Large Scale RNA Oligonucleotide Synthesis

Due to the great potential of RNA applications in biomedical fields, including mRNA vaccine production, gene therapy and antiviral drug development, large-scale production of RNA is increasingly required. Here, BOC Sciences is committed to providing large-scale RNA oligonucleotide production, which facilitates our customers to obtain large quantities of RNA within a short period of time, and greatly advances the research process of RNA drugs.

Biomedical Applications of RNA

Increasing research into RNA function and its critical role in various diseases has led to the development and application of various RNA drugs. BOC Sciences offers large-scale RNA oligonucleotide synthesis services to meet the growing demand for RNA in the biopharmaceutical global market and to better expand the scope of RNA drug applications.

• Antisense Oligonucleotides (ASOs): ASOs are short single-stranded(ss) oligonucleotides (12-24 nucleotides (nt)) that complement specific RNAs through Watson-Crick base pairing. ASOs can reduce, restore, or modify protein expression by regulating RNA.
• RNA Interference (RNAi): RNAi is an endogenous cellular process that induces double-stranded (ds) RNA to trigger the degradation of specific RNA targets, thus providing an intrinsic defense mechanism against invading viruses and transposable elements.
• CRISPR-based Genome Editing: CRISPR systems rely on engineered guide RNA (gRNA) and RNA-guided Cas nuclease. gRNA binds to Cas to form the Cas-gRNA ribonucleoprotein complex, which recognizes prototypical spacer-adjacent motif (PAM) elements and 20-nucleotide sequences in the target sequence. Cas nuclease then cleaves the dsDNA or ssRNA at specific sites for efficient genome editing.
• Aptamer: Aptamers are single-stranded oligonucleotides with a well-defined three-dimensional structure that specifically bind and inhibit proteins. They are also known as chemical antibodies due to their synthetic origin and similar mode of action to antibodies.
• mRNAs and mRNA Vaccine: Mechanistically, the injected mRNA vaccine is delivered into the cytoplasm of a host cell, usually an antigen-presenting cell (APC), and translated into the target antigen. Subsequently, the major histocompatibility complex (MHC) presents the expressed antigen to the surface of the APC to activate B cell/antibody-mediated humoral immunity and CD4+ T/CD8+ cytotoxic T cell-mediated immunity.

Figure 1. Examples of RNA-based therapeutics. (Zhu, Y, R.;et al. 2022)

Large Scale RNA Oligonucleotide Synthesis Services from BOC Sciences

BOC Sciences is committed to providing large-scale RNA oligonucleotide synthesis services to customers worldwide, supporting the demand for large quantities of RNA Oligonucleotides in the development of oligonucleotide drugs, siRNAs, gene editing technologies, aptamer drugs and mRNA vaccines.

RNA Oligonucleotide Sequence Design (or Customer Supplied Templates directly): BOC Sciences' experienced specialists design oligonucleotide sequences based on the client's specific research needs or gene targets.

Synthesis Protocol Development: Performed by a team of experts, BOC Sciences assists you in determining the number and length of oligonucleotides to be synthesized, selecting appropriate synthesis methods and purification strategies, etc.

High-throughput Synthesis of RNA Oligonucleotides: BOC Sciences adheres to GMP grade RNA oligonucleotide synthesis while ensuring large scale and low cost. We are confident that our large-scale RNA oligonucleotide products are competitively priced.

Figure 2. BOC Sciences' large-scale RNA oligonucleotide synthesis service flow.

Advantages of Large Scale RNA Oligo Synthesis Service

Efficient Manufacturing: BOC Sciences' large-scale synthesis technology enables the synthesis of large quantities of RNA oligonucleotides in a short period of time, which is particularly suitable for high-throughput experimental and preclinical research needs.

Highly Customizable: BOC Sciences' large-scale synthesis solutions are fully customizable for mRNA vaccine development, gene regulation and genomics research.

High Purity and Uniformity: BOC Sciences' large-scale synthesis technology ensures high purity and quality uniformity of RNA oligonucleotides while reducing batch-to-batch variability.

Competitive Pricing: BOC Sciences has been insisting on continuously updating and optimizing the synthesis technology and process to reduce the cost of large-scale RNA oligonucleotide synthesis as much as possible, in order to provide customers with the most competitive pricing.

For more detail about our large scale RNA oligonucleotide synthesis services, please feel free to contact us.