Custom miRNA Agomir Synthesis

miRNA agomir is a specially labeled and chemically modified double-stranded small RNA. BOC Sciences provides miRNA agomir synthesis services to accelerate miRNA research progress.

What is miRNA Agomir

• Introduction to miRNA

miRNA, also known as microRNA, is an RNA molecule of 21-23 nt in length that widely exists in eukaryotes. miRNA inhibits mRNA transcription by binding to mRNA. Therefore, it plays an important role in regulating gene expression, cell cycle, and developmental events of organisms.

Fig. 1 miRNA biogenesis and functions (Alsharafi WA, 2015)

• Definition of miRNA Agomir

miRNA agomir is a specially labeled and chemically modified double-stranded small RNA (miRNA agonist) that regulates the biological functions of target genes by mimicking endogenous miRNA into the miRiSC complex.

• Basic Principle of miRNA Agomir

miRNA agomir is a synthetic miRNA molecule that mimics natural miRNA molecules, binding to mRNA of target genes and inhibiting their translation and expression. Compared to traditional siRNAs, miRNA agomir has a broader targeting and longer-lasting effect. miRNA agomir synthesis requires strict quality control and purification to ensure its safety and efficacy.

• Application Scenarios for miRNA Agomir

miRNA agomir has a wide range of application scenarios and can be used to treat a variety of diseases, such as cancer, cardiovascular diseases, diabetes, etc. As an example, miR-34a agomir can inhibit the proliferation and metastasis of tumor cells, thus enabling tumor treatment. In addition, miRNA agomir can be used in gene editing, stem cell therapy, and other fields.

Our miRNA Agomir Synthesis Service Content

1. Synthesize miRNA agomir - Synthesize the corresponding miRNA agomir according to the miRNA sequence information provided by customers.
2. Purify miRNA agomir - Purify the synthesized miRNA agomir by ion exchange chromatography, reverse phase high performance liquid chromatography and other techniques to improve the product purity.
3. Identify miRNA agomir - Use techniques such as mass spectrometry and nuclear magnetic resonance to identify the synthesized miRNA agomir to ensure the quality and purity of the product.
4. Packaging miRNA agomir - The purified miRNA agomir will be packaged according to customer requirements, and different sizes of packaging can be provided, such as milligram level, microgram level, etc.
5. Provide warranty service - Provide warranty service for miRNA agomir to ensure the quality and stability of the product.
6. Provide technical support - Provide the usage and technical support of miRNA agomir to ensure customers can use the product smoothly.

Advantages of BOC Sciences' miRNA Agomir Service

• Covers human, mouse, rat miRNA
• miRNA agomir possessed a higher affinity with cell membrane than common miRNA mimics and miRNA inhibitors
• Higher stability and inhibitory
• Enriched in target cells to achieve efficient specificity and stable interference
• Suppression lasts over an extended period(at least one week) and interference effect lasts up to 5-6 weeks