Custom MicroRNA Lentivirus Packaging Service

In the field of molecular biology, the precise modulation of gene expression is paramount for understanding biological processes and developing therapeutic interventions. One powerful tool in this endeavor is the use of lentiviral vectors, which allow for efficient delivery of genetic material into target cells. BOC Sciences proudly offers a Custom MicroRNA Lentivirus Packaging Service, tailored to meet the diverse needs of researchers in the exploration of gene function and therapeutic applications.

What is MicroRNA?

MicroRNAs (miRNAs) are small, non-coding RNA molecules approximately 22 nucleotides in length, found in animals, plants, and some viruses. They play crucial roles in post-transcriptional gene regulation by binding to target messenger RNA (mRNA) molecules, leading to degradation or translational repression. MiRNAs are involved in various biological processes, including development, differentiation, apoptosis, and disease progression.

What is Lentivirus Packaging?

Lentivirus is an enveloped RNA virus with a diameter of 80-120nm, exhibiting an icosahedral symmetric structure and a spherical shape. The outermost layer of the viral particle is the envelope (envelope protein determines the type of infected cells), followed by the matrix protein and the capsid, with the innermost layer consisting of two identical positive RNA strands and enzymes (reverse transcriptase, integrase, and protease). Lentivirus is a type of retrovirus with a double-stranded RNA genome. Unlike typical retroviruses, lentiviruses have a broader host range and can infect both dividing and non-dividing cells. Recombinant lentiviral vectors are developed based on HIV-1 (Human Immunodeficiency Virus Type 1) as a tool vector, with its virulence genes removed and replaced by exogenous target genes, making it a pseudovirus. The vector randomly integrates the target gene into the host genome and can stably express for several generations in cell lines, enabling the screening of stable cell lines. Widely used in various cell line gene knockout, gene overexpression, and RNA interference applications.

Schematic diagram of adeno-associated virus.

Packaging System

The lentiviral vector contains the genetic information required for packaging, transfection, and stable integration. The lentiviral packaging plasmid provides auxiliary proteins required for transcription and packaging of RNA into the recombinant pseudovirus vector.

• Envelope Glycoprotein: Recognizes and infects host cells.
• Structural Protein: Enzymes required for virus replication.

BOC Sciences' Custom MicroRNA Lentivirus Packaging Service

At BOC Sciences, we offer a comprehensive Custom MicroRNA Lentivirus Packaging Service to support researchers in their gene modulation studies. Our service includes the construction of microRNA overexpression/inhibition lentivector clones and the packaging of lentivirus for transduction experiments. BOC Sciences will prepare transduction-ready, high-quality, high-titer microRNA lentiviral formulations tailored to the production scale required for your project, even up to large scales of up to 10 mL.

Service Process of BOC Sciences' Custom MicroRNA Lentivirus Packaging Service

The process begins with the submission of the desired microRNA sequence or construct by the researcher. Our team of experts then amplifies and clones the microRNA precursor into a lentiviral vector, ensuring efficient overexpression or inhibition of the target miRNA. The lentiviral particles are produced, concentrated, and titrated to achieve high titers suitable for transduction experiments. Throughout the process, we maintain strict quality control measures to ensure the reliability and consistency of our lentivirus preparations.

• Quality Control Test Items

Features of BOC Sciences' Custom MicroRNA Lentivirus Packaging

• Stable Long-Term Expression of miRNA: Suitable for screening stable cell models for long-term research and observation.
• High Infection Efficiency: Capable of infecting a wide range of cell types with high efficiency.
• Direct Application in Animal Experiments: Can be directly used in animal experiments or administered to animals after infecting cells.

Applications of BOC Sciences' Custom MicroRNA Lentivirus Packaging Service

BOC Sciences' engineered miRNA lentiviral vectors enable the overexpression or inhibition of specific miRNA activity, facilitating the study of miRNA functionality in various biological processes and diseases, and providing insights into miRNA-mediated gene expression regulation. We are committed to providing global customers with miRNA lentivirus packaging services to advance basic research and the development of miRNA-based therapeutic strategies. Our Custom MicroRNA Lentivirus Packaging Service has broad applications in both basic research and therapeutic development. Researchers can utilize the lentivirus preparations for various gene modulation studies, including:

• Investigating the function of specific miRNAs in cellular processes
• Studying miRNA-mediated gene regulatory networks
• Modeling disease states and identifying potential therapeutic targets
• Screening for novel miRNA-based therapeutic interventions

Advantages of BOC Sciences' Custom MicroRNA Lentivirus Packaging Service

At BOC Sciences, we pride ourselves on delivering high-quality, reliable lentivirus preparations tailored to meet the unique needs of our customers. Our service offers several advantages:

• Expert Technical Support: Our team of experienced scientists provides expert guidance and support throughout the entire process, ensuring the success of your experiments.
• Customization: We offer customizable solutions to accommodate diverse research needs, including the construction of custom lentivectors and optimization of experimental conditions.
• Timely Delivery: We understand the importance of timely delivery in research projects. Our streamlined processes and efficient workflows enable us to deliver lentivirus preparations within a reasonable timeframe.
• Quality Assurance: We adhere to stringent quality control measures to ensure the purity, potency, and consistency of our lentivirus preparations, providing researchers with reliable tools for their experiments.
• Cost-Effectiveness: We offer competitive pricing and flexible service packages to accommodate different budgetary constraints, allowing researchers to access high-quality lentivirus preparations at affordable rates.

In conclusion, BOC Sciences' Custom MicroRNA Lentivirus Packaging Service offers researchers a comprehensive solution for their gene modulation studies, providing high-quality lentivirus preparations backed by expert technical support, customization options, and timely delivery. If you have custom synthesis needs for more types of miRNA Sponges, please feel free to contact us.

Reference

1. M, S, Ebert and P, A, Sharp. MicroRNA Sponges: Progress and Possibilities. RNA. 2010, 16(11): 2043–2050.