5'-DMT-dG(DMF)-Suc-CPG; 500 Å

Catalog number: BRL-034

5'-DMT-dG(DMF)-Suc-CPG is used to add unmodified dG to the 3' end of oligonucleotides.

* Please kindly note that our products are not to be used for therapeutic purposes and cannot be sold to patients.

Appearance

White powder

Storage

+2 to +8 °C.

Shipping

Room temperature.

Cleavage Conditions

Use concentrated ammonia for 90 min at 25°C or 30 min at 60°C, or 1:1 ammonia:methylamine (AMA) for 25 min at 25°C when using fast deprotecting amidites.

Deprotection Conditions

When using fast deprotecting amidites (e.g., C-Ac; G-DMF; G-PAC), please use concentrated ammonia for 1h or AMA for 30 min at 60°C. When using standard amidites (e.g., C-Bz; G-iBu), please use concentrated ammonia for 5h at 60°C.

Chemical Structure:

Reference Reading

1. Digital Microfluidic Cell Culture

Alphonsus H C Ng, Bingyu Betty Li, M Dean Chamberlain, Aaron R Wheeler. Review. 2015;17:91-112. doi: 10.1146/annurev-bioeng-071114-040808.

Digital microfluidics (DMF) is a droplet-based liquid-handling technology that has recently become popular for cell culture and analysis. In DMF, picoliter- to microliter-sized droplets are manipulated on a planar surface using electric fields, thus enabling software-reconfigurable operations on individual droplets, such as move, merge, split, and dispense from reservoirs. Using this technique, multistep cell-based processes can be carried out using simple and compact instrumentation, making DMF an attractive platform for eventual integration into routine biology workflows. In this review, we summarize the state-of-the-art in DMF cell culture, and describe design considerations, types of DMF cell culture, and cell-based applications of DMF.

2. Insight into Biomass Upgrade: A Review on Hydrogenation of 5-Hydroxymethylfurfural (HMF) to 2,5-Dimethylfuran (DMF)

Nor Azam Endot, Ramli Junid, Mohamad Shazwan Shah Jamil. Molecules. 2021 Nov 13;26(22):6848. doi: 10.3390/molecules26226848.

Recent developments in the transformation of biobased 5-hydroxymethylfurfural (HMF) into a potential liquid fuel, 2,5-dimethylfuran (DMF), are summarised. This review focuses briefly on the history of HMF conversion to DMF in terms of the feedstock used and emphasises the ideal requirements in terms of the catalytic properties needed in HMF transformation into DMF. The recent state of the art and works on HMF transformation into DMF are discussed in comparison to noble metals and non-noble metals as well as bimetallic catalysts. The effect of the support used and the reaction conditions are also discussed. The recommendations for future work and challenges faced are specified.

3. The deleterious effects of N,N-dimethylformamide on liver: A mini-review

Ming-Jun Li, Tao Zeng. Review. 2019 Jan 25;298:129-136. doi: 10.1016/j.cbi.2018.12.011.

N,N-dimethylformamide (DMF) is a versatile solvent with wide industrial applications. Evidences from animal studies and occupational poisoning cases have clearly demonstrated that DMF exposure can lead to different degrees of liver damage. It is noteworthy that DMF below the threshold limit value (TLV) may also cause liver injury in some sensitive populations. Unfortunately, the underlying mechanisms by which DMF induces hepatotoxicity remain largely unknown, despite considerable attention has been drawn to the hepatotoxic effects of DMF. Although some pilot studies have provided some evidences supporting the involvement of oxidative stress, the disturbance of gut microbiota and calcium homeostasis, etc, the causal roles of these factors on the onset of DMF-induced hepatotoxicity need to be confirmed. This article reviews the current knowledge about the deleterious effects of DMF on the liver.