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Custom miRNA Sponges Synthesis

MicroRNAs (miRNAs) play crucial roles in regulating various biological processes, from development to disease. Understanding their functions and mechanisms of action is essential for unraveling complex gene regulatory networks. One powerful tool for studying miRNA function is the use of miRNA sponges, synthetic RNA molecules designed to sequester specific miRNAs and inhibit their activity.

What are miRNA Sponges?

miRNA sponges, also known as competitive endogenous RNA (ceRNA) molecules, are synthetic transcripts containing multiple binding sites for a specific miRNA. These sponges function as competitive inhibitors by sequestering miRNAs and preventing them from binding to their target mRNAs, thus derepressing the expression of miRNA-regulated genes. By titrating specific miRNAs, miRNA sponges enable researchers to investigate the biological functions of miRNAs and their roles in various physiological and pathological processes.

Schematic diagram of the functions and roles of miRNA sponges.(A) In the absence of sponge treatment, the target mRNA (grey) of a specific miRNA seed family (red complex) is inhibited; (B) Upon introduction of sponge transgene, sponge mRNA (green) is expressed at high levels, sequestering miRNA complexes and rescuing the expression of endogenous targets.

miRNA sponges contain multiple high-affinity binding sites for miRNA. When miRNA sponges are highly expressed in cells, they can inhibit the activity of miRNA families that share the same seed sequence (2-7 nucleotides in the miRNA that determine the specificity of target gene recognition). miRNA sponges can be highly expressed in target cells through transient transfection. Transfection methods may include lipofection or electroporation, and different promoters can be selected, such as CMV, U6 promoters, and tissue-specific promoters. Different types of vectors, such as inducible and constitutive expression vectors, can also be chosen, along with different reporter genes to monitor different cellular phenotypes, such as GFP, luciferase, etc. To enhance infection efficiency, methods like lentivirus, adenovirus, or adeno-associated virus can be utilized.

BOC Sciences' Custom miRNA Sponges Service

BOC Sciences is proud to offer a custom miRNA sponge synthesis service, providing researchers with tailor-made miRNA sponge constructs to facilitate their miRNA studies. Our expert team of scientists utilizes state-of-the-art techniques and cutting-edge technology to design and synthesize high-quality miRNA sponge vectors tailored to the specific needs of our clients. There are various ways regarding the sequence design of miRNA sponges, including bulged sponges, direct antisense design, TuD (tough decoy) RNA design, etc. BOC can choose the most appropriate cloning strategy to construct MBS-containing sponges according to the needs, which can be used for the inhibition of one, two, or more than two miRNAs, respectively.

Types of miRNA Sponge from BOC Sciences

Notes for BOC Sciences' Custom miRNA Sponge Service

Applications of miRNA Sponges from BOC Sciences

Advantages of miRNA Sponges from BOC Sciences

If you have custom synthesis needs for more types of miRNA Sponges, please feel free to contact us.

Reference

  1. M, S, Ebert and P, A, Sharp. MicroRNA Sponges: Progress and Possibilities. RNA. 2010, 16(11): 2043–2050.
* Only for research. Not suitable for any diagnostic or therapeutic use.
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