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What is Gene Editing?
Gene editing is a technique that allows researchers to modify DNA or RNA sequences in a precise, targeted way. It involves the use of enzymes that can cut DNA or RNA at specific locations, allowing researchers to delete, add, or replace genetic material.
The CRISPR/Cas9 system, one of the most widely used gene editing tools, consists of an RNA-guided endonuclease and a guide RNA (gRNA) that guides Cas9 to the target DNA sequence. Upon binding to DNA, the Cas9-gRNA complex induces a double-strand break, which can be repaired by non-homologous end joining or homology-directed repair.
Cas9 Utilizes mRNA to Induce Double-stranded Breaks
The CRISPR/Cas9 system is based on the bacterial immune system, which uses RNA-guided nucleic acid endonucleases to target and destroy invading DNA or RNA. Cas9 proteins are nuclease enzymes that cleave DNA or RNA at a specific location guided by the system's RNA components.
One of the challenges of using the CRISPR/Cas9 system is delivering the Cas9 and gRNA components to the target cell. One solution is to use mRNA as a delivery vehicle, which can be easily synthesized and efficiently translated into proteins upon entry into the cell. mRNA delivery has been shown to be effective in a variety of cell types, including human cells, and has the added advantage of being transient, reducing the risk of off-target effects.
How does Gene Editing Work?
Gene editing can be used to introduce specific genetic changes in a wide range of organisms, from bacteria to plants and animals. To use gene editing, researchers first identify the target DNA or RNA sequence they want to modify and design a guide RNA that directs the Cas9 enzyme to the correct location.
Once the Cas9-gRNA complex binds to the target DNA or RNA, it induces a double-strand break, which can be repaired by two routes: NHEJ or HDR. NHEJ is an error-prone repair mechanism that introduces a small insertion or deletion (indel) at the break site, resulting in a loss-of-function or a shifted mutation. HDR, on the other hand, is a precise repair mechanism that uses a donor DNA template to introduce specific changes at the break site, such as the addition of a new gene or the correction of a disease-causing mutation.
Advantages of Gene Editing
Precision and Specificity - With gene editing, we can pinpoint and modify specific genes to avoid unintended effects on the rest of the genome.
Superior Efficiency - Another advantage of gene editing is its efficiency. the CRISPR/Cas9 system can efficiently induce changes in target genes, thereby reducing the time and cost required for genetic engineering. In addition, gene editing can be used to introduce genetic alterations into cells or organisms that are difficult to manipulate genetically using traditional methods.
New Therapeutic Modalities - Gene editing also has the potential to revolutionize the field of medicine by providing new treatments for genetic diseases. By correcting disease-causing mutations or introducing specific genes, gene editing has the potential to cure inherited diseases such as cystic fibrosis, sickle cell anemia and Huntington's disease.
For more DNA & RNA synthesis raw materials or research tools in gene editing, please feel free to contact us.
* Only for research. Not suitable for any diagnostic or therapeutic use.
