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mCherry saRNA (5mCTP) is a self-amplifying RNA designed to express the red fluorescent protein mCherry, derived from the sea anemone Discosoma. This saRNA features a Cap 1 structure and is modified with 5-methylcytidine triphosphate (5mCTP) to enhance transcription efficiency and suppress RNA-mediated innate immune activation. The addition of a poly(A) tail further increases mRNA stability and translation efficiency. mCherry has an excitation maximum at 587 nm and an emission maximum at 610 nm, making it highly photostable and suitable for live-cell imaging and protein localization studies.
Chemical Structure:
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