Custom miRNA Antagomir Synthesis

miRNA antagomir is a small single-stranded RNA designed according to the mature microRNA sequence, which is specially labeled and chemically modified. BOC Sciences provides miRNA antagomir synthesis services to accelerate miRNA research progress.

Introduction to miRNA Antagomir

• What is miRNA

microRNA (miRNA) is a small, siRNA-like molecule encoded by the higher eukaryotic genome, highly conserved in species evolution. miRNAs are found in plants, animals, and fungi and are expressed only at specific tissues as well as developmental stages. The specificity and developmental stage of miRNA determine the function of tissues and cells, and miRNA has demonstrated multiple roles in regulating cell growth and developmental processes.

• miRNA Biogenesis Pathway

miRNA is transcribed by RNA polymerase II into a primary transcription product, pri-miRNA, containing a 5' end cap and a polyA tail. pri-miRNA is processed in the nucleus by a complex composed of the RNase III enzyme Drosha and the double-stranded RNA-binding protein Pasha/DGCR8 to obtain a pre-miRNA of 70 nucleotides in length that is folded into a stem-loop. Then, pre-miRNA is exported into the cytoplasm via the Exp5 transporter protein. Once in the cytoplasm, pre-miRNA is processed by the RNAse III enzyme Dicer to afford the double-stranded RNA of approximately 22 nucleotides in length. Dicer also initiates the formation of RNA-induced silencing complex (RISC), which mediates gene silencing through miRNA expression and RNA interference.

Fig. 1 miRNA biogenesis pathway (Hajarnis S, 2015)

• Definition of miRNA Antagomir

miRNA antagomir is a synthetic small molecule RNA that can bind to miRNA to form a complex, thus blocking the function of miRNA. Similar to miRNA, miRNA antagomir is also a short-stranded RNA, usually 20-25 nucleotides in length. miRNA antagomir has a similar structure to miRNA, but its sequence is complementary to miRNA so that it can effectively bind and block the function of miRNA.

• Mechanism of miRNA Antagomir

The mechanism of miRNA antagomir is mainly to block the function of miRNAs by competitively binding miRNAs. miRNA antagomir binds to miRNAs and can block the binding of miRNAs to target genes, thus affecting the regulatory effect of miRNAs. In addition, miRNA antagomir can promote the degradation of miRNAs and inhibit miRNA biosynthesis.

Our miRNA Antagomir Synthesis Service Content

• Introduction to miRNA Antagomir Service

1. Synthesis of miRNA antagomir - Synthesize miRNA antagomir with high purity and efficiency according to the miRNA sequence provided by the customer to inhibit the function of miRNA.
2. Purify miRNA antagomir - Purify miRNA antagomir by high performance liquid chromatography (HPLC) or capillary electrophoresis (CE) and other techniques to ensure its purity meets the requirements.
3. Validate the effect of miRNA antagomir - Validate the effect of miRNA antagomir by cell experiment or animal experiment, etc. to ensure its high efficiency and specificity.
4. Provide relevant technical support - BOC Sciences' expert team provides relevant technical support to help customers solve problems encountered in experiments, and provide relevant advice and guidance.
5. Provide customized services - According to the needs of customers, we provide personalized miRNA antagomir synthesis services to meet the needs of different experiments.

• Modifications for miRNA Antagomir

1. 3'-end cholesterol modification
2. Four thio-backbone modifications at the 3' end
3. Two thio-backbone modifications at the 5' end
4. Whole chain 2' methylation modification

• miRNA Antagomir Ordering Information

Features of BOC Sciences' miRNA Antagomir

• miRNA antagomir possessed higher stability and inhibitory effects in vitro and in vivo than conventional inhibitors.
• miRNA antagomir can enrich target cells by overcoming cell membrane and tissue barriers in vivo.