5'-Biotin CE Phosphoramidite - CAS 135137-87-0

Catalog number: BRI-004

5'-Biotin Phosphoramidite for direct labeling of synthetic oligonucleotides has the following characteristics:

Soluble in acetonitrile at concentrations useful for DNA synthesis.
Include the DMT group used for cassette purification, which is essential for the preparation of biotinylated PCR primers because cross-contamination may occur during HPLC purification.
To develop diagnostic probes, biotin phosphoramidite can be branched to allow the introduction of multiple biotins at the 3'or 5'end.

* Please kindly note that our products are not to be used for therapeutic purposes and cannot be sold to patients.

Synonyms

DMT-Biotin-C6 Phosphoramidite

CAS

135137-87-0

IUPAC Name

5-[(3aR,6S,6aS)-3-[bis(4-methoxyphenyl)-phenylmethyl]-2-oxo-3a,4,6,6a-tetrahydro-1H-thieno[3,4-d]imidazol-6-yl]-N-[6-[2-cyanoethoxy-[di(propan-2-yl)amino]phosphanyl]oxyhexyl]pentanamide

Molecular Weight

846.08

Molecular Formula

C46H64N5O6PS

Canonical SMILES

CC(C)N(C(C)C)P(OCCCCCCNC(=O)CCCCC1C2C(CS1)N(C(=O)N2)C(C3=CC=CC=C3)(C4=CC=C(C=C4)OC)C5=CC=C(C=C5)OC)OCCC#N

InChI

InChI=1S/C46H64N5O6PS/c1-34(2)51(35(3)4)58(57-32-16-29-47)56-31-15-8-7-14-30-48-43(52)20-13-12-19-42-44-41(33-59-42)50(45(53)49-44)46(36-17-10-9-11-18-36,37-21-25-39(54-5)26-22-37)38-23-27-40(55-6)28-24-38/h9-11,17-18,21-28,34-35,41-42,44H,7-8,12-16,19-20,30-33H2,1-6H3,(H,48,52)(H,49,53)/t41-,42-,44-,58?/m0/s1

InChIKey

RBXYKQBFPLXSJS-RYQQVEDISA-N

Boiling Point

934.9±65.0 °C at 760 mmHg

Purity

>95% by HPLC

Appearance

Off-white Solid

Storage

Store at -20°C

Symbol

DMT-Biotin-C6 Amidite

Formulation

Dilute with anhydrous acetonitrile

Chemical Structure: