2'-O-Methyl-G(iBu)-Suc-CPG; 500 Å (RNA)

2'-O-Methyl-G(iBu)-Suc-CPG; 500 Å is a reagent used in the solid-phase synthesis of RNA oligonucleotides, specifically for incorporating 2'-O-methylguanosine residues. It includes an isobutyryl-protected guanine base and a succinyl linker attached to controlled pore glass (CPG) with a 500 Å pore size. The 2'-O-methyl modification enhances the RNA's stability and resistance to enzymatic degradation, making this reagent ideal for synthesizing RNA sequences with improved durability and precise guanine incorporation.

* Please kindly note that our products are not to be used for therapeutic purposes and cannot be sold to patients.

Catalog

BRP-02244

Synonyms

2'-OMe-G(iBu) CPG; 2'-OMe-rG(iBu) CPG; 2'-OMe-G-RNA-CPG; 5'-O-(4,4'-Dimethoxytrityl)-N2-isobutyryl-2'-O-methylguanosine-3'-Suc-CPG 500 Å; N2-Isobutyryl-2'-O-methyl-5'-O-DMT-guanosine-3'-Suc-CPG 500 Å; N2-Isobutyryl-2'-O-methyl-5'-O-dimethoxytritylguanosine-3'-Suc-CPG 500 Å

Appearance

White to off-white powder

Storage

Store at -20 °C

Symbol

2'-OMe-G-RNA-CPG

Shipping

Room temperature.

Cleavage Conditions

Use concentrated ammonia for 90 min at 25°C or 30 min at 60°C, or 1:1 ammonia:methylamine (AMA) for 25 min at 25°C when using fast deprotecting amidites.

Deprotection Conditions

When using fast deprotection amidites (such as C-Ac; G-DMF), use concentrated ammonia at 60°C for 1 hour or AMA for 30 minutes. When using standard amidites (such as C-Bz; G-iBu), please use concentrated ammonia at 60°C for 5 hours. Compatible with the deprotection chemicals used by 2' Fluoro; 2' modified phosphoramidite and TBDMS protected reagents.

Chemical Structure:

Reference Reading

1. Antimicrobial Activity of Ibuprofen against Cystic Fibrosis-Associated Gram-Negative Pathogens

Parth N Shah, Kimberly R Marshall-Batty, Justin A Smolen, Jasur A Tagaev, Qingquan Chen, Christopher A Rodesney, Henry H Le, Vernita D Gordon, David E Greenberg, Carolyn L Cannon. Antimicrob Agents Chemother. 2018 Feb 23;62(3):e01574-17. doi: 10.1128/AAC.01574-17.

Clinical trials have demonstrated the benefits of ibuprofen therapy in cystic fibrosis (CF) patients, an effect that is currently attributed to ibuprofen's anti-inflammatory properties. Yet, a few previous reports demonstrated an antimicrobial activity of ibuprofen as well, although none investigated its direct effects on the pathogens found in the CF lung, which is the focus of this work. Determination of ibuprofen's in vitro antimicrobial activity against Pseudomonas aeruginosa and Burkholderia species strains through measurements of the endpoint number of CFU and growth kinetics showed that ibuprofen reduced the growth rate and bacterial burden of the tested strains in a dose-dependent fashion. In an in vitroPseudomonas biofilm model, a reduction in the rate of biomass accumulation over 8 h of growth with ibuprofen treatment was observed. Next, an acute Pseudomonas pneumonia model was used to test this antimicrobial activity after the oral delivery of ibuprofen. Following intranasal inoculation, ibuprofen-treated mice exhibited lower CFU counts and improved survival compared with the control animals. Preliminary biodistribution studies performed after the delivery of ibuprofen to mice by aerosol demonstrated a rapid accumulation of ibuprofen in serum and minimum retention in lung tissue and bronchoalveolar lavage fluid. Therefore, ibuprofen-encapsulated polymeric nanoparticles (Ibu-NPs) were formulated to improve the pharmacokinetic profile. Ibu-NPs formulated for aerosol delivery inhibited the growth of P. aeruginosa in vitro and may provide a convenient dosing method. These results provide an additional explanation for the previously observed therapeutic effects of ibuprofen in CF patients and further strengthen the argument for its use by these patients.

2. Ibuprofen and COVID-19 disease: separating the myths from facts

Frideriki Poutoglidou, Athanasios Saitis, Dimitrios Kouvelas. Expert Rev Respir Med. 2021 Aug;15(8):979-983. doi: 10.1080/17476348.2021.1951239.

Introduction: The Coronavirus disease 2019 (COVID-19) poses novel challenges in the healthcare systems around the world. Concern about the role of Non-Steroidal Anti-Inflammatory Drugs (NSAIDs) and, in particular, ibuprofen has led to significant speculation.Areas covered: A literature search was conducted to evaluate ibuprofen's potential benefits and harms in the COVID-19 disease. Angiotensin-Converting Enzyme 2 (ACE-2) is crucial entry receptor for Severe Acute Respiratory Syndrome coronavirus-2 (SARS-CoV-2) in host cells. We found no scientific evidence linking ibuprofen use and an ACE-2 overexpression. Ibuprofen suppresses the production of various pro-inflammatory cytokines that are implicated in the 'cytokine storm' and subsequent ARDS in COVID-19 disease. Nevertheless, the exact role of ibuprofen in the immune response in COVID-19 disease is still unknown. There are no double-blind, placebo-controlled studies assessing the effect of ibuprofen on COVID-19 disease progression.Expert opinion: The studies that have been performed so far demonstrate no association between ibuprofen use and increased mortality rates or an increased risk for respiratory support. Accordingly, we recommend ibuprofen to be used for managing COVID-19 symptoms.

3. Gelatin-glucosamine hydrochloride/crosslinked-cyclodextrin metal-organic frameworks@IBU composite hydrogel long-term sustained drug delivery system for osteoarthritis treatment

Hui Yang, Yinchun Hu, Min Kang, Huixiu Ding, Yue Gong, Xiangfei Yin, Ruize Sun, Yuhong Qin, Yan Wei, Di Huang. Biomed Mater. 2022 Apr 11;17(3). doi: 10.1088/1748-605X/ac61fa.

Osteoarthritis (OA) is a disease of articular cartilage degradation and inflammation of the joint capsule. Combining anti-inflammatory therapy with nutritional supplement is an effective means for the treatment of OA. In this study, we prepared gelatin (Gel)-glucosamine hydrochloride (GH) mixed crosslinked-cyclodextrin metal-organic framework (G-GH/CL-CD-MOF) composite hydrogel. Polyethylene glycol diglycidyl ether was the crosslinking agent of GH and Gel to solve the problem of poor mechanical properties and water solubility at 37 °C. CL-CD-MOF was fabricated through a simple one-step chemical reaction to crosslink the hydrophilic hydroxyl groups in CD-MOF with diphenyl carbonate. Electron microscopy and x-ray diffraction analysis of CL-CD-MOF showed perfect porous morphology with a chaotic internal structure. CL-CD-MOF@IBU was prepared by immersing CL-CD-MOF in high-concentration ibuprofen (IBU) solution. CL-CD-MOF@IBU was uniformly dispersed in Gel and GH mixed solution to prepare G-GH/CL-CD-MOF@IBU composite hydrogel long-term sustained drug delivery system. The compression curve of G-GH/CL-CD-MOF composite hydrogel showed a non-linear elastic behavior. The cyclic loading-unloading compression showed that the shape of the G-GH/CL-CD-MOF composite hydrogel can be kept intact under 50% strain. On the day 14, the G-GH/CL-CD-MOF@IBU composite hydrogel was degraded by 87.1%, 61% of IBU was released. G-GH/CL-CD-MOF@IBU exhibited good biocompatibility during co-culture with MC3T3-E1 cells. Briefly, the certain mechanical properties, sustained drug release behavior, and good biocompatibility of G-GH/CL-CD-MOF@IBU composite hydrogel showed that it has potential application in OA treatment of long-term sustained nutritional supplement and anti-inflammatory synchronously.