ROX-CPG (5'-DMT-mdC(TEG-ROX)-Phos-CPG); 500 Å

ROX-CPG (5'-DMT-mdC(TEG-ROX)-Phos-CPG); 500 Å

ROX-CPG (5'-DMT-mdC(TEG-ROX)-Phos-CPG); 500 Å is a reagent used in the solid-phase synthesis of oligonucleotides that incorporates a 5(or 6)-carboxy-X-rhodamine (ROX) fluorescent dye. The reagent includes 5'-DMT (dimethoxytrityl) for protecting the 5' end, a methylated deoxycytidine (mdC) residue, and a triethylene glycol (TEG) spacer linked to the ROX dye. The phosphoramidite is attached to controlled pore glass (CPG) with a 500 Å pore size. This configuration allows for the incorporation of a fluorescent marker into oligonucleotides, which is commonly used in fluorescence-based assays and detection methods. The TEG spacer provides flexibility and reduces steric hindrance, improving the dye's interaction with other molecules or probes in assays.