ssDNA Oligo Synthesis

What is ssDNA Oligo?

ssDNA oligos are short strands of DNA molecules composed of single stranded nucleotides. Single-stranded DNA (ssDNA) oligos are important tools in molecular biology research, diagnostics, gene therapy, and DNA nanotechnology. BOC Sciences adheres to strict quality control standards to ensure our products meet the highest standards of purity and quality. Our professionals can analyze the purity and identity of our products through professional analytical instruments.

BOC Sciences' ssDNA Oligo Synthesis Services

BOC Sciences offers a range of ssDNA oligonucleotide synthesis development services to meet the growing demand for these important research tools. Our services are designed to provide high quality customized solutions to meet the specific needs of our clients.

Solvent phase DNA synthesis is usually used to synthesize ssDNA oligonucleotides. However, as the length of the oligonucleotide increases with chemical methods, the yield will decrease. Therefore, synthesizing long, high quality DNA by chemical methods is not effective in current chemical research. BOC Sciences uses an MOSIC method that can produce ssDNA oligo with industry-leading low error rates.

• How to Prepare ssDNA Oligo?

The experimental procedure for synthesizing ssDNA oligonucleotides using the MOSIC method consists of several steps:

a) Amplification of cloning templates from a single bacterial colony.

b) Digestion of cleaved hairpins in the product, releasing a pool of oligonucleotides with precisely controlled relative stoichiometry.

c) Purified ssDNA oligonucleotides were analyzed using HPLC and MS to ensure high purity and identity.

d) Quality control to ensure ssDNA oligonucleotides meet or exceed customer specifications.

f) Custom ssDNA oligonucleotides to meet the specific needs of each project, including modification and labeling.

• Design of ssDNA Oligo

• Sequence Selection

Determine the desired target sequence, which may be a specific DNA sequence associated with a target gene, promoter, binding site, etc.

• Base Composition

Balance the base composition to avoid too many specific bases in the sequence, such as GC or AT. Considering the stability and hybridization performance under experimental conditions, the base composition can be adjusted according to the use and purpose of the oligonucleotide.

• Length Selection

Determine the desired length of the oligonucleotide based on experimental needs and design purpose. Shorter oligonucleotides are usually easier to synthesize, but the length may limit their specific experimental application.

• Specialized Structures or Modifications

Special structures or modifications, such as the introduction of specific sequence repeats, the introduction of chemical modification groups, or special structural elements, can be introduced into the design of an oligonucleotide, depending on the need.

BOC Sciences' Advantages of Synthesis of ssDNA Oligo

The MOSIC method offers several advantages over traditional solid-phase synthesis methods. These advantages include:

• Highly pure and stoichiometric ssDNA oligonucleotides.
• Long ssDNA oligonucleotides with precise control of relative stoichiometry.
• Scalability, allowing mass production of ssDNA oligonucleotides.
• Flexibility and customization to meet the specific needs of each project.
• State-of-the-art equipment and analytical techniques ensure high quality and consistent results.
• Timely and efficient delivery of final products to our customers.

BOC Sciences has leading ssDNA Oligo synthesis, purification and analysis platforms and professionals to provide high quality and reliable cGMP-grade and non-cGMP-grade oligonucleotide products. Contact us and our professionals will customize the best service for your project according to your needs.