Phosphoramidites are activated monomers used in solid-phase DNA and RNA oligonucleotide synthesis.
RNA phosphoramidites include additional 2′-hydroxyl protecting groups, making them more sensitive to moisture and handling conditions.
Moisture can cause premature hydrolysis of phosphoramidites, reducing coupling efficiency and synthesis yield.
Yes, high-quality phosphoramidites are essential for achieving good yields in long and complex oligonucleotide sequences.
Modified and specialty phosphoramidites are commonly used to introduce functional groups or labels into oligonucleotides.
