BHQ-2 CPG; Glycolate, 500 Å
Catalog number: BRL-064
BHQ-2 CPG; Glycolate is used to add the non-fluorescent quencher BHQ-2 to the 3' end of oligonucleotides.
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Shipping
Room temperature.
Absorption Maximum (Lambda Max)
579
Cleavage Conditions
When using fast deprotecting amidites use concentrated Ammonia for 45 min at 25 °C or Ammonia/ Methylamine (AMA) (1:1) for 15 min at 25 °C; or t-butylamine/H2O (1:3) for 90 min at 25 °C (TAMRA requires deprotection with TBA). When using standard amidites (eg. C-Bz, G-iBu) use concentrated Ammonia for 45 min at 25 °C.
Deprotection Conditions
When using fast deprotecting amidites (e.g., C-Ac; G-DMF; G-PAC), please use concentrated ammonia for 1h or AMA for 30 min at 60°C. When using standard amidites (e.g., C-Bz; G-iBu), please use concentrated ammonia for 5h at 60°C.
Chemical Structure: