N Mix CPG (5'-DMT-[dA(Bz), dC(Ac), dG(iBu), T]-Suc-CPG); 1000 Å

N Mix CPG (5'-DMT-[dA(Bz), dC(Ac), dG(iBu), T]-Suc-CPG); 1000 Å is a reagent used in the solid-phase synthesis of oligonucleotides to incorporate a randomized mixture of the four nucleosides: deoxyadenosine (dA) with benzoyl (Bz) protection, deoxycytidine (dC) with acetyl (Ac) protection, deoxyguanosine (dG) with isobutyryl (iBu) protection, and thymidine (T). These nucleosides are linked via a succinyl (Suc) linker to controlled pore glass (CPG) with a 1000 Å pore size. The reagent is designed for synthesizing randomized oligonucleotide sequences, enabling the creation of diverse sequence libraries. The 5'-DMT group protects the 5' end during synthesis, ensuring controlled and efficient oligonucleotide assembly.

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Catalog

BRP-02323

Appearance

White to off-white powder

Storage

Store at 2-8 °C

Shipping

Room temperature.

Cleavage Conditions

Use concentrated ammonia for 90 min at 25°C or 30 min at 60°C, or 1:1 ammonia:methylamine (AMA) for 25 min at 25°C when using fast deprotecting amidites.

Deprotection Conditions

When using fast deprotecting amidites (e.g., C-Ac; G-DMF; G-PAC), please use concentrated ammonia for 1h or AMA for 30 min at 60°C. When using standard amidites (e.g., C-Bz; G-iBu), please use concentrated ammonia for 5h at 60°C.