Linker phosphoramidites introduce functional groups or reporter molecules, such as biotin or fluorescent dyes, at the 5'-end of oligonucleotides, enabling site-specific labeling or surface conjugation.
MMT linkers are acid-labile and useful for reversed-phase purification and coupling analysis, while TFA linkers are base-labile, allowing deprotection under basic conditions without additional steps.
They allow multiple oligonucleotides to be synthesized in a single solid-phase reaction, require lower reagent amounts, and reduce costs while maintaining controlled labeling density.
MMT deprotection is performed with acidic solutions or acetic acid at room temperature, and coupling efficiency can be monitored colorimetrically, ensuring high-purity modified oligonucleotides.
Yes, both MMT- and TFA-protected amino linkers are compatible with automated or manual oligonucleotide synthesis, allowing flexible incorporation of functional groups for research or diagnostic applications.
Each batch undergoes strict quality checks, including HPLC and 31P NMR analysis, with tightly controlled manufacturing processes to ensure minimal impurities and reproducibility between syntheses.
