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ROX-CPG (5'-DMT-mdC(TEG-ROX)-Phos-CPG); 500 Å
ROX-CPG (5'-DMT-mdC(TEG-ROX)-Phos-CPG); 500 Å is a reagent used in the solid-phase synthesis of oligonucleotides that incorporates a 5(or 6)-carboxy-X-rhodamine (ROX) fluorescent dye. The reagent includes 5'-DMT (dimethoxytrityl) for protecting the 5' end, a methylated deoxycytidine (mdC) residue, and a triethylene glycol (TEG) spacer linked to the ROX dye. The phosphoramidite is attached to controlled pore glass (CPG) with a 500 Å pore size. This configuration allows for the incorporation of a fluorescent marker into oligonucleotides, which is commonly used in fluorescence-based assays and detection methods. The TEG spacer provides flexibility and reduces steric hindrance, improving the dye's interaction with other molecules or probes in assays.
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Catalog
BRP-02330
Appearance
Purple powder
Storage
Store at -20 °C
Shipping
Room temperature.
Absorption Maximum (Lambda Max)
575
Fluorescence Maximum
602
Cleavage Conditions
Use t-butylamine/water (1:3) for 45 minutes at 25 °C. The use of ammonia must be avoided, as it will cause degradation of the ROX moiety.
Deprotection Conditions
When using fast deprotecting amidites (e.g., C-Ac; G-DMF; G-PAC), please use concentrated ammonia for 1h or AMA for 30 min at 60°C. When using standard amidites (e.g., C-Bz; G-iBu), please use concentrated ammonia for 5h at 60°C.
Chemical Structure:
