly generate preclinical proof-of-concept data. With scalable synthesis and rigorous analytical validation, our products fit seamlessly into early-stage research pipelines without concerns about batch-to-batch variability.
The performance of lipid nanoparticles, polymer carriers, or other delivery vehicles often depends on the oligonucleotide cargo. Custom antagomirs serve as test molecules for assessing delivery efficiency, biodistribution, and release kinetics. With options for conjugation (e.g., cholesterol, PEG, fluorophores), BOC Sciences enables researchers to tailor antagomirs for side-by-side evaluation of delivery platforms.
Antagomirs can simulate therapeutic interventions at the preclinical stage. For example, blocking miRNAs associated with chemoresistance allows scientists to evaluate whether tumor cells regain sensitivity to conventional drugs. High-quality custom antagomirs ensure that observed effects are reproducible and directly linked to the targeted miRNA.
miRNA antagomir is a synthetic small molecule RNA that can bind to miRNA to form a complex, thus blocking the function of miRNA. Similar to miRNA, miRNA antagomir is also a short-stranded RNA, usually 20-25 nucleotides in length. miRNA antagomir has a similar structure to miRNA, but its sequence is complementary to miRNA so that it can effectively bind and block the function of miRNA.
The mechanism of miRNA antagomir is mainly to block the function of miRNAs by competitively binding miRNAs. miRNA antagomir binds to miRNAs and can block the binding of miRNAs to target genes, thus affecting the regulatory effect of miRNAs. In addition, miRNA antagomir can promote the degradation of miRNAs and inhibit miRNA biosynthesis.
BOC Sciences offers a full range of backbone and sugar modifications—such as phosphorothioate, 2′-O-methyl, LNA, and MOE—as well as custom conjugations like cholesterol or fluorescent dyes. These modifications enhance nuclease resistance, hybridization affinity, and intracellular delivery efficiency.
Custom miRNA antagomirs provide superior specificity, extended stability, and lower off-target interactions compared to generic inhibitors. With optimized modifications, they ensure consistent results in functional genomics and preclinical research.
Yes. We provide scalable oligonucleotide synthesis from small milligram quantities for early experiments up to gram-scale production suitable for advanced preclinical research. Our scalability ensures consistency across every batch.
Yes, we offer a wide range of labeling options including fluorophores, biotin, and quencher molecules. These enable visualization, live-cell tracking, and capture of RNA-protein complexes for advanced studies.
Absolutely. Our experts can retrieve validated sequences from miRNA databases and design custom antagomirs tailored to your experimental goals, ensuring optimal specificity and activity.
Empower your research with the confidence of working with a trusted global partner in oligonucleotide synthesis.
Get A Quote
Global Supplier of High-Quality RNA / DNA Products & Services.
