TAMRA-Phos-CPG, 6-Carboxy Single Isomer (5'-DMT-mdC(TEG-TAMRA)-Phos-CPG); 1000 Å
TAMRA-Phos-CPG, 6-Carboxy Single Isomer is used for 3-TAMRA (rhodamine) labelling of an oligonucleotide, with a TEG spacer.
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Absorption Maximum (Lambda Max)
555
Synthesis Conditions
Synthesis can be performed on these supports according to standard procedures with deprotection and cleavage modifications noted. The phosphate linkage is susceptible to degradation if the Tamra CPG support is left at room temperature (25°C) over a 24 hour period. The subsequent cleavage may affect the overall yield.
Cleavage Conditions
Use t-butylamine/water (1:3) for 45 minutes at 25 °C. The use of ammonia or methylamine must be avoided, as it will cause degradation of the TAMRA moiety.
Deprotection Conditions
When using fast deprotecting amidites (eg. C-Ac; G-DMF; G-PAC) use t-butylamine/Water (1:3) for 6 hours at 60°C. When using standard amidites (eg. C-Bz; G-iBu) use t-butylamine/Water (1:3) overnight (12-15 hours) at 60°C.
Chemical Structure:
