Spacer phosphoramidites introduce flexible arms into DNA or RNA sequences, altering conformation, reducing steric hindrance, and improving aptamer or label/tag accessibility.
Shorter spacers like C3 simulate small gaps between nucleotides, while longer spacers (C12, C18) reduce steric hindrance, increase hybridization efficiency, or protect ends from exonuclease activity.
Yes, they can be used with 3' or 5'-amino modifiers, fluorescent dyes, or multiple spacers sequentially to achieve precise spatial positioning of functional groups or reporter tags.
Photocleavable spacers allow controlled release of oligonucleotides or tags under light exposure, enabling temporal or spatial regulation in molecular assays.
Each batch undergoes HPLC and 31P NMR analysis to ensure high purity, minimal impurities, and reproducibility, with tightly monitored processes from material procurement to product release.
Yes, BOC RNA provides flexible custom manufacturing to develop novel spacers or tailor spacer length and chemistry to specific oligonucleotide synthesis requirements.
