Phosphoramidites

Description: 1-[(5E)-3-O-[(Bis-diisopropylamino)(2-cyanoethoxy)phosphino]-5,6-dideoxy-6-(diethoxyphosphinyl)-2-O-(2-MOE)-β-D-ribo-hex-5-enofuranosyl]uracil is a modified phosphoramidite compound used in oligonucleotides synthesis. This compound is primarily used in the solid-phase synthesis of RNA, where the protecting groups ensure that selective reactions occur at the desired sites. The phosphoramidite functionality facilitates the stepwise addition of nucleotides during automated oligonucleotide synthesis, allowing for the controlled assembly of RNA sequences.

CAT: BRP-02476

Molecular Formula: C26H44N4O10P2

Molecular Weight: 634.60

Description: 5'-O-DMTr-2'-O-Trifluoroacetamindo propyl-C(Ac)-3'-CE-Phosphoramidite is a highly modified cytidine phosphoramidite used in oligonucleotide synthesis. The 5'-O-DMTr (dimethoxytrityl) group serves as a protective group for the 5' hydroxyl during stepwise synthesis. The 2'-O-trifluoroacetamindo propyl modification adds a trifluoroacetamido group linked via a propyl spacer to the 2'-hydroxyl of the ribose, enhancing hydrophobicity and potentially altering enzymatic interactions or duplex stability. The C(Ac) designation indicates that the cytosine base is protected by an acetyl group at the N4 position, ensuring stability during synthesis. The 3'-CE phosphoramidite (cyanoethyl) group enables the coupling of this nucleotide into a growing oligonucleotide chain via phosphoramidite chemistry. This compound is often employed in the synthesis of modified oligonucleotides for research or therapeutic purposes, where specific modifications are required for stability, resistance to degradation, or unique biological functionality.

CAT: BRP-02480

Molecular Formula: C46H56F3N6O10P

Molecular Weight: 940.95

Description: 5'-O-DMTr-5-trifluoroacetylaminomethyl-2'-O-TBDMS uridine 3'-CED phosphoramidite is a chemically modified phosphoramidite designed for oligonucleotide synthesis. Its structure features a 5'-dimethoxytrityl (DMTr) group for temporary protection during solid-phase synthesis, a 5-position trifluoroacetylaminomethyl group to introduce functionalized side chains, a 2'-O-tert-butyldimethylsilyl (TBDMS) group for ribose protection, and a 3'-cyanoethyl diisopropyl phosphoramidite for coupling. This compound is used in the synthesis of RNA oligonucleotides, enabling the incorporation of modified residues for structural, therapeutic, or biochemical applications, such as enhancing stability or introducing specific reactive functionalities.

CAT: BRP-02487

Molecular Formula: C48H63F3N5O10PSi

Molecular Weight: 986.11

Purity: ≥95%

Description: 5'-O-DMTr-2'-O-(N-tri-fluoroacetylamino)ethyl-5-methyluridine 3'-CE-phosphoramidite is a modified phosphoramidite designed for oligonucleotide synthesis. The structure includes a 5-methyluridine base with a 2'-O-(N-tri-fluoroacetylamino)ethyl modification on the ribose, which enhances the molecule's stability and may influence its interaction with biological systems. The 5'-hydroxyl group is protected with a dimethoxytrityl (DMTr) group for efficient coupling during solid-phase synthesis, while the 3'-hydroxyl is protected with a cyanoethyl (CE) phosphoramidite group for coupling in the synthesis process. This modification improves the stability and specificity of the resulting oligonucleotides, making it suitable for use in RNA-based applications, including therapeutic and diagnostic purposes.

CAT: BRP-02490

CAS: 200423-98-9

Molecular Formula: C44H53F3N5O10P

Molecular Weight: 899.89

Purity: ≥95%

Description: It is a modified phosphoramidite designed for medicinal chemistry purposes, likely targeting specific enzymes or pathways in nucleotide metabolism, cell signaling, or as a potential therapeutic agent for diseases where phosphorothioate or phosphonate derivatives play a key role in inhibition or modulation.

CAT: BRP-02493

CAS: 2197181-94-3

Molecular Formula: C21H36N4O10P2

Molecular Weight: 566.48

Purity: ≥95%

Description: 1-O-(4,4'-Dimethoxytrityl)-3-O-succinyl-1,3-propanediol is a bifunctional molecule commonly used as a linker or spacer in the synthesis of oligonucleotides. The 1-hydroxyl group is protected with a 4,4'-dimethoxytrityl (DMT) group, which serves as a temporary protecting group during solid-phase synthesis, enabling stepwise addition of nucleotides. The 3-hydroxyl group is modified with a succinyl moiety, introducing a carboxylic acid functionality that allows covalent attachment to solid supports, such as controlled-pore glass (CPG) or polymers. This compound is critical in oligonucleotide synthesis, ensuring efficient attachment and controlled chain elongation during automated synthesis processes.

CAT: BRP-02496

CAS: 110916-49-9

Molecular Formula: C28H30O7

Molecular Weight: 478.53

Purity: ≥97%

Appearance: White or off-white solid

Description: It is a new diamine-scaffold-based phosphoramidite in the N-acetylgalactosamine (GalNAc)-siRNA conjugate that enhances siRNA delivery efficiency, particularly for liver targeting. It demonstrates superior silencing activity compared to the standard GalNAc L96 due to the incorporation of a PS-linkage at the 3' end, which increases resistance to nuclease degradation and prolongs gene silencing effects. This innovative structure provides a promising strategy for RNA-based therapeutics, especially in the treatment of undruggable diseases.

CAT: BRP-02497

CAS: 2766336-04-1

Molecular Formula: C90H150N13O42P

Molecular Weight: 2117.19

Description: It is a new diamine-scaffold structure that plays a significant role in enhancing the delivery and effectiveness of GalNAc-siRNA conjugated. It is often designed to improve the binding affinity of the siRNA to specific receptors, such as the asialoglycoprotein receptor (ASGPR) on hepatocytes (liver cells). By reinforcing the interaction between the conjugated siRNA and its target receptor, it ensures more efficient uptake of the siRNA into liver cells, which is pivotal for achieving therapeutic levels of gene silencing. Moreover, it may also include stabilizing modifications that protect the siRNA against degradation by nucleases, thus prolonging its half-life in circulation. Overall, it significantly contributes to the overall effectiveness of siRNA therapeutics by optimizing delivery to targeted tissues and enhancing their stability.

CAT: BRP-02498

Molecular Formula: C106H158N12O46

Molecular Weight: 2336.47

Description: It is a new diamine-scaffold structure that is designed to enhance the pharmacokinetic profiles of GalNAc-siRNA conjugated by providing increased metabolic stability. It often incorporates modifications that protect the siRNA from exonuclease degradation, which extends its half-life in circulation and improves its therapeutic efficacy. By ensuring more prolonged availability in the bloodstream, it contributes to the sustained release of siRNA at the target site, facilitating more effective gene silencing. Furthermore, it may also aid in optimizing the specificity and efficiency of liver-targeted delivery, making it valuable for the development of RNA-based therapeutics.

CAT: BRP-02499

Molecular Formula: C110H162N12O49

Molecular Weight: 2436.54

Description: It is a trivalent GalNAc precursor based on a diamine core, which serves as a crucial building block in the synthesis of GalNAc-siRNA conjugated. Its primary function is to facilitate the creation of ligands that can be efficiently attached to either the 5'-end or 3'-end of the sense strand of the oligonucleotide. By providing a versatile scaffold, it enables the development of siRNA molecules with enhanced liver-targeting capabilities due to the strong interactions of GalNAc with the asialoglycoprotein receptor (ASGPR). This targeted delivery is essential for improving the therapeutic efficacy of siRNA by promoting greater cellular uptake and allowing for effective silencing of the intended RNA targets.

CAT: BRP-02501

CAS: 2766335-88-8

Molecular Formula: C81H133N11O41

Molecular Weight: 1916.98

Description: 2'-TC-G(Ibu) Phosphoramidite is a modified phosphoramidite featuring a TC modification on the ribose sugar and a guanine base that is protected with an isobutyryl (Ibu) group at the exocyclic amine. The 2'-thio modification enhances the stability and resistance of the resulting oligonucleotides to nuclease degradation, improving their overall chemical stability. The isobutyryl protection on the guanine base ensures selective incorporation and prevents unwanted side reactions during the synthesis process. The 3'-cyanoethyl (CE) phosphoramidite group facilitates efficient addition of this modified nucleoside into oligonucleotides. This phosphoramidite is particularly useful for synthesizing oligonucleotides with enhanced stability, making it valuable for therapeutic applications, including antisense oligonucleotides and other RNA-based therapies.

CAT: BRP-02502

CAS: 1219089-77-6

Molecular Formula: C49H61N8O11PS2

Molecular Weight: 1033.17

Purity: ≥98%

Appearance: White to light yellow to light orange solid