BOC RNA provides the most complete Thiol-Modifier C6 S-S, which can be used to introduce 3'- or 5'-thiol bonds
Reference Reading
1.The p53-like Protein CEP-1 Is Required for Meiotic Fidelity in C. elegans.
Mateo AF1, Kessler Z2, Jolliffe AK1, McGovern O2, Yu B3, Nicolucci A3, Yanowitz JL4, Derry WB5. Curr Biol. 2016 Apr 13. pii: S0960-9822(16)30246-9. doi: 10.1016/j.cub.2016.03.036. [Epub ahead of print]
The passage of genetic information during meiosis requires exceptionally high fidelity to prevent birth defects and infertility. Accurate chromosome segregation during the first meiotic division relies on the formation of crossovers between homologous chromosomes and a series of precisely controlled steps to exchange genetic information. Many studies have hinted at a role for p53 in meiosis, but how it functions in this process is poorly understood. Here, we have identified a cooperative role for the p53-like protein CEP-1 and the meiotic protein HIM-5 in maintaining genome stability in the C. elegans germline. Loss of cep-1 and him-5 results in synthetic lethality that is dependent on the upstream DNA damage checkpoint but independent of the downstream core apoptotic pathway. We show that this synthetic lethality is the result of defective crossover formation due to reduced SPO-11-dependent double-strand breaks. Using cep-1 separation-of-function alleles, we show that cep-1 and him-5 also suppress inappropriate activation of the nonhomologous end joining (NHEJ) pathway.